Volume 13, Issue 62 (vol. 13, no. 62 2024)
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Thymus vulgaris L. belongs to the Lamiaceae family, which is considered one of the most important medicinal and industrial plants. In the present study, the effects of foliar application of methyl jasmonate and titanium dioxide nanoparticles on the quantitative and qualitative characters of essential oil in Thyme based on a randomized complete block design with 3 replications in two growing seasons (2022 and 2023) were evaluated by two separate experiments in the Research Farm of the Islamic Azad University Branch of Shahrekord. Foliar application of methyl jasmonate (0, 0.5, 1, 2 mM) and titanium dioxide nanoparticles (0, 100, 200 and 300 mg. L-1) was performed three times with a 4-day interval after the V12-V20 stage. For measurement of the content and yield of the volatile oil, 1000g of the fresh shoots were harvested, mixed with water and placed in a flask connected to the condenser of a Clevenger apparatus and the GC analysis was done to detect the essential oil composition. In two years, the highest weight of dry matter (362.8-365.5 g.m-2), essential oil content (0.95-0.97%) and total chlorophyll (1.69-1.71 mg.dw-1) were observed in plants treated with 1 mM methyl jasmonate which was the same group as 100 mg L-1 titanium dioxide nanoparticles. Four components of Thymyl acetate, P-cymene, Gamma terpinen and Carvacrol are classified as cyclic monoterpene that make up more than 62% of the essential oil content in different treatments. Methyl jasmonate at a concentration of 1 mM increased the essential oil content of treated plants (0.97%) compared to control plants (0.69%) by 40%. According to the obtained results, applied methyl jasmonate at concentration of 1 mM is suggested to improve the quantitative and qualitative characters of essential oil in Thyme plants in same climate.
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