Volume 5, Issue 16 (Vol. 5, No. 16, year 2016 2016)                   2016, 5(16): 9-16 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

sobhannejad S, ghanati F. Effect of low-intensity ultrasound on membrane integrity of suspension-cultured parsley cells (Petroselinum crispum L.). Plant Process and Function 2016; 5 (16) :9-16
URL: http://jispp.iut.ac.ir/article-1-269-en.html
1- Tarbiat Modares University
2- Tarbiat Modares University , ghangia@modares.ac.ir
Abstract:   (5161 Views)

The ability to sense and respond to physical stimuli is an important key of the life. It was recognized that ultrasound as a physical stimulus could produce drastic changes in biological systems. High-intensity ultrasound is well known to be destructive to biological materials, but low-intensity ultrasound, on the other hand, has shown a range of biological effects with potential significance in biotechnology. One of the most studied effects of ultrasound on living cells is the increase in their membrane permeability, enhancing the uptake of foreign substances and the release of intracellular products into the media. In the present research suspension-cultured parsley cells were treated with ultrasound at 29 kHz with the power of 455 mW/cm3, for 10, 20, and 40 min. The viability of cells was examined using Evanꞌs blue dye. Alteration of membrane permeability was evaluated by measuring the lipid peroxidation rate and leakage of electrolytes such as Ca2+ and K+ from membranes to the extracellular medium. According to the results exposure to ultrasound for 10 and 20 min neither changed the viability of the cells nor lipid peroxidation of the membranes. Exposure of parsley cells to ultrasound for 40 min however, decreased the viability and increased the release of K+ to the extracellular medium. The results suggest that low dosage of ultrasound energy dose not adversely affect the membrane integrity of parsley cells and also stimulates their growth.

Full-Text [PDF 539 kb]   (2153 Downloads)    
Type of Study: Research | Subject: others
Received: 2014/07/4 | Accepted: 2015/08/25 | Published: 2016/07/10

References
1. Brodelius, P., Collinge, M. A., Funk, C., Gugler, K. and Marques, I. (1989) Studies on alkaloid formation in plant cell cultures after treatment with a yeast elicitor. In: Primary and Secondary Metabolism of Plant Cell Culture (ed. Kurz, W.G.W.) Pp. 190-199. Springer-Verlag, Berlin.
2. Chen B., Huang, J., Wang, J., Huang, L. (2008) Ultrasound effects on the antioxidative defense systems of Porphyridium cruentum. Colloids and surfaces B: Biointerfaces 61:88-92.
3. Ebel, J. and Mithorer, A. (1998) Early events in the elicitation of plant defense. Planta 206: 335-348.
4. Haar, G. (2007) Therapeutic applications of ultrasound. Progress in Biophysics and Molecular Biology 93: 111-129.
5. Hodges, D. M., DeLong, J. M., Forney, C. F. and Prange, R. K., (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering. Planta 207: 604-611.
6. Hoigne, D. J., Stubinger, S., Von Kaenel, O., Shamdasani, S., Hasenboehler, P. (2006). Piezoelectic osteotomy in hand surgery: first experiences with a new technique. BMC Musculoskeletal Disorders 7:36. doi:10.1186/1471-2474-7-36.
7. Labanca, M., Azzola, F., Vinci, R. and Rodella, L. F. (2008) "Piezoelectric surgery: twenty years of use". British Journal of Oral and Maxillofacial Surgery 46: 265-269.
8. Liu, Y., Takatsuki, H., Yoshikoshi, A., Wang, B., Sakanishi, A. (2003a) Effects of ultrasound on the growth and vacuolar H+-ATPase activity of aloe arborescens callus cells. Colloids and Surfaces B: Biointerfaces 32: 105-116.
9. Liu, Y., Yoshikoshi, A., Wang, B., Sakanishi, A. (2003b) Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nipponbare callus cells. Colloids and Surfaces B: Biointerfaces 27: 287-293.
10. Kumon, R. E., Aehle, M., Sabens, D., Parikh, P., Kourennyi, D. and Deng, C. X. (2007) Ultrasound-Induced Calcium oscillations and waves in chinese hamster ovary cells in the presence of microbubbles. Biophysical Journal: Biophysical Letters 93: L29-L31.
11. Loseva, N., Gordon, L., Alyabyev, A., Andreyeva, I., Kolesnikov, O., Chernov, V., Ponomareva, A. and Kemp, R. B. (2004) Effect of induced changes in membrane permeability on the defense response of Chlorella vulgaris to infection by Achole plasma laidlawii. Thermochimica Acta 422:95-100.
12. Mauch, F., Kmecl, A., Schaffrath, U., Volrath, S., Gorlach J., Ward, E., Ryals, J. and Dudler, R. (1997) Mechanosensitive expression of a lipoxygenase gene in wheat. Plant Physiology 114: 1561-1566.
13. Pickard, B. G., and Fujiki, M. (2005) Ca2+ pulsation in BY-2 cells and evidence for control of mechanosensory Ca2+-selective channels by the plasmalemmal reticulum. Functional Plant Biology 32: 863-879.
14. Pong, M., Umchid, S., Guarino, A. J., Lewin, P. A., Litniewski, J., Nowicki, A. and Wrenn, S. P. (2006) In vitro ultrasound-mediated leakage from phospholipid vesicles. Ultrasonics 45: 133-145.
15. Rajabbeigi, E., Ghanati, F., Abdolmaleki, P. and Payez, A. (2013) Antioxidant capacity of parsley cells (Petroselinum crispum L.) and static magnetic field in relation to iron-induced ferritin levels. Electromagnetic Biology and Medicine 32: 430-441.
16. Rezaei, A., Ghanati, F., Behmanesh, M. and Mokhtari, dizaji, M. (2011) Ultrasound-potentiated salicylic acid-induced physiological effects and production of taxol in hazel (Corylus avellana L.) cell culture. Ultrasound in Medicine and Biology 37:1938–1947.
17. Safari, M., Ghanati, F., Behmanesh, M., Hajnorouzi, A., Nahidian, B. and Ghahremani, M. (2013) Enhancement of antioxidant enzymes activity and expression of CAT and PAL genes in hazel (Corylus avellana L.) cells in response to low-intensity ultrasound. Acta Physiologia Plantarum 35:2847–2855.
18. Sanders, D., Pelloux, J., Brownlee, C. and Harper, J. F. (2002) Calcium at the crossroads of signaling. The Plant Cell 14: 401-417.
19. Smith, M., Palta, J. P., McCown, B. H. (1984) The measurement of isotonicity and maintenance of osmotic balance in plant protoplast manipulations. Plant Science Letters 33: 249-258.
20. Sundaram, J., Berlyn, R. M., and Mitragotri, S. (2003) An experimental and theoretical analysis of ultrasound-induced permeabilization of cell membranes. Biophysical Journal 84: 3087-3101.
21. Telewski, F. W. (2006) A unified hypothesis of mechanoperception in plants. American Journal of Botany 93: 1466-1476.
22. Wang, B. C., Yoshikoshi, A. and Sakanishi, A. (1998) Carrot cell growth response in a stimulated ultrasonic environment. Colloids and Surface B: Biointerfaces 12: 89-95.
23. Wu, J., Lin, L. (2002) Elicitor-like effects of low-energy ultrasound on plant (Panax ginseng) cells: induction of plant defense responses and secondary metabolite production. Applied Microbiology and Biotechnology 59: 51-57.

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Plant Process and Function

Designed & Developed by : Yektaweb