جلد 10، شماره 46 - ( جلد 10، شماره 46- بهمن و اسفند 1400 )
جلد 10 شماره 46 صفحات 40-29 |
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Torabi S, Karimi F, Razavi K. In vitro callus induction and multiple shoot regeneration in rare Iranian endemic plant Haplophyllum virgatum var. virgatum. Plant Process and Function 2022; 10 (46) : 4
URL: http://jispp.iut.ac.ir/article-1-1534-fa.html
URL: http://jispp.iut.ac.ir/article-1-1534-fa.html
Torabi Somayeh، Karimi Farah، Razavi Khadijeh. In vitro callus induction and multiple shoot regeneration in rare Iranian endemic plant Haplophyllum virgatum var. virgatum. فرآیند و کارکرد گیاهی. 1400; 10 (46) :29-40
1- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ، fkarimi@shahed.ac.ir
3- National institute of Genetic Engineering and Biotechnology, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ، fkarimi@shahed.ac.ir
3- National institute of Genetic Engineering and Biotechnology, Tehran, Iran
چکیده: (1011 مشاهده)
Haplophyllum virgatum var virgatum (Rutaceae) is a rare or narrow endemic plant, which grows only in one habitat (Geno-Hormozgan Province-Iran). Low distribution of this useful medicinal plant encouraged us to investigate the micropropagation conditions of the plant. In the present study, an efficient and reproducible protocol for multiple shoot induction of Haplophyllum virgatum var. virgatum was developed. Various explants (stem, leaf and root) were obtained from 40 day-old axenic seedlings and cultured on B5 medium supplemented with different concentrations of plant growth regulators (Kin, BA, NAA and IAA) to determine the suitable explants and media composition for callus production. BA and Kin were used for multiple shoot induction. Stem explants showed the best results in both callus induction and shoot regeneration. Combination of 0.1 mg L-1 Kin and 5 mg L-1 IAA produced the maximum callus fresh weight. BA at concentration of 2 mg L-1 was the best treatment for shoot induction and regeneration. Also, embryogenic callus was observed and the stages of globular and heart embryos were seen on callus culture in B5 medium supplemented with Kin (0.2 mg L-1) + IAA (1 mg L-1). In conclusion, stem explants and 2 mg L-1 BA regenerated maximum number of shoots.
شماره‌ی مقاله: 4
واژه‌های کلیدی: Callus culture، Haplophyllum virgatum، Organ culture، Root regeneration، Shoot regeneration
نوع مطالعه: گزارش مورد |
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دریافت: 1400/2/14 | پذیرش: 1400/6/10 | انتشار: 1400/11/12
دریافت: 1400/2/14 | پذیرش: 1400/6/10 | انتشار: 1400/11/12
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