Volume 10, Issue 46 (vol. 10, No. 46 2022)
2022, 10(46): 29-40 |
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Torabi S, Karimi F, Razavi K. In vitro callus induction and multiple shoot regeneration in rare Iranian endemic plant Haplophyllum virgatum var. virgatum. Plant Process and Function 2022; 10 (46) : 4
URL: http://jispp.iut.ac.ir/article-1-1534-en.html
URL: http://jispp.iut.ac.ir/article-1-1534-en.html
1- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ,fkarimi@shahed.ac.ir
3- National institute of Genetic Engineering and Biotechnology, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ,
3- National institute of Genetic Engineering and Biotechnology, Tehran, Iran
Abstract: (1365 Views)
Haplophyllum virgatum var virgatum (Rutaceae) is a rare or narrow endemic plant, which grows only in one habitat (Geno-Hormozgan Province-Iran). Low distribution of this useful medicinal plant encouraged us to investigate the micropropagation conditions of the plant. In the present study, an efficient and reproducible protocol for multiple shoot induction of Haplophyllum virgatum var. virgatum was developed. Various explants (stem, leaf and root) were obtained from 40 day-old axenic seedlings and cultured on B5 medium supplemented with different concentrations of plant growth regulators (Kin, BA, NAA and IAA) to determine the suitable explants and media composition for callus production. BA and Kin were used for multiple shoot induction. Stem explants showed the best results in both callus induction and shoot regeneration. Combination of 0.1 mg L-1 Kin and 5 mg L-1 IAA produced the maximum callus fresh weight. BA at concentration of 2 mg L-1 was the best treatment for shoot induction and regeneration. Also, embryogenic callus was observed and the stages of globular and heart embryos were seen on callus culture in B5 medium supplemented with Kin (0.2 mg L-1) + IAA (1 mg L-1). In conclusion, stem explants and 2 mg L-1 BA regenerated maximum number of shoots.
Article number: 4
Keywords: Callus culture, Haplophyllum virgatum, Organ culture, Root regeneration, Shoot regeneration
Type of Study: case report |
Subject:
others
Received: 2021/05/4 | Accepted: 2021/09/1 | Published: 2022/02/1
Received: 2021/05/4 | Accepted: 2021/09/1 | Published: 2022/02/1
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