Journal of Plant Process and Function

Heavy metals contamination of soil is a widespread global problem. One approach to reduce the accumulation of heavy metals in plants is using of different growing substrates. In order to investigate of different combinations substrate and heavy metals effect on physiological and biochemical traits in bean) cv. Sadri), an experiment was conducted. The first factor included four salt of heavy metals Cd(NO₃)₂, Pb(NO₃)₂, Ni(NO₃)₂ and CuSO₄) with 50 mg kg^-1 soil concentration and a control surface without metal and the second factor included of four levels of different growing substrates (control (farm soil), compost, vermicompost and poplar sawdust). The results showed that the heavy metals reduced the rate of photosynthesis and increased ion leakage, proline, soluble sugar and MDA compared to control. Use of vermicompost by developing the physical and chemical of soil statuse caused to increase chlorophyll content, instantaneous water use efficiency, stomatal conductance, photosynthesis rate and soluble sugar and also reduced the electrolyte leakage of pinto bean leaves. As a result, it could play a positive role to reduce the effects of heavy metal stress on plant physiological and biochemical traits of pinto bean.