In order to investigate the effects of simultaneous application of vermicompost and putrescine on reproductive characteristics and biochemical traits of greenhouse cucumbers greenhouse cv Yalda a factorial Pot experiment, based on completely randomized design with three replications was conducted in of Mohaghegh Ardabili University in 2014. Experimental treatments include Substrates containing different percentages of vermicompost (0, 20, 40 and 60 percentage) and different concentrations of putrescine (0, 0.5, 1 and 1.5 mM). Results showed that interaction of vermicompost and putrescine spraying were significant for all of traits except fruit number and days to flowering. As increases in vermicompost percentage medium in basal ,growth indices were significantly increased compared to control so that the highest yield was obtained by combination of 40% vermicompost and spraying 1 mM of putrescine. In bed containing 60% vermicompost the yield was reduced due to salinity effects of salt accumulation in organic matters. Sprayings with 1 mM putrescine via increases in proline content, protein and solube sugar partially compensated growth reduction resulted from salinity in beds containing high rate of vermicompost.