Volume 14, Issue 66 (vol. 14, no. 66 2025)
2025, 14(66): 287-302 |
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Babalou F, Jamei R, Jafari M. Optimization of hairy root induction and evaluation of phenolic compound production in turnip (Brassica rapa ssp. rapa). Plant Process and Function 2025; 14 (66) : 17
URL: http://jispp.iut.ac.ir/article-1-2158-en.html
URL: http://jispp.iut.ac.ir/article-1-2158-en.html
1- Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
2- Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran
2- Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran
Abstract: (31 Views)
The hairy root culture system has been endorsed as an excellent approach for the in vitro production of pharmaceutically important plant metabolites. This study was conducted in the Urmia University plant cell and tissue culture laboratory to optimize turnip hairy root cultures and assess the content of their phenolic compounds. To induce hairy roots, hypocotyl, cotyledon, and leaf explants from in vitro turnip seedlings were inoculated with three strains of Agrobacterium rhizogenes, including A7, A13, and ATCC 15834. Molecular confirmation of hairy roots was performed through polymerase chain reaction (PCR) analysis using rolA-B specific primers. Finally, the production of phenolic and antioxidant compounds in hairy root cultures was examined and compared with normal roots. Based on the results, the percentage of hair root induction was significantly affected by the type of bacterial strain and the type of explant. The maximum hairy root induction rate (37%) was obtained by inoculating leaf explants with A. rhizogenes strain A13. Significant differences were observed between hairy root lines regarding growth and biomass production. In hairy root lines, the biosynthesis rate of six types of phenolic acids (rosmarinic acid, chlorogenic acid, cinnamic acid, gallic acid, coumaric acid, and caffeic acid) and three types of flavonoids (quercetin, apigenin, and rutin) in HPLC analysis, and in general, the total phenol-flavonoid content and antioxidant activity, were significantly higher, compared to normal roots. According to the results, turnip hairy root culture can be considered an efficient system for the biosynthesis of secondary metabolites.
Article number: 17
Keywords: Agrobacterium rhizogenes, Antioxidant activity, Flavonoids, In vitro culture, Secondary metabolite production
Type of Study: Research |
Subject:
biotechnology
Received: 2024/12/22 | Accepted: 2025/02/4 | Published: 2025/06/22
Received: 2024/12/22 | Accepted: 2025/02/4 | Published: 2025/06/22
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