In this research the effect of four concentrations of nickel and copper (25, 50, 100, and 200 µM) on root and shoot length, fresh and dry weight, leaf surface, and photosynthetic pigments (Chlorophyll a, b and carotenoids), sugar, malondialdehyde, hydrogen peroxide content and antioxidant enzyme activity (CAT and APX) in Okra plants were evaluated. The results showed that in those plants which were treated with all concentrations of copper and nickel, the content of photosynthetic pigments and leaf surface were reduced significantly in comparison with control. In this investigation it was found that shoot and root length, dry weight and fresh weight of shoot and root which were treated with concentrations 50, 100, and 200 µM of cupper and 100 and 200 µM of nickel were reduced significantly when compared with the control. Content of soluble sugars in shoot and root plants which were treated with all concentrations of nickel and concentrations 50, 100, and 200 µM of cupper increased significantly in comparison with the control. Also, content of malondialdehyde, hydrogen peroxide content and antioxidant enzyme activity (CAT and APX) increased significantly in shoot and root plants which were treated with concentrations 50, 100, and 200 µM of copper and nickel when compared with control.

Keywords: Antioxidant enzymes, Malondialdehyde, Okra, Photosynthetic Pigments, Soluble Sugar

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