Volume 12, Issue 56 (vol. 12, no. 56 2023)
2023, 12(56): 285-298 |
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Ahmadi Pozveh A, Talebi M, Sayed Tabatabaei B E. The effect of L-glutamine and medium culture on hairy root culture of Dracocephalum kotschyi Boiss.. Plant Process and Function 2023; 12 (56) : 18
URL: http://jispp.iut.ac.ir/article-1-1856-en.html
URL: http://jispp.iut.ac.ir/article-1-1856-en.html
1- Department of Biotechnology, Collage of Agriculture, Isfahan University of Technology, Isfahan, Iran
2- Department of Biotechnology, Collage of Agriculture, Isfahan University of Technology, Isfahan, Iran ,mtalebi@iut.ac.ir
2- Department of Biotechnology, Collage of Agriculture, Isfahan University of Technology, Isfahan, Iran ,
Abstract: (825 Views)
Hairy root induction and production in Dracocephalum kotschyi Boiss. was investigated as an environmental and tissue culture technique for producing secondary metabolites. For this purpose, an in vitro experiment was carried out in the form of a completely randomized design (CRD) with four replicates. The best strain of Agrobacterium rhizogenes among MSU, A7 and A4-N strains from four-week-old leaves of D. kotschyi and the best age of leaves for inoculation with A. rhizogenes from two, three and four-week-old seedlings were chosen. In order to induce hairy roots, the most appropriate culture medium in the co-cultivation and post-co-cultivation phases was selected among the MS and MS culture media without macro elements (NH4NO3, KNO3, CaCl2, and KH2PO4) and the MS and MS culture media containing L-glutamine (0.1 g/L), respectively. The highest percent transgenic hairy root was observed in the MSU strain (90%), four-week old leaf explants of D. kotschyi seedlings (82.28%) and MS medium without macro elements in the co-cultivation stage, and MS medium containing L-glutamine in the post-co-cultivation stage (75.78%). PCR analysis also verified the transgenic nature of the hairy roots using rolC, aux1 and virD specific primers. The results showed that hairy root production in D. kotschyi depended on the type of A. rhizogenes strain, the age of the explant, and the MS medium. Nevertheless, the length of the produced hairy root in D. kotschyi did not depend on the type of A. rhizogenes strain and the age of the explant. The results of this study can be used in the production of medicinal compounds from this plant, such as rosmarie acid, through the optimized hairy root technique.
Article number: 18
Type of Study: Research |
Subject:
biotechnology
Received: 2023/01/27 | Accepted: 2023/04/11 | Published: 2023/11/7
Received: 2023/01/27 | Accepted: 2023/04/11 | Published: 2023/11/7
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