In order to investigate the effect of organic fertilizers including compost and manure on morphological, yield and qualitative characteristics of Rosa damascena Mill, an experiment was conducted in a complete randomized block design with 7 treatments in 3 replications in Sirjan city in 2018-2019. The treatments included compost (20, 25 and 30 tons per hectare) and manure (10, 15 and 20 tons per hectare) along with the control (without fertilizer application). Results showed that fertilizer in all of the investigated characteristics was significant. Mean comparison of data showed that 20 tons / hectare manure responded better regarding to canopy, height, leaf length and width, fresh and dry weight of flowers, number of flowers, phenol, chlorophyll, and antioxidant. On the other hand, 30 tons / ha compost  showed  maximum in number of branches, leaves per plant, fresh and dry weight per plant  and essential oil yield. Also, cluster analysis showed that 20 and 30 tons / hectare manure and compost were put in the same group. Based on the results, 20 and 30 tons / hectare manure and compost, respectively, had the maximum effect on morphological and qualitative characteristics of Rosa damascena Mill seedlings.