Journal of Plant Process and Function

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

---

 Stevia rebaudiana Bert (Asteraceae) is herbaceous plant with sweet leaves, which are the source of sweetener products. The present study focuses on effects of different combination of plant growth regulators on callus induction, callus cell growth and regeneration and volatile constituents from callus of stevia in tissue culture condition. The results of present study showed callus formation promoted in all of media contained phytohormones of different auxin/cytokinin based combinations. However, NAA/BA combinations are significantly more effective to callus initiation and the highest amount of callus was obtained in medium supplemented with 1.5:2 mg/L of NAA/BA. It was revealed that shoot induction in callus was happen at hormonal treatments as IBA/BA and IBA/KIN (0.5:1.5 mgl^-1). High rooting obtained in media supplemented with NAA/KIN (1.5:0.5 mgl^-1). The results of GC-MS analyses of volatile constituents of calli showed that the callus oil obtained in media supplemented with NAA/Kin (1.5:0.5 mgl^-1). The NAA/Kin treatment with 1:0.5 mgl^-1 concentration was dominated with squalene (40.50%) as main compound. The callus formed in media contained NAA/BA was enriched with Tricosane (38.57%). In addition, phytol (11.69%, 6.92%) was regarded as major constituent of the oil obtained from cultures supplemented with IBA/Kin and IBA/BA, respectively. It was concluded that there was a considerable differences in the essential oil profile of callus induced from different hormonal combinations. It is thought that various hormonal treatments may be tending to changes in metabolic pathways.
 

Keywords: Tricosane, Phytol, Stevia Rebaudiana, Tissue culture, Plant Growth Regulators.

Full-Text [PDF 653 kb]   (1598 Downloads)    

---