Volume 2, Issue 4 (Vol. 2, No. 4, year 2013 2013)
2013, 2(4): 65-73 |
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Modarres M, Lahooti. M, Asili J, Kafi. M, ramezani A. Optimizing leaf callus culture in Salvia leriifolia for phenolic acids production. Plant Process and Function 2013; 2 (4) :65-73
URL: http://jispp.iut.ac.ir/article-1-52-en.html
URL: http://jispp.iut.ac.ir/article-1-52-en.html
1- , M_modarres 70@yahoo.com
Abstract: (18119 Views)
Salvia leriifolia is a medicinal plant from Lamiaceae family native to Khorasan and Semnan Provinaces. This plant has medicinal properties such as antinociceptive, anti-inflammatory, antidiabetic, antimicrobial and Antioxidant which all have been confirmed by researchers. In this research, callus induction was established for production of phenolic acids. For this purpose, the leaf explants were cultured in MS medium supplemented with 2,4-D (0,1,2,3,4 mgL-1), KIN (0,0.3,1mgL-1), BAP (0,1,2,3,4,5,6 mgL-1) and NAA (0,1,2,3 mgL-1). Accumulation of phenolic acids was measured after 4 weeks of culture by HPLC. Results showed that the best treatments for callus induction and growth were at the BAP5 mgl-1+NAA5mgl-1 and 2, 4- D 3 mgl-1 + KIN1mgl-1. Accumulation of caffeic acid and salvianolic acid B in calli was higher than leaves of Salvia leriifolias. The highest rosmarinic acid concentration was found in the treatment BAP 5mgl-1+NAA5mgl-1 that were about 3 times higher than in leaves.
Type of Study: Research |
Subject:
biotechnology
Received: 2013/04/8 | Accepted: 2013/09/22 | Published: 2013/09/22
Received: 2013/04/8 | Accepted: 2013/09/22 | Published: 2013/09/22
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