Today Grafting is important because of its advantageous in increasing plant resistance to temperature stress and salinity in Cucurbitaceae. In order to investigated the effect of grafting of Cucumis sativus var. Super daminos on babol and Cucurbita moschata var Ferro and Cucumis sativus collected in Isfahan comparing with non-grafting and self grafting of Cucumis sativus var. Super daminos as a control an experiment was design. The experiment based on carried out as Pilot experiment based on complete randomized design (CRD) with 4 replications. Treatments were temperature included optimum temperature (27±2) and cold temperature (12±2) and cucumber (cucumis sativus var super daminos) in greenhouse of Isfahan University of Technology. Results was shown that the highest chlorophyll, mesophyll conductance, stomata resistance was in Babol. Fresh and dry weigh decreased in non-grafted and self grafted in low temperature but it did not change for Ferro and Babol. The lowest chlorophyll content was in non-grafted in both low and optimum temperature. Stomata resistance decreased in Ferro in low temperature compare with optimum and did not change in other rootstock between two temperatures significantly. Photosynthesis in Babol and transpiration in non-grafting, Ferro and Isfahan cucumber decreased but they are not change in other rootstock with decreasing temperature significantly. Generally, grafting on Ferro decreased the hazardous effect of low temperature on height, fresh and dry weight of shoot, photosynthesis and photosynthesis water efficiency and transpiration. Cucumber grafting on most of these rootstock can alleviated the deleterious effect of low temperature.
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