Journal of Plant Process and Function

Saint julien A is one of the important semi-dwarf rootstocks belonging to prunus family in terms of resistance to some common disease and pests in stone-fruits in addition to its high compatibility to different stone-fruit cultivars. In this study, the effects of methyl jasmonate, TIBA and scopoletin were studied on some parameters of shoot regeneration, IAA-Oxidase activity as well as improving some growth deficits such as eradication of sluggish grown of regenerated shootlets, under in vitro condition. Two types of explants were subjected to experiments: normal and sluggish grown explants. Results revealed that in the normal grown explants scopoletin and methyl jasmonate (1.0 mg/l) affected significantly the shoot number regeneration (4.12±0.17 and 3.18±0.18) and the shoot length (4.13±0.11 and 3.19±0.06 cm) respectively on MS medium. These chemicals have had also significant effects on mean shoot number (3.5±0.2 and 2.06±0.06) and shoot length (1.77±0.02 and 1.45±0.03cm) respectively in the sluggish grown explants compared to control and TIBA. Results also indicated a considerable increase in IAA-Oxidase activity for sluggish grown shoots in comparison with control. Besides, the incorporation of TIBA to media decreased significantly the activity in IAA-Oxidase in both normal and slow grown explants along with 30 days.