Effects of iron and copper nanoparticles on stress factors and rosmarinic acid synthesis in moldavian balm (Dracocephalum moldavica L.)

Varasteh Khanlari, Nafise; Kiarostami, Khadijeh; Hosseinzadeh Namin, Monir; Abdoli, Mohammad

doi:DOI: 10.22034/13.60.429

Volume 13, Issue 60 (vol. 13, no. 60 2024) 2024, 13(60): 429-448 | Back to browse issues page

‎ DOI: 10.22034/13.60.429

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Varasteh Khanlari N, Kiarostami K, Hosseinzadeh Namin M, Abdoli M. Effects of iron and copper nanoparticles on stress factors and rosmarinic acid synthesis in moldavian balm (Dracocephalum moldavica L.). Plant Process and Function 2024; 13 (60) : 25
URL: http://jispp.iut.ac.ir/article-1-1985-en.html

Effects of iron and copper nanoparticles on stress factors and rosmarinic acid synthesis in moldavian balm (Dracocephalum moldavica L.)

Nafise Varasteh Khanlari¹

, Khadijeh Kiarostami ^*

², Monir Hosseinzadeh Namin¹

, Mohammad Abdoli³

1- Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2- Department of Plant Sciences, Faculty of Biological Sciences, Alzahra University, Tehran, Iran , kh.kiarostami@alzahra.ac.ir
3- Department of Plant Production and Genetics, Faculty of Agriculture, Malayer University, Malayer, Iran

Abstract: (287 Views)

Moldavian balm (Dracocephalum moldavica L.) is an annual herbaceous and aromatic plant from the Lamiaceae family. The present study aimed to investigate the individual and combined effects of different concentrations of iron nanoparticles (0, 250, 500 and 1000 ppm) and copper (0, 25, 50 and 100 ppm) on 3-month-old D. moldavica plants and choosing the best treatment to increase the amount of rosmarinic acid and antioxidant activity. The experiment was conducted based on a completely randomized design with 16 treatments and three replications in greenhouse conditions using the leaf spray method. TEM images were used for confirmation of nanoparticle penetration into the leaf tissue of D. moldavica plant. Rosmarinic acid content was measured using an HPLC device. The results showed that the effect of the treatments on the amount of photosynthetic pigments, anthocyanins, phenolic compounds, flavonoids, malondialdehyde (MDA), hydrogen peroxide (H₂O₂), proline, and antioxidants in D. moldavica was highly significant (P˂0.01) and the best results were obtained by the combined effect of the Fe (1000 ppm) + Cu (100 ppm) treatment. In the best treatment, the amounts of chlorophyll a, chlorophyll b and carotenoid were 90.9, 125 and 91.6%, respectively; anthocyanin was 73.9%; phenolic compounds and flavonoids were 74.1 and 57.1%; MDA, H₂O₂ and proline 114, 80.9 and 43.5% and rosmarinic acid increased by 465% compared to the control. Also, in the mentioned combined treatment, the amount of antioxidant activity decreased by 56.9% compared to the control. There was a positive and significant correlation (P˂0.01) between the mount of rosmarinic acid production and other traits, except antioxidant activity (r = -0.958**).

Article number: 25

Keywords: Dracocephalum moldavica L., Reactive oxygen species, Elicitor, Secondary metabolies, Transmission electron microscope

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Type of Study: Research | Subject: Toxic ions stress
Received: 2023/11/16 | Accepted: 2024/01/15 | Published: 2024/06/18

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