Volume 13, Issue 59 (vol. 13, no. 59 2024)
2024, 13(59): 179-192 |
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Rezapour A, Dehestani-ardakani M, Kamali K, Nasiri J, Meftahizadeh H. Optimizing in vitro micropropagation of Begonia rex using plant growth regulators. Plant Process and Function 2024; 13 (59) : 11
URL: http://jispp.iut.ac.ir/article-1-1917-en.html
URL: http://jispp.iut.ac.ir/article-1-1917-en.html
1- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran
2- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran; Medicinal and Industrial Plant Research Institute, Ardakan, I.R. Iran ,mdehestani@ardakan.ac.ir
3- Department of Soil Science, Faculty of Agriculture & Natural Resources, Yazd University, Yazd, Iran
4- Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj, Iran
5- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran; Medicinal and Industrial Plant Research Institute, Ardakan, I.R. Iran
2- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran; Medicinal and Industrial Plant Research Institute, Ardakan, I.R. Iran ,
3- Department of Soil Science, Faculty of Agriculture & Natural Resources, Yazd University, Yazd, Iran
4- Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj, Iran
5- Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran; Medicinal and Industrial Plant Research Institute, Ardakan, I.R. Iran
Abstract: (604 Views)
This research was conducted to optimize in vitro micropropagation of Begonia rex as an important ornamental pot plant. In vitro callogenesis and regeneration of three cultivars of B. rex (Jurassic, Silver dollar and Silver king) were carried out starting from leaf segments as explants. Eight Murashige and Skoog′s media consisted of four plant growth regulators, including α-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), 3-Indole-3-butyric acid (IBA), and Thidiazuron (TDZ). After four weeks, callogenesis and regeneration started. After eight weeks of proliferation, the explants were transferred to MS culture medium supplemented with 0.05 mg/l gibberellic acid. The highest direct regeneration percent occurred in the medium containing 0.5 mg/L BA + 0.2 mg/L TDZ. Callogenesis, regeneration and root formation occurred in all treatments. The highest number of leaves was observed in the culture medium supplemented with 1 mg/L IBA + 1 mg/L BA in the Jurassic cultivar. Medium fortified with 0.2 mg/L NAA + 0.2 mg/L BA and 1 mg/L IBA + 1 mg/L BA was selected as the best medium for increasing the fresh weight and proliferation of cv. Jurassic. The highest amounts of chlorophyll a and b were obtained in 0.5 mg/l BA + 0.2 mg/l TDZ and 1 mg/l of IBA + 1 mg/l of BA treatments in ‘Jurassic’ and ‘Silver dollar’ cultivars. The survival rate of transplanted plant lets was about 100%. Generally, the combination of 1 mg/l IBA with 1 mg/l BA could be introduced as an effective and useful treatment in the micropropagation of studied three cultivars of Begonia rex.
Article number: 11
Type of Study: Research |
Subject:
Horticulture
Received: 2023/05/29 | Accepted: 2023/10/9 | Published: 2024/04/29
Received: 2023/05/29 | Accepted: 2023/10/9 | Published: 2024/04/29
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