Volume 10, Issue 45 (vol. 10, no. 45 2021)
2021, 10(45): 165-174 |
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Khorshidi M. An investigation of some concentrations of growth regulators (BAP, GA3, NAA and IBA) in micropropagation of myrobalan 29c (Prunus cerasifera Ehrh.). Plant Process and Function 2021; 10 (45) : 11
URL: http://jispp.iut.ac.ir/article-1-1495-en.html
URL: http://jispp.iut.ac.ir/article-1-1495-en.html
Department of Biology, Damghan University, Damghan, Iran. , m_khorshidi@du.ac.ir
Abstract: (1456 Views)
Stone fruits are valuable because of their high nutritions and therefore propagation of the same rootstocks is vital in horticulture. The aim of the present study was to investigate some concentrations of growth regulators such as benzylaminopurine (BAP), gibberellic acid (GA3), naphthalene acetic acid (NAA), indole butyric acid (IBA) in shoot germination and rooting of myrobalan 29c. This experiment was performed in a completely randomized block design with three replications (at least 5 samples per replication) in 14 experimental groups (G1-G14). In this study, MS culture medium and growth regulators of BAP (0, 0.5, 1 and 2 mg/L), GA3 (0, 0.1 and 0.2 mg/L), NAA (0 and 0.1 mg/L) were used in shoot generation. The results showed that shoot generation varied between 1.05 in G1 group to 8.27 in G6 group. The shoot length varied between 2.87 cm in G5 group to 4 cm in G6 group. The best shoot generation was observed at concentrations of 1 mg/L BAP, 0.1 mg/L NAA and 0.2 GA3 at 8.27 numbers. The 1/2 MS medium and growth regulators of IBA (0, 0.25, 0.5 and 1 mg/L) and NAA (0, 0.25, 0.5 and 1 mg/L) were used for rooting. The results showed that rooting varied between zero numbers in the G1 group to 7.6 numbers with 8.78 cm length in the G13 group. The best rooting was obtained at 1/2MS with 0.5 IBA and 0.25 NAA concentration with 7.6 root numbers.
Article number: 11
Keywords: Benzyl aminopurine, Gibberellic acid, Indole butyric acid, Micropropagation, Myrobalan, Naphthalene acetic acid.
Type of Study: Research |
Subject:
Plant Growth Regulators
Received: 2021/01/4 | Accepted: 2021/05/11 | Published: 2021/12/20
Received: 2021/01/4 | Accepted: 2021/05/11 | Published: 2021/12/20
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