Volume 9, Issue 38 (vol. 9, no. 38 2020)
2020, 9(38): 49-59 |
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Mousavimatin S, Mortazavi S N, Azadi P, Aelaei M. Optimization of callus production and organogenesis of two commercial cultivars of Gladiolus (Gladiolus grandiflorus L. cv Amsterdam, Advance Red). Plant Process and Function 2020; 9 (38) :49-59
URL: http://jispp.iut.ac.ir/article-1-1284-en.html
URL: http://jispp.iut.ac.ir/article-1-1284-en.html
1- University of Zanjan
2- Agricultural Biotechnology Research Institute of Iran ,azadip22@gmail.com
3- Department of Horticulture Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2- Agricultural Biotechnology Research Institute of Iran ,
3- Department of Horticulture Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Abstract: (1849 Views)
Gladiolus is cultivated as a high-value ornamental plant in the worldwide and it has the highest cultivation area and the highest level of underground organs imports in Iran. In this study, in order to investigate indirect regeneration of Amsterdam and Advance Red cultivars an experiment was conducted in a completely randomized design with three replications. For callogenesis, bud sprout (apical section), corm slices (middle section) and basal plate (bottom section) explants of corm with 1 cm2 dimensions were cultured in MS medium with different concentrations of Picloram, Dicamba, BAP , kinetin, NAA and 2,4,5-T. At the regeneration stage, different concentrations of TDZ and GA3 were combined with Proline and Glutamine amino acids. Based on the results, in the medium containing 1 or 2 mg L-1 of Dicamba, the bud sprout explant showed the highest callogenesis (77.77% and 75.91% in Amsterdam and Advance Red, respectively). The highest percentage of regeneration (81.11% and 75.57% in Amsterdam and Advance Red, respectively) and the highest number of shoots (23.69 and 20.19 in Amsterdam and Advance Red, respectively) were obtained in 1 mg L-1 TDZ + 0.5 mg L-1 GA3 + 200 mg L-1 Proline + 200 mg L-1 Glutamine. The highest percentage of rooting (84.41% and 82.50% in Amsterdam and Advance Red, respectively) were produced in MS medium supplemented with 2 mg L-1 IAA + 500 mg L-1 activated charcoal. Produced plantlets from indirect regeneration were successfully adapted in greenhouse condition.
Type of Study: Research |
Subject:
biotechnology
Received: 2019/07/23 | Accepted: 2020/01/22 | Published: 2020/11/7
Received: 2019/07/23 | Accepted: 2020/01/22 | Published: 2020/11/7
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