Journal of Plant Process and Function

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Emamian Tabarestani M, Pirdashti H, Tajik Ghanbary M A, Sadeghzadeh F. Quantifying the response of growth, some physiological and biochemical traits of canola (Brassica napus L.) plant to lead concentration in the soil. Plant Process and Function 2020; 9 (36) :453-468
URL: http://jispp.iut.ac.ir/article-1-1126-en.html

In order to quantify the response of canola (Brassica napus L.) plant to lead different levels, an experiment was conducted in completely randomized design with three replications. Treatments were twelve lead concentrations from lead nitrate source (0, 10, 50, 100, 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000 mg/kg soil). Results showed that decrease in vegetative traits fitted with segmented model when lead concentration increased. Also, chlorophyll a, b, a+b contents and SPAD index value declined about 23, 47, 30 and 22%, respectively. Chlorophyll a/b ratio (a/b) and carotenoid, however, showed increasing trend by 46 and 39% in 4000 mg/kg of soil as compared to the control. In addition, the activity of catalase, peroxidase and ascorbate enzymes increased until about 10, 447 and 18 mg/kg of soil, respectively and then decreased when lead concentration was increased. Superoxide dismutase activity in 4000 mg/kg of soil was about 2.3 times greater than the control. Also, malon-di-aldehyde (MDA) and H₂O₂ concentrations in 4000 mg/kg of soil were about two times and proline concentration was about 12% greater than the control. In conclusion, up to 100 mg/kg of soil vegetative traits and photosynthetic pigments particularly showed the highest sensitivity. Among photosynthetic pigments, the sensitivity of chlorophyll b was higher than chlorophyll a. Also, the higher activity of superoxide dismutase enzyme than the other enzymes and proline can be a possible reason for ameliorating the damage of high levels of lead in canola.