Volume 8, Issue 33 (vol. 8, no. 33, 2020 2019)
2019, 8(33): 339-358 |
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Saljougi S, ranjbar M. An investigating of the interaction of zinc and copper on the accumulation of elements, antioxidant enzymes, photosynthetic pigments and malon dialdehyde in basil (Ocimum basilicum). Plant Process and Function 2019; 8 (33) :339-358
URL: http://jispp.iut.ac.ir/article-1-1002-en.html
URL: http://jispp.iut.ac.ir/article-1-1002-en.html
1- Department of Biology, Falavarjan branch, Islamic Azad University, Isfahan, Iran
2- Department of Biology, Falavarjan branch, Islamic Azad University, Isfahan, Iran ,ranjbar@iaufala.ac.ir
2- Department of Biology, Falavarjan branch, Islamic Azad University, Isfahan, Iran ,
Abstract: (2458 Views)
Zinc and copper are essential elements for plant growth and development. These two elements act as heavy metal in high concentrations and cause oxidative stress. The aim of this study was to investigate the effect of these elements on (Ocimum basilicum). This plant is an important herb of medicine and nutrition. In this study, seeds were planted in pots containing cocopeat and perlite and the four-leaf seedlings were treated with zinc sulfate at of 0, 350 and 550 micro-molar concentrations and 0, 20 and 40 micro-molar copper sulfate in three replications. After a month, accumulation of the elements including, proline, soluble sugars, total proteins, photosynthetic pigment and malon dialdehyde contents, leaf area, number of leaves were investigated. The results showed that different concentrations of copper and zinc effects were not significant on germination percentage, number of leaves and stem length. However with increasing zinc and copper, the fresh and dry weight of the root, stems were up to 0.540, 0.120, 0.182 and 0.160 grams and the leaf area of the plant was up to 172.6 square centimeters in 40 micromolar of copper sulfate without zinc. Also, the content of malon dialdehyde (0.187 μMol/g) and Proline at a concentration of 550 micromolar of zinc with both concentrations of copper, the amount of soluble sugar, total protein at a concentration of 550 micromolar of zinc and 40 micromolar of copper increased. Antioxidant enzymes (catalase and peroxidase) did not activate these concentrations. The amount of photosynthetic pigments decreased at high concentrations of zinc and copper so that the total chlorophyll content turned to 79.9 mg/g. With increased concentrations of copper and zinc in the medium, copper and zinc accumulation increased in the root and shoot. Of course, the accumulation in the roots was more than the shoot. Increasing the high levels of zinc and copper in the medium reduced the amount of copper accumulation in the stem, root and zinc in the stem which confirms the existence of interaction between two elements. Increasing each of these two elements within the range of mentioned concentrations did not have an adverse effect on this plant. But their interactions had physiological and biochemical changes in the plant. Accordingly, the optimal concentration of these two elements in a basil was zinc at 350 μm with two concentrations of copper.
Type of Study: Research |
Subject:
Toxic ions stress
Received: 2018/05/29 | Accepted: 2018/11/24 | Published: 2020/05/11
Received: 2018/05/29 | Accepted: 2018/11/24 | Published: 2020/05/11
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