Volume 14, Issue 68 (vol. 14, no. 68 2025)
2025, 14(68): 19-32 |
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Ghandali M, Danaee E. The effect of ascorbic acid and thiamine on biochemical and enzymatic traits of basil (Ocimum basilicum L.). Plant Process and Function 2025; 14 (68) : 2
URL: http://jispp.iut.ac.ir/article-1-2138-en.html
URL: http://jispp.iut.ac.ir/article-1-2138-en.html
1- Department of Horticulture Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran
2- Department of Horticulture Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran ,Avin.ghandali@gmail.com
2- Department of Horticulture Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran ,
Abstract: (130 Views)
In order to investigate the effect of foliar spraying of ascorbic acid and thiamine on the morphophysiological, biochemical, and enzymatic traits of basil (Ocimum basilicum L.), an experiment in the form of a completely randomized statistical design with 3 replications in a commercial greenhouse located in Garmsar city was conducted in 2023. The treatments included foliar spraying of ascorbic acid and thiamine, each with three levels of 50, 100, and 150 mg/L. Foliar spraying of plants that were in the 6 to 8 leaf stage twice a week was done for two weeks. At the end of the experiment and after 40 days from the first foliar spraying, sampling and evaluation of traits were done. The results showed that the treatments had a significant effect on the evaluated traits. Thus, the highest percentage of cell membrane stability index, total chlorophyll content, amount of vitamin C, catalase, superoxide dismutase, and peroxidase enzyme activity were in the ascorbic acid 150 mg/liter treatment. The highest plant height was obtained in the thiamine 100 mg/liter treatment. Also, the use of thiamine 150 mg/liter increased the fresh and dry weight of shoots and roots and the amount of protein and thiamine. The highest and lowest percentages of green basil essential oil were 0.53% and 0.21% in thiamine 150 mg/L and control treatments, respectively. Therefore, the use of ascorbic acid and thiamine with concentrations of 100 and 150 mg/liter is recommended due to considering their important role in inhibiting oxygen free radicals and improving the morphological, biochemical, and enzymatic traits of green basil (Ocimum basilicum L.).
Article number: 2
Type of Study: Research |
Subject:
Horticulture
Received: 2024/11/12 | Accepted: 2025/02/11 | Published: 2025/10/29
Received: 2024/11/12 | Accepted: 2025/02/11 | Published: 2025/10/29
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