Volume 14, Issue 68 (vol. 14, no. 68 2025)
2025, 14(68): 133-154 |
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Zandi Toghan F, Sobhaniyan H, Yazdanpanah E, Bakshi Khaniki G, Ebrahimi M A. The effect of seed priming with zinc and iron elements on the germination parameters, morphological, physiological, and biochemical traits, and CBDAS and THCAS expression in Cannabis sativa L. plants under salt stress. Plant Process and Function 2025; 14 (68) : 9
URL: http://jispp.iut.ac.ir/article-1-2096-en.html
URL: http://jispp.iut.ac.ir/article-1-2096-en.html
Fateme Zandi Toghan1 
, Hamid Sobhaniyan *2 , Enayatollah Yazdanpanah1 , Gholamreza Bakshi Khaniki1 , Mohammad Ali Ebrahimi1
, Hamid Sobhaniyan *2 , Enayatollah Yazdanpanah1 , Gholamreza Bakshi Khaniki1 , Mohammad Ali Ebrahimi1
1- Department of Biology, Payame Noor University (PNU), P.O. Box 19395-4697, Tehran, Iran
2- Department of Biology, Payame Noor University (PNU), P.O. Box 19395-4697, Tehran, Iran ,Motif3000@yahoo.com
2- Department of Biology, Payame Noor University (PNU), P.O. Box 19395-4697, Tehran, Iran ,
Abstract: (119 Views)
The use of seed priming with micronutrients can be a promising approach in reducing the effects of salinity stress in cannabis plants. For priming, seeds were exposed to ZnSO4.7H2O solution with concentrations of 10, 50, and 100 mM and to FeSO4.7H2O with concentrations of 25, 50, and 75 mM. Salinity treatments were applied by adding 2 mL of 50 mM (mild) and 100 mM (severe) sodium chloride for 3 days in the Petri dish. The seedlings, then, were transferred from Petri dishes to plastic pots, and there they were watered with 50 (mild) and 100 (severe) mM NaCl solution. Data were analyzed based on a factorial experiment with a completely randomized design in three repeats. Salinity stress decreased seedling length, seedling dry weight, germination percentage, seed vigor, plant dry weight, total protein, chlorophyll, carotenoid, and antioxidant enzyme activity, while it increased electrolyte leakage and proline content. On the other hand, priming of cannabis seeds with iron and zinc micronutrients led to an increase in membrane stability and compatible osmolytes such as proline, protection of photosynthetic pigments, and improved activity of superoxide dismutase, catalase, and ascorbate peroxidase. The expression of CBDAS and THCAS genes was affected by salinity; different concentrations of iron and zinc were able to increase the expression of these genes and enable the production of more of their products in the cell. The combined treatment of 50 mM zinc and 75 mM iron was able to produce the best results in most cases. Considering the positive effect of iron and zinc on the cannabis plant, it is recommended to use a combined concentration of 50 mM zinc and 75 mM iron to improve the growth of cannabis under salt stress.
Article number: 9
Type of Study: Research |
Subject:
Plant Growth Regulators
Received: 2024/07/25 | Accepted: 2025/03/11 | Published: 2025/10/29
Received: 2024/07/25 | Accepted: 2025/03/11 | Published: 2025/10/29
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