Plant Process and Function

en Impact of Arthrospira platensis biostimulant on the cell biomass and antioxidant defense system of Matricaria chamomilla cell culture Impact of Arthrospira platensis biostimulant on the cell biomass and antioxidant defense system of Matricaria chamomilla cell culture بیوتکنولوژی biotechnology پژوهشي Research <div style="text-align: left;">Algal biostimulants are a promising strategy to improve crop yield and plant bioactive compounds. Arthrospira platensis (Spirulina) alga has received attention due to its low cost of cultivation and nontoxicity to induce plant growth and development. Optimizing cell culture mediums using biostimulants can be applied to scale up cell biomass in medicinal plants. This study examined the impact of various concentrations of Spirulina extract (0, 20, 40, and 80 mg L-1) on Matricaria chamomilla cell growth and antioxidant defense system after three weeks. The algal extract markedly increased the fresh and dry weights of M. chamomilla, and the maximum contents were obtained at 40 mg L-1 by a 2.7 and 2.3-fold raise compared to the control, respectively. Protein and carbohydrate contents increased by raising the extract concentrations, and the highest contents were observed in cells treated with 80 mg L-1 extract. H2O2 content decreased significantly in cells treated with 40 and 80 mg L-1 extract due to enhanced antioxidant enzymes of superoxide dismutase, catalase, and peroxidase activities. Moreover, the algal extract (80 mg L-1) maximized total phenolics (1282.7 mg GAE g-1 DW), flavonoids (790.6 mg g-1 DW), and DPPH scavenging activity (89.5%) in M. chamomilla cells. These findings demonstrate that A. platensis extract at 80 mg L-1 is a suitable concentration to scale up the cell biomass and secondary metabolite production in M. chamomilla cells.   </div> Algal biostimulants are a promising strategy to improve crop yield and plant bioactive compounds. Arthrospira platensis (Spirulina) alga has received attention due to its low cost of cultivation and nontoxicity to induce plant growth and development. Optimizing cell culture mediums using biostimulants can be applied to scale up cell biomass in medicinal plants. This study examined the impact of various concentrations of Spirulina extract (0, 20, 40, and 80 mg L-1) on Matricaria chamomilla cell growth and antioxidant defense system after three weeks. The algal extract markedly increased the fresh and dry weights of M. chamomilla, and the maximum contents were obtained at 40 mg L-1 by a 2.7 and 2.3-fold raise compared to the control, respectively. Protein and carbohydrate contents increased by raising the extract concentrations, and the highest contents were observed in cells treated with 80 mg L-1 extract. H2O2 content decreased significantly in cells treated with 40 and 80 mg L-1 extract due to enhanced antioxidant enzymes of superoxide dismutase, catalase, and peroxidase activities. Moreover, the algal extract (80 mg L-1) maximized total phenolics (1282.7 mg GAE g-1 DW), flavonoids (790.6 mg g-1 DW), and DPPH scavenging activity (89.5%) in M. chamomilla cells. These findings demonstrate that A. platensis extract at 80 mg L-1 is a suitable concentration to scale up the cell biomass and secondary metabolite production in M. chamomilla cells.   Antioxidant enzymes, Algal extract, Cell culture, Chamomile, Flavonoid Antioxidant enzymes, Algal extract, Cell culture, Chamomile, Flavonoid 33 42 http://jispp.iut.ac.ir/browse.php?a_code=A-10-241-7&slc_lang=en&sid=1 Halimeh Hassanpour Halimeh Hassanpour hassanpour@ari.ac.ir Yes Aerospace Research Institute, Ministry of Science Research and Technology, Tehran 14665 834, Iran Aerospace Research Institute, Ministry of Science Research and Technology, Tehran 14665 834, Iran Niusha Barati Niusha Barati Niusha.barati@yahoo.com No Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Sara Pourhabibian Sara Pourhabibian Sara Pourhabibian No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran