fa بررسی تاثیر تغییر عملکرد ژن های مختلف بر واکنش گیاهان به اتیلن (C2H4) در شرایط in vitro در آرابیدوپسیس تالیانا تنظیم کننده های رشد گیاهی Plant Growth Regulators پژوهشي Research <!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--> <p style="margin-bottom: 0.0001pt text-align: justify line-height: normal" dir="rtl" class="MsoNormal"><!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--></p><p style="margin-bottom: 0.0001pt text-align: justify line-height: normal" dir="rtl" class="MsoNormal"><!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> </p><p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt text-align: justify line-height: normal"><!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--></p><p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt text-align: justify line-height: normal"><!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> <p style="margin-bottom: 0.0001pt text-align: justify line-height: normal" dir="rtl" class="MsoNormal"><!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--></p></p><p style="margin-bottom: 0.0001pt text-align: justify line-height: normal" dir="rtl" class="MsoNormal"><!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> <p style="margin-bottom: 0.0001pt text-align: justify line-height: normal" dir="rtl" class="MsoNormal"><span lang="FA" style="font-family: "B Lotus"">اتیلن، اگر چه یک ملکول ساده است، از جمله فاکتورهای مهم و موثر بر رشد و نمو گیاهان محسوب می شود. واکنش سه­گانه درگیاهچه­های اتیوله آرابیدپسیس به اتیلن </span><span dir="ltr"></span><span dir="ltr"></span><span style="font-family: "Times New Roman"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>(C₂H₄)</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> بطور گسترده ای جهت بررسی فعالیت بیوسنتز، درک و سیگنالینگ اتیلن و همچنین جداسازی موتانت­های مرتبط با این رخدادها در اتیلن استفاده می­شود. در این تحقیق گیاهان مختلف موتانت که هر یک حاوی موتاسیونی اختصاصی در یکی از ژن های مرتبط با سنتز هورمونهای<span class="msoIns"><ins datetime="2014-01-22T18:11" cite="mailto:office">ی</ins></span> مانند اتیلن</span><span lang="FA" style="font-size: 12pt font-family: "B Lotus"">(</span><i><span style="font-family: "Times New Roman"" dir="ltr">ein2</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> و</span><i><span style="font-family: "Times New Roman"" dir="ltr">edr1</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>)، جاسمونیک اسید(</span><i><span style="font-family: "Times New Roman"" dir="ltr">jar1</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>) و </span><span lang="FA" style="font-family: "B Lotus"">سالسیلیک اسید (</span><i><span style="font-family: "Times New Roman"" dir="ltr">pad4</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> و</span></i><span dir="ltr"></span><span dir="ltr"></span><i><span style="font-family: "Times New Roman"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> NahG</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>) در آرابیدوپسیس بودند جهت بررسی تاثیر تغییر عملکرد ژن های </span><i><span style="font-family: "Times New Roman"" dir="ltr">EIN2</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span lang="FA" style="font-family: Arial"><span dir="rtl"></span><span dir="rtl"></span>٬</span></i><i><span lang="FA" style="font-family: "B Lotus""> </span></i><i><span style="font-family: "Times New Roman"" dir="ltr">EDR1</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span lang="FA" style="font-family: Arial"><span dir="rtl"></span><span dir="rtl"></span>٬</span></i><i><span style="font-family: "Times New Roman"" dir="ltr">JAR1 </span></i><span dir="rtl"></span><span dir="rtl"></span><i><span lang="FA" style="font-family: Arial"><span dir="rtl"></span><span dir="rtl"></span>٬</span></i><i><span style="font-family: "Times New Roman"" dir="ltr">PAD4</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> و </span></i><i><span style="font-family: "Times New Roman"" dir="ltr">NahG</span></i><span style="font-family: "Times New Roman"" dir="ltr"> </span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span><span> </span>بر واکنش گیاهان به اتیلن مورد بررسی قرار گرفت. جهت انجام بررسی فوق از روش<span> </span>ارزیابی واکنش سه­گانه (</span><span style="font-family: "Times New Roman"" dir="ltr">Ethylene Triple Response</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>) برای ارزیابی گیاهان موتانت </span><i><span style="font-family: "Times New Roman"" dir="ltr">ein2</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>، </span><i><span style="font-family: "Times New Roman"" dir="ltr">jar1</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>، </span><i><span style="font-family: "Times New Roman"" dir="ltr">NahG</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>، </span><i><span style="font-family: "Times New Roman"" dir="ltr">pad4</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> و </span><i><span style="font-family: "Times New Roman"" dir="ltr">edr1</span></i><span dir="rtl"></span><span dir="rtl"></span><span style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">مورد توجه قرار گرفت. لذا بذور گیاهان فوق در محیط </span></span><span style="font-family: "Times New Roman"" dir="ltr">MS</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> شامل غلظت­های مختلف </span><span style="font-family: "Times New Roman"" dir="ltr">ACC</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> </span><span dir="ltr"></span><span dir="ltr"></span><span style="font-family: "Times New Roman"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>(1-aminocyclopropane-1-carboxylic acid)</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">برای مدت 5 روز در شرایط تاریکی مطلق نگهداری و سپس صفات سه­گانه چرخش قلاب راسی</span></span><span lang="FA" style="font-family: "Times New Roman"">٬</span><span lang="FA" style="font-family: "B Lotus""> طول هیپوکوتیل و طول ریشه­چه گیاهچه های موتانت مورد بررسی و تجزیه و تحلیل آماری قرار گرفت و با شاهد والدینی (</span><span style="font-family: "Times New Roman"" dir="ltr">Col-0</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>) مقایسه شد. بر اساس نتایج بدست­آمده، هیچ­کدام از موتانت­های مورد بررسی در دو گروه (</span><span style="font-size: 10pt font-family: "Times New Roman"" dir="ltr">Ethylene overexpression</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>)</span><span lang="FA" style="font-family: "B Lotus""> </span><i><span style="font-family: "Times New Roman"" dir="ltr">eto</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> </span></i><span lang="FA" style="font-family: "B Lotus"">و</span><span lang="FA" style="font-size: 12pt font-family: "B Lotus"">(</span><span style="font-size: 10pt font-family: "Times New Roman"" dir="ltr">hook less</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span>)</span><span lang="FA" style="font-family: "B Lotus""> </span><i><span style="font-family: "Times New Roman"" dir="ltr">hls</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> </span></i><span lang="FA" style="font-family: "B Lotus"">قرار نمی­گیرند. در حالیکه کلیه موتانت ها دارای تفاوتی معنی­دار با ژنوتیپ مادری در صفات 3 گانه واکنش به اتیلن بودند و فقط ژنوتیپ </span><i><span style="font-family: "Times New Roman"" dir="ltr">pad4</span></i><span dir="rtl"></span><span dir="rtl"></span><i><span style="font-family: "B Lotus""><span dir="rtl"></span><span dir="rtl"></span> </span></i><span lang="FA" style="font-family: "B Lotus"">از نظر طول هیپوکوتیل و ریشه­چه با ژنوتیپ مادری خود تفاوتی نداشت. لذا وقوع موتاسیون در ژن های موثر بر سنتز دیگر هورمون ها به نحو قابل توجهی میتواند بر واکنش گیاهان به اتیلن تغییر ایجاد نماید. همچنین بررسی واکنش سه­گانه در گیاهچه­های اتیوله موتانت میتواند بعنوان یک شاخص مهم و قابل توجهی برای ارزیابی واکنش گیاهان بالغ به اتیلن نیز محسوب گردد.</span></p> <span lang="FA" style="font-family: "B Lotus""> </span></p><p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt text-align: justify line-height: normal"> <span lang="FA"> </span></p> <span lang="FA"> </span><p></p> <!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> <!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--> <p class="MsoNormal" style="margin-bottom: 0.0001pt text-align: justify line-height: normal direction: ltr unicode-bidi: embed"><span><!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--></span></p><p class="MsoNormal" style="margin-bottom: 0.0001pt text-align: justify line-height: normal direction: ltr unicode-bidi: embed"><span><!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> <p style="margin-bottom: 0.0001pt text-align: justify line-height: normal direction: ltr unicode-bidi: embed" class="MsoNormal"><span style="font-family: "Times New Roman"">Ethylene (C₂H₄), although is a simple molecule, play several important roles in plant growth and development. Triple response approach is a great possibility for evaluation of ethylene biosynthesis and signaling in <i>Arabidopsis thaliana</i>. In this research, by using the various mutant plants, we investigate function of several key genes including <i>EIN2</i>, <i>EDR1</i>, <i>JAR1</i>, <i>PAD4</i> and <i>NahG</i> on plant responses to ethylene. The <i>Arabidopsis</i> seedlings that had some defection in ethylene biosynthesis, perception or signaling may explicate some changes in triple response. Therefore, several mutants including<i> ein2</i></span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus"" dir="rtl"><span dir="rtl"></span><span dir="rtl"></span>، </span><i><span style="font-family: "Times New Roman"">jar1</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus"" dir="rtl"><span dir="rtl"></span><span dir="rtl"></span>، </span><i><span style="font-family: "Times New Roman"">NahG</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus"" dir="rtl"><span dir="rtl"></span><span dir="rtl"></span>، </span><i><span style="font-family: "Times New Roman"">pad4</span></i><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-family: "B Lotus"" dir="rtl"><span dir="rtl"></span><span dir="rtl"></span> ،</span><i><span style="font-family: "Times New Roman"">edr1</span></i><span style="font-family: "Times New Roman""> and their wild type plant<span class="msoIns"><ins datetime="2014-01-24T11:09" cite="mailto:office">,</ins></span> Col-0, have been employed. The seeds have been grown on MS medium consisting of several concentrations <span>(0, 1, 5, 10 and 20 mM) </span>of ACC (1-aminocyclopropane-1-carboxylic acid). The triple response assay performed on seedlings after incubation for 5 days in darkness. <span>According to the obtained results, non of the mutants fall into eto (Ethylene over expression) and his (Less hook) groups. Most of mutant plantlets displayed a significant difference with the wild type, Col-0, and only </span><i>pad4</i> showed the same response as the parental genotype, meaning that ethylene biosynthesis and signaling in <i>pad4</i> seedlings is probably the same as the wild type.<span> Therefore, mutation in key genes involving in various hormone biosynthesis can induce a different response to ethylene in plants. The triple response approach could be very useful manner to predict the responses of plants in mature stage.</span></span></p>  </span><!--stripped--> gte mso 9]><xm<illegal tag>l> <!--stripped--> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> <!--stripped--></p><p class="MsoNormal" style="margin-bottom: 0.0001pt text-align: justify line-height: normal direction: ltr unicode-bidi: embed"><!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> </p><p class="MsoNormal" style="margin-bottom: 0.0001pt text-align: justify line-height: normal direction: ltr unicode-bidi: embed"><span><br></span><span><span> </span></span></p> <p></p> <!--stripped--> gte mso 9]><xm<illegal tag>l> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> <!--stripped--><!--stripped--> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:10.0pt font-family:"Times New Roman" mso-ansi-language:#0400 mso-fareast-language:#0400 mso-bidi-language:#0400} </style> <!--stripped--> اتیلن, ACC, گیاهچه اتیوله, واکنش سه گانه, موتانت ها Ethylene, ACC, Etiolate plantlets, Mutants, Triple response. 69 82 http://jispp.iut.ac.ir/browse.php?a_code=A-10-285-1&slc_lang=fa&sid=1 Hamideh Taheri سیده حمیده طاهری hamide.taheri2@gmail.com No Guilan University دانشگاه گیلان Reza Shirzadian-Khorramabad رضا شیرزادیان خرم آباد R.shirzadian@gmail.com Yes Guilan University دانشگاه گیلان Ali Alami علی اعلمی ali_aalami@yahoo.com No Guilan University دانشگاه گیلان Habibolah Samizadeh حبیب ا... سمیع زاده hsamizadeh@yahoo.com No Guilan University دانشگاه گیلان