fa مطالعه تغییرات آنزیمی و ارزیابی بیان برخی ژن‌های مهم در تحمل به شوری در چهار رقم از برنج تحت شرایط هیدروپونیک تنش شوري Salt Stress پژوهشي Research <span style="font-size:12pt"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شوری، یکی از مهم&shy;ترین عوامل محدودکننده رشد </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">و</span></span></b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> تولید برنج در شرایط خشک و نیمه&shy;خشک جهان است. به</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">منظور</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بررسی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">تأثیر</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">تنش</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شوری</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بر خصوصیات بیوشیمیایی و بیان برخی ژن‌های القایی</span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">در مرحله گیاهچه‌ای</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">برنج، آزمایشی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">به</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">صورت کرت&shy;های خرد شده با</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">طرح</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">پایه</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بلوک&shy;های</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">کامل</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">تصادفی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">با چهار</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">تکرار</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">انجام</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شد.</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">فاکتور اصلی شامل پنج</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">سطح</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">صفر</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">(شاهد)، 50، 100، 150 و 200 میلی&shy;مولار از</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">کلرید</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">سدیم</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">برای</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">ایجاد</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">تنش</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شوری بود. سطوح شوری به</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">همراه</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">محلول</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">غذایی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">یوشیدا</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">در</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">یک</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">سیستم هیدروپونیک</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">اعمال</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شد.</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> فاکتور فرعی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شامل</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">چهار</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">رقم</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">(کشوری، شیرودی، طارم و کوهسار)</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">برنج</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بود</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">. </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">در این پژوهش شاخص سطح اکسیداسیون سلولی </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">(</span></span></b><b><span dir="LTR" style="font-size:9.0pt">TBARM</span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">)، فعالیت‌های آنزیمی و بیان برخی ژن‌های مهم پاد اکسیدان شامل کاتالاز، سوپراکسید دیسموتاز، آسکوربیک پراکسیداز و فاکتور رونویسی </span></span></b><b><i><span dir="LTR" style="font-size:9.0pt">TCTP</span></i></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">مورد بررسی قرار گرفت.</span></span></b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> نتایج</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">حاصل</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">از تجزیه</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">واریانس</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">داده‌ها نشان</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">داد</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">که</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">اختلاف</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بین</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">ارقام</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">و</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">سطوح</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">شوری</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">برای</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">کلیه</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">صفات</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">مورد</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بررسی</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">در</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">سطح</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">احتمال</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">یک</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">درصد معنی‌دار</span></span></b><b> </b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">بود</span></span></b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">. </span></span></b><b><span lang="AR-SA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">همچنین </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:">با افزایش سطح شوری، میزان فعالیت آنزیم‌های مورد مطالعه و بیان ژن‌های کاتالاز، سوپراکسید دیسموتاز، آسکوربیک پراکسیداز و </span></span></b><b><i><span dir="LTR" style="font-size:9.0pt">TCTP</span></i></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> افزایش یافت.</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span b="" lotus="" style="font-family:"> با توجه به نتایج به&shy;دست آمده رقم‌‌های شیرودی و کوهسار به عنوان رقم&shy;های متحمل به شوری شناسایی شدند.</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Lotus""></span></span></b></span></span></span></span></span> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:11.0pt">Salinity is one of the most important growth limiting factors and rice production in arid and semi-arid regions of the world. In order to investigate the effect of salinity stress on biochemical characteristics and the expression of some inducible genes in the seedling stage of rice, an experiment in the form of chopped plots with a basic design of randomized complete blocks were performed with four replications. The main factor included five levels of zero (control), 50, 100, 150 and 200 mM sodium chloride to create salinity stress. Salinity levels were applied along with Yoshida&#39;s nutrient solution in a hydroponic system. The sub-factor included four varieties of rice (Kashuri, Shiroodi, Tarem and Kohsar). In this research, the cellular oxidation level index (TBARM), enzyme activities and expression of some important antioxidant genes, including catalase, superoxide dismutase, ascorbic peroxidase, and the TCTP transcription factor, were investigated. The results of the variance analysis of the data showed that the difference between cultivars and salinity levels for all studied traits was significant at the probability level of 1%. Also, by increasing the salinity level, the activity of the studied enzymes and the expression of catalase, superoxide dismutase, ascorbic peroxidase and TCTP genes increased. According to the obtained results, Shiroudi and Kohsar cultivars were identified as salinity tolerant cultivars.</span></span></span> برنج, بیان ژن, تنش شوری, صفات بیوشیمیایی Biochemical traits, Gene expression, Rice, Salinity stress 357 368 http://jispp.iut.ac.ir/browse.php?a_code=A-10-1316-4&slc_lang=fa&sid=1 saeed navabpour سعید نواب پور 100319475328460034090 100319475328460034090 Yes Department of Plant Breeding and Biotechnology, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran گروه اصلاح نباتات و بیوتکنولوژی دانشگاه منابع طبیعی و علوم کشاورزی گرگان، ایران sanaz ramezanpour ساناز رمضانپور horeyeh.1372@gmail.com 100319475328460034091 100319475328460034091 No Department of Plant Breeding and Biotechnology, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran گروه اصلاح نباتات و بیوتکنولوژی دانشگاه منابع طبیعی و علوم کشاورزی گرگان، ایران ahad yamchi احد یامچی 100319475328460034092 100319475328460034092 No Department of Plant Breeding and Biotechnology, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran گروه اصلاح نباتات و بیوتکنولوژی دانشگاه منابع طبیعی و علوم کشاورزی گرگان، ایران daryosh abadi almas داریوش عبادی الماس 100319475328460034093 100319475328460034093 No Department of Plant Breeding and Biotechnology, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran گروه اصلاح نباتات و بیوتکنولوژی دانشگاه منابع طبیعی و علوم کشاورزی گرگان، ایران