en Selenium elicitation and increase of secondary metabolites production by suspension-cultured Astragalus verus cells سایر موارد others پژوهشي Research <pre dir="RTL" style="text-align: left;"> <span style="font-size:12pt"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"><b><span style="font-size:9.0pt">Secondary metabolites with low molecular weight and high economic, pharmaceutical and industrial values play an important role in protecting plants against biotic and abiotic stresses. Astragalus is a medicinal herb, rich in secondary metabolites. Selenium (Se) is an essential element for humans, animals, many bacteria, as well as a beneficial element for plants. The plants response to selenium is depending on their species and the Se supply concentration. In the present study, calli were established from the seeds of <i>Astragalus verus</i> and suspension cultures were established from the calli. The cells were treated with different concentrations of sodium selenate (0, 0.5, 2.5, 12.5, and 62.5 &mu;M) for 1 week at their logarithmic growth phase (based on growth curve). Cell growth and viability significantly decreased by selenium at concentrations higher 2.5 &mu;M. In comparison with control group, significant increases in Abscisic acid and Salicylic acid were observed at higher Se concentrations, whereas methyl jasmonate significantly increased at lower Se supply. At 62.5 &mu;M Se supply the contents of alkaloids, phlobaphene, steroidal and triterpenoid saponins, and tannin respectively increased by 5.1, 1.51, 1.62, 1.75, and 2.04 times of control. The results provides a practical method for remarkable stimulation of natural compounds of Astargalus by Se.</span></b></span></span> </pre> <pre dir="RTL" style="text-align: left;"> </pre> <span style="font-size:12pt"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"><b><span style="font-size:9.0pt">Secondary metabolites with low molecular weight and high economic, pharmaceutical and industrial values play an important role in protecting plants against biotic and abiotic stresses. Astragalus is a medicinal herb, rich in secondary metabolites. Selenium (Se) is an essential element for humans, animals, many bacteria, as well as a beneficial element for plants. The plants response to selenium is depending on their species and the Se supply concentration. In the present study, calli were established from the seeds of <i>Astragalus verus</i> and suspension cultures were established from the calli. The cells were treated with different concentrations of sodium selenate (0, 0.5, 2.5, 12.5, and 62.5 &mu;M) for 1 week at their logarithmic growth phase (based on growth curve). Cell growth and viability significantly decreased by selenium at concentrations higher 2.5 &mu;M. In comparison with control group, significant increases in Abscisic acid and Salicylic acid were observed at higher Se concentrations, whereas methyl jasmonate significantly increased at lower Se supply. At 62.5 &mu;M Se supply the contents of alkaloids, phlobaphene, steroidal and triterpenoid saponins, and tannin respectively increased by 5.1, 1.51, 1.62, 1.75, and 2.04 times of control. The results provides a practical method for remarkable stimulation of natural compounds of Astargalus by Se.</span></b></span></span> Alkaloids, Astragalus verus, Phytohormones, Phlobaphene, Saponin, Sodium selenate Alkaloids, Astragalus verus, Phytohormones, Phlobaphene, Saponin, Sodium selenate 11 18 http://jispp.iut.ac.ir/browse.php?a_code=A-10-99-9&slc_lang=en&sid=1 Negar Maassoumi Negar Maassoumi No Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB141115-154, Tehran, Iran Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB141115-154, Tehran, Iran Faezeh Ghanati Faezeh Ghanati 0000-0002-2901-0152 Yes Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB141115-154, Tehran, Iran Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB141115-154, Tehran, Iran Hassan Ahmadi Gavlighi Hassan Ahmadi Gavlighi No Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University (TMU), POB14115-336, Tehran, Iran Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University (TMU), POB14115-336, Tehran, Iran