en In vito organogenesis origin site detection with explants anatomical study of damask rose (Rosa damascena Mill.) بیوتکنولوژی biotechnology پژوهشي Research <div style="text-align: left;"><span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt">Damask rose is </span></i></b><b><span style="font-size:10.0pt">one of the aromatic and medicinal plants belonging to the Rosacea family whose essential oil has nutritional and economic value. The purpose of this research was to determine the location of the hormones effects in organ tissues for the formation of calluses, buds and roots. For this, we have studied hormone effects on the callus, shoot, root formation, and anatomical alternate. We found IAA and 2, 4-D with BAP were affected by callus, shoot, and root production from damask shoot explants. Regeneration organs were undifferentiated to callus after two weeks. To avoid this problem, we suggest that it is better to subculture explants in a new medium with fewer hormones. The site of callus, shoot, and root formation was the node on the explant. This explant node had a lateral meristem. The anatomical study of explants showed that the origin of callus, shoot, and root formation is cambium parenchymal cells. </span></b></span></span></div> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt">Damask rose is </span></i></b><b><span style="font-size:10.0pt">one of the aromatic and medicinal plants belonging to the Rosacea family whose essential oil has nutritional and economic value. The purpose of this research was to determine the location of the hormones effects in organ tissues for the formation of calluses, buds and roots. For this, we have studied hormone effects on the callus, shoot, root formation, and anatomical alternate. We found IAA and 2, 4-D with BAP were affected by callus, shoot, and root production from damask shoot explants. Regeneration organs were undifferentiated to callus after two weeks. To avoid this problem, we suggest that it is better to subculture explants in a new medium with fewer hormones. The site of callus, shoot, and root formation was the node on the explant. This explant node had a lateral meristem. The anatomical study of explants showed that the origin of callus, shoot, and root formation is cambium parenchymal cells. </span></b></span></span> Cytokinin, Auxin, Shoot, Root, Cambium, Meristem Cytokinin, Auxin, Shoot, Root, Cambium, Meristem 41 46 http://jispp.iut.ac.ir/browse.php?a_code=A-10-718-5&slc_lang=en&sid=1 Milad Razaji Milad Razaji lactaa_milk@yahoo.com No Department of Plant Biology, Saveh Branch, Islamic Azad University, Saveh, Iran Department of Plant Biology, Saveh Branch, Islamic Azad University, Saveh, Iran Babak Delnavaz Hashemloian Babak Delnavaz Hashemloian hashemloian1343@gmail.com No Department of Plant Biology, Saveh Branch, Islamic Azad University, Saveh, Iran Department of Plant Biology, Saveh Branch, Islamic Azad University, Saveh, Iran Azra Ataei Azimi Azra Ataei Azimi baharana1395@gmail.com Yes Department of Plant Biology, Tehran Shargh Branch, Islamic Azad University, Tehran, Iran Department of Plant Biology, Tehran Shargh Branch, Islamic Azad University, Tehran, Iran Mojtba Yosefirad Mojtba Yosefirad ymojtab@gmail.com No Department of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran Department of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran Reza Rezakhanluo Reza Rezakhanluo No Department of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran Department of Agriculture, Saveh Branch, Islamic Azad University, Saveh, Iran