Plant Process and Function

en Some biochemical compounds in male and female plants of Ephedra intermedia Schrenket Some biochemical compounds in male and female plants of Ephedra intermedia Schrenket سایر موارد others پژوهشي Research Ephedra intermedia is a dioecious plant of the Ephedraceae family whose male and female plants are quite similar in shape, but their biochemical differences are not known. The subject was the TLC and GC-MS study of fatty acids, phenols, essential oils and alkaloids in male and female shoots of E. intermedia. Male and female shoots were collected from Markazi province in Iran, in spring. Alcoholic extract was obtained by mixing shoot powder with ethanol at 60 °C. Phenols, fatty acids and essential oil were separated from the alcoholic extract with ether and alkaloids with chloroform. Male and female ether extracts contained fatty acids, which appeared male to be a good source of linolenic acid. Kind and amount of organic acids and phenolic of two sexes were different. Large quantity of caprylic acid isoamyl ester was present only in female. 2, 6-Di-Tert-4-Methylphenol was much higher in both sexes than the other compounds. A large number of essential oil compounds were found in the etheric extract of both sexes. The similarity of essential oil compounds between male and female plants was 32.4%. Total essential oils in female and male plants were 9.63 and 11.79 mgg-1DW. Males and females had a large number of alkaloids, but there was no ephedrine and pseudoephedrine. Epherodxan and phendimetrazine in both sexes, ecgonine and aminopyrimidine in the male, and ergotamine and theacrin in the female, were six important medicinal alkaloids. Both sexes of E. intermedia were rich in bioactive compounds, but there was no ephedrine or pseudoephedrine. Ephedra intermedia is a dioecious plant of the Ephedraceae family whose male and female plants are quite similar in shape, but their biochemical differences are not known. The subject was the TLC and GC-MS study of fatty acids, phenols, essential oils and alkaloids in male and female shoots of E. intermedia. Male and female shoots were collected from Markazi province in Iran, in spring. Alcoholic extract was obtained by mixing shoot powder with ethanol at 60 °C. Phenols, fatty acids and essential oil were separated from the alcoholic extract with ether and alkaloids with chloroform. Male and female ether extracts contained fatty acids, which appeared male to be a good source of linolenic acid. Kind and amount of organic acids and phenolic of two sexes were different. Large quantity of caprylic acid isoamyl ester was present only in female. 2, 6-Di-Tert-4-Methylphenol was much higher in both sexes than the other compounds. A large number of essential oil compounds were found in the etheric extract of both sexes. The similarity of essential oil compounds between male and female plants was 32.4%. Total essential oils in female and male plants were 9.63 and 11.79 mgg-1DW. Males and females had a large number of alkaloids, but there was no ephedrine and pseudoephedrine. Epherodxan and phendimetrazine in both sexes, ecgonine and aminopyrimidine in the male, and ergotamine and theacrin in the female, were six important medicinal alkaloids. Both sexes of E. intermedia were rich in bioactive compounds, but there was no ephedrine or pseudoephedrine. Ephedra intermedia, Male, Female, Alkaloid, Phenol, Fatty acid Ephedra intermedia, Male, Female, Alkaloid, Phenol, Fatty acid 11 18 http://jispp.iut.ac.ir/browse.php?a_code=A-10-1472-1&slc_lang=en&sid=1 Maryam Ahmadi Maryam Ahmadi ahmadimaryam257@gmail.com No Department of Plant Biology, Agriculture Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran. Department of Plant Biology, Agriculture Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran. Babak Delnavaz hashemloian Babak Delnavaz hashemloian hashemloian1343@gmail.com 0000-0001-7992-6487 Yes Department of Plant Biology, Agriculture Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran. Department of Plant Biology, Agriculture Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran. Azra Ataei Azimi Azra Ataei Azimi baharana1395@gmail.com 0000-0002-8070-482X No Department of Plant Biology, Agriculture Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran. Department of Plant Biology, Agriculture Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran. Pejman Mordi Pejman Mordi pjmoradi@gmail.co No Department of horticulture, Agriculture faculty, Saveh branch, Islamic Azad University, Saveh, Iran Department of horticulture, Agriculture faculty, Saveh branch, Islamic Azad University, Saveh, Iran