Plant Process and Function
فرآیند و کارکرد گیاهی
Journal of Plant Process and Function
Agriculture
http://jispp.iut.ac.ir
1
admin
2322-2727
2383-3688
10.22034
fa
jalali
1400
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1
gregorian
2021
7
1
10
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en
Epichloë Endophyte Modifies Antioxidative Defense and Aquaporin Genes Expression in Response to Ni Contamination in Lolium perenne
Epichloë Endophyte Modifies Antioxidative Defense and Aquaporin Genes Expression in Response to Ni Contamination in Lolium perenne
تنش فلزات سنگین
Toxic ions stress
پژوهشي
Research
The aim of this study was to determine the impacts of endophyte (E) inoculation on Nickel tolerance of the <em>Lolium prenne</em> by measuring the physiological and biochemical traits in two populations of ryegrass consisting both (E-) and (E+) plants cultivated under Ni stress. The plants were grown in a Ni polluted soil at 0, 30, 90 and 180 mg Ni kg <sup>-1</sup>. The present research showed that the activity of antioxidant enzymes increased in Ni treated plants in contrast to control plants; whereas the activity of glutathione reductase enzyme decreased. Also, endophyte infection increased the activity of some antioxidant enzymes but decreased the activity of SOD enzyme. Upregulation of aquaporin gene (<em>LpTIP1;1</em>) was shown in population 1 E+ plants at all treatments and 30 ppm Ni in E- plants, whereas, the expression of <em>LpTIP1;2</em> in population 1 E- plants increased and no significant difference was shown for E+ plants. After 3 months from treating plants, considerable reduction in shoot biomass of E+ and E- plants was observed in 180 ppm Ni and endophyte inoculation decreased the biomass of plants. The shoot water content of E- plants was greater than E+ counterparts. A significant increase of Ni concentration of roots and shoots was also observed under Ni stress and the Ni concentration of E+ plants was 88.7% and 12.7% greater in shoots and roots, respectively, than in E- plants. Overall, results suggest that variation in response to Ni stress in E+ and E- ryegrass populations may aid survival of the grass under stress conditions.
Keywords: Aquaporin, Endophyte, Epichloe festuca, Nickel, Reygrass
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http://jispp.iut.ac.ir/browse.php?a_code=A-10-57-11&slc_lang=en&sid=1
Maryam
Salehi
Maryam
Salehi
100319475328460024151
100319475328460024151
No
Department of Plant Sciences, Faculty of Sciences, Shahrekord University, Iran
Department of Plant Sciences, Faculty of Sciences, Shahrekord University, Iran
Leila
shabani
Leila
shabani
lshabani@gmail.com
100319475328460024152
100319475328460024152
Yes
1Department of Plant Sciences, Faculty of Sciences, Shahrekord University, Iran 2Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran
1Department of Plant Sciences, Faculty of Sciences, Shahrekord University, Iran 2Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran
Mohammad reza
Sabzalian
mohammad reza
Sabzalian
100319475328460024153
100319475328460024153
No
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111