en سایر موارد others پژوهشي Research <strong>The aim of the present research is to investigate the profile of amino acid of <em>Chlorella vulgaris</em> supplemented with some plant amino acid and oil extracts. </strong><strong>The bioactive oil and amino acid from four Iranian medicinal plants, namely <em>Oliveria decumbens</em>, <em>Thymus kotschyanus</em>, <em>Trachyspermum ammi</em>, and <em>Zataria multiflora</em> were obtained. </strong><strong>The LC-MS / MS analysis demonstrated that the major amino acids component of <em>Chlorella vulgaris</em> were alanine (30.4 mg/g), glycine (23.2 g/g), glutamic acid (22.7 mg/g), aspartic acid (21.1 mg/g), arginine (20.9 mg/g), leucine (18.9 mg/g), lysine (16.5 mg/g), phenylalanine (14.0 mg/), serine (13.0 mg/g), asparagine (12.7 mg/g), valine (12.6 mg/g),&nbsp; isoleucine (11.5 mg/g), and tyrosine (11.0 mg/g). </strong><strong>The major fatty acids of the plants considered are </strong><strong>9, 12, 15-octadecatrienoic acid</strong><strong>, </strong><strong>9, 12-octadecadienoic acid</strong><strong>, </strong><strong>9-octadecenoic acid</strong><strong>, and </strong><strong>hexadecanoic acid</strong> <strong>but with different concentrations.</strong><strong> The major non-fat components (lipophilic monoterpenoid) detected in the oils were thymol and carvacrol with different concentrations. Vegetable oils mainly composed of polyunsaturated fatty acids and reduced amino acid contents in <em>Chlorella vulgaris</em>. </strong><strong>The main amino acids from plant-derived protein hydrolysate were aspartic acid, glycine, glutamic acid, leucine, alanine, lysine, arginine, serine, phenylalanine, valine, proline, and histidine but with a different level. </strong><strong>&nbsp;Vegetable amino acids mainly composed of functional amino acids and significantly enhanced amino acid production in <em>Chlorella vulgaris</em>. <em>Zataria multiflora</em>, <em>Trachyspermum ammi</em> and <em>Thymus kotschyanus</em> amino acid supplementations as nitrogen and carbon sources also exhibited the same and better effects on the amino acid profile compared to the <em>Oliveria decumbens</em> amino acid supplementation.</strong> Chlorella vulgaris, Amino acids, Fatty acid Supplementation, Amino acid Supplementation 55 62 http://jispp.iut.ac.ir/browse.php?a_code=A-10-1423-1&slc_lang=en&sid=1 Gholamreza Kavoosi ghkavoosi@shirazu.ac.ir Yes Shiraz University Roghayeh Siahbalaei m.siahbalaei@shirazu.ac.ir No Shiraz University