Plant Process and Function
فرآیند و کارکرد گیاهی
Journal of Plant Process and Function
Agriculture
http://jispp.iut.ac.ir
1
admin
2322-2727
2383-3688
10.22034
fa
jalali
1399
5
1
gregorian
2020
8
1
9
37
online
1
fulltext
en
An Assessment of Oxidative Stress and Antioxidant System Activity in Alfalfa Plants Treated with Different Forms of Mineral Arsenic
تنش فلزات سنگین
Toxic ions stress
پژوهشي
Research
<strong>Arsenic (As), a metalloid and unnecessary element, is highly toxic to living organisms. It can be accumulated in the environment and enter the food chains through uptake by plants. The present study was designed to assess the response of antioxidant defense system of alfalfa to As (As<sup>0</sup>, As<sup>3+</sup> and As<sup>5+</sup>) induced oxidative stress at four rates (0, 100, 200, and 300 </strong><strong>µM</strong>) <strong>as a completely randomized design with three replications in the laboratory conditions. Tukey test was used for data analysis. </strong><strong>The 90-day seedlings after harvesting, were </strong><strong>used</strong><strong> to evaluate MDA and H<sub>2</sub>O<sub>2 </sub>contents, antioxidant system, including SOD, POD, APX, CAT activities, contents of phenol, flavonoids and anthocyanin. According to the results, root and shoot H<sub>2</sub>O<sub>2</sub> contents of As<sup>3+</sup>-treated plants, were the highest at the rate of 300 µM and the MDA contents were the highest at the rates of 200 and 300 µM in root and shoot, respectively. However, in 200 µM of As<sup>0</sup>-treated plants, the MDA content showed a significant decline (P≤ 0.05). </strong><strong>The antioxidant responses varied with </strong><strong>different treatments</strong><strong>. Increased free radical scavenging activity was only observed at lower rates of </strong><strong>As<sup>5+</sup></strong><strong> in the shoot. Consequently it can be concluded that all of three forms of As, induced oxidative stress, which </strong><strong>As<sup>3+</sup></strong><strong> (300 </strong><strong>µM</strong><strong>) was more toxic than </strong><strong>As<sup>0</sup></strong><strong> and </strong><strong>As<sup>5+</sup>.</strong> <strong>Nonetheless, the alfalfa plant mitigated As-induced oxidative stress by manipulating the activity of their antioxidant system through</strong> <strong>increasing</strong> <strong>contents of total phenol, flavonoids, anthocyanin and changing antioxidant enzymes activities, in different types and rates of As.</strong><br>
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<strong><span dir="RTL"></span></strong>
oxidative stress, arsenic, enzymatic antioxidant, non-enzymatic antioxidant, Medicago sativa.
13
26
http://jispp.iut.ac.ir/browse.php?a_code=A-10-69-5&slc_lang=en&sid=1
Farnoosh
Zargari
farnooshzargari@yahoo.com
100319475328460021879
100319475328460021879
No
Urmia University
Latifeh
Pourakbar
lpourakbar@yahoo.com
100319475328460021880
100319475328460021880
Yes
Urmia University
Seyed Yahya
Salehi-Lisar
y_salehi@tabrizu.ac.ir
100319475328460021881
100319475328460021881
No
Tabriz University
Jafar
Razeghi
jafar_razeghi@tabrizu.ac.ir
100319475328460021882
100319475328460021882
No
Tabriz University
Rouhollah
Motafakker Azad
rmotafakker@tabrizu.ac.ir
100319475328460021883
100319475328460021883
No
Tabriz University