fa تأثیر اسیدهیومیک بر شاخص‌های جوانه‌زنی و آنزیم‌های آنتی‌اکسیدان بذر کلزا (Brassica napus L.) تحت تاثیر سمیت نانو ذره اکسید مس تنش فلزات سنگین Toxic ions stress پژوهشي Research <p dir="RTL" style="margin: 0in 0in 0pt; text-align: justify; line-height: normal; unicode-bidi: embed; direction: rtl;"><font size="3"><b><span b="" lang="FA" mso-bidi-language:="" style="color: black; font-family: ">با توجه به افزایش تولید نانو ذرات در سال‌های اخیر و کاربرد آن‌ها در برنامه‌های متنوع مانند پزشکی، تصویربرداری، کاتالیزور، نیمه‌رساناها، مواد آرایشی-بهداشتی، میکروالکترونیک، پوشش روی مواد مختلف و غیره، در حال حاضر نگرانی‌هایی در مورد خطر احتمالی و آلودگی محیط‌زیست به نانو ذرات در سطح جهان به وجود آمده است.</span></b><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""><font size="3"><font color="#000000">اثر سمیت نانو ذره اکسید مس </font><span style="color: black;">بر فعالیت </span></font><font color="#000000" size="3">برخی آنزیم‌های</font><span style="color: black;"><font size="3"> آنتی اکسیدانی، پروتئین</font></span></span></b><font size="3"><b><span b="" dir="LTR" lang="FA" mso-bidi-font-family:="" mso-bidi-language:="" new="" style="background: white; color: black; font-family: " times=""> </span></b><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""><font size="3"><font color="#000000">جوانه زنی</font><span style="color: black;"> گیاه کلزا و همچنین بررسی اثرات </span></font><font color="#000000" size="3">اسید هیومیک بر سمیت نانو ذره</font><span style="color: black;"><font size="3"> در محیط آزمایشگاه بود. آزمایشی در مرحله جوانه زنی گیاه کلزا (رقم اکاپی) به‌منظور بررسی تاثیر نانو ذره اکسید مس در</font></span></span></b><font size="3"><b><span b="" color="#000000" font="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">۹</span><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">۰، ۲۵۰، ۵۰۰، ۷۵۰، ۱۰۰۰، ۱۲۵۰،</span><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: black; font-family: " times="">۱</span><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: black; font-family: " times=""> </span></b><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""><font color="#000000"> </font></span></b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: black; font-family: " times=""> غلظت </span></b></b></font><b><span b="" font="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" size="3" span="" style="background: white; color: black; font-family: " times="">میلی گرم در لیتر، </span></b><font color="#000000"><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""> </span></b></font><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""><font color="#000000"> تکرار</font><span style="color: black;"> به‌ صورت</span></span></b><b><span b="" dir="LTR" lang="FA" mso-bidi-font-family:="" mso-bidi-language:="" new="" style="background: white; color: black; font-family: " times=""> </span></b><b><span .="" b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""><font color="#000000">فعالیت هرچهار آنزیم</font><span style="color: black;"> سوپراکسیددیسموتاز، آسکوربات پراکسیداز، کاتالاز و پراکسیداز به ترتیب</span></span></b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">00/5و ۱۹/۲و 00/2و ۳۵/۲</span><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: black; font-family: " times="">برابر افزایش ولی میزان پروتئین به میزان</span><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">2</span><b><span .="" b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">طول ریشه&shy;چه، طول ساقه&shy;چه</span></b><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">0/39</span><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">56/6</span><b><span .="" b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">۲۰۰۰<b> </b></span><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">ریشه&shy;چه، ساقه&shy;چه، وزن تر ریشه&shy;چه و ساقه&shy;چه </span></b><b><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">68/9و</span><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; color: red; font-family: " times="">0/3و 0/6و 50/5</span><span b="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="background: white; font-family: " times=""><font color="#000000">.</font></span></b><b><span lang=&quot;FA&quot; style=&quot;font-family: &quot; b=&quot;&quot; lotus&quot;;=&quot;&quot; mso-bidi-language:=&quot;&quot; fa;=&quot;&quot; mso-ascii-font-family:=&quot;&quot; &quot;times=&quot;&quot; new=&quot;&quot; roman&quot;;=&quot;&quot; mso-hansi-font-family:=&quot;&quot; roman&quot;;&quot;=&quot;&quot;><font color="#000000"> </font><span style="color: black;">می&shy;توان اظهار داشت که اسید هیومیک با افزایش فعالیت شبه هورمونی می&shy;تواند سبب افزایش فعالیت آنزیم آنتی‌اکسیدان و رشدگیاه گردد</span></b></b></b></font><b><b><span b="" dir="LTR" font-size:="" mso-bidi-font-family:="" mso-bidi-language:="" new="" style="color: black; font-family: " times="">.</span></b></b></font></p> <p style="margin: 0in 0in 0pt; text-align: justify; line-height: normal;"><font size="3"><span lang="EN" mso-ansi-language:="" mso-ascii-theme-font:="" mso-bidi-theme-font:="" mso-hansi-theme-font:="" new="" style="color: rgb(34, 34, 34); font-family: " times="">Due to the increasing production of nanoparticles in recent years and their applications in various applications such as medicine, imaging, catalysts, semiconductors, cosmetics, microelectronics, coatings on various materials, etc., </span><span lang="EN" mso-ansi-language:="" mso-ascii-theme-font:="" mso-bidi-theme-font:="" mso-hansi-theme-font:="" new="" style="color: red; font-family: " times="">there are </span><span lang="EN" mso-ansi-language:="" mso-ascii-theme-font:="" mso-bidi-theme-font:="" mso-hansi-theme-font:="" new="" style="color: rgb(34, 34, 34); font-family: " times="">currently concerns about the potential hazard and environmental pollution. Nanoparticles have been created globally.</span><span lang="EN" mso-ansi-language:="" mso-ascii-theme-font:="" mso-bidi-theme-font:="" mso-hansi-theme-font:="" new="" style="font-family: " times=""><font color="#000000"> The purpose of this study was to investigate the effect of copper oxide nanoparticle toxicity on the activity of some antioxidant enzymes, protein and germination indices of canola, and also to investigate the effects of humic acid on nanoparticle toxicity in laboratory environment. </font><span style="color: red;">An experiment </span><span style="color: rgb(34, 34, 34);">was conducted at the germination stage of rapeseed (Octopus cultivar) to investigate the effect of copper oxide nanoparticles in concentrations of 0, 250, 500, 750, 1000, 1250, 1500, 1750, 2000 mg per liter and humic acid in concentrations of 0, 100 mg / L, in 3 replicates, a factorial in a completely randomized design. The results showed that, with increasing concentrations of copper oxide nanoparticles, the levels of activity of all four superoxide dismutase enzymes, ascorbate peroxidase, catalase and peroxidase increased (5.00, 2.9, 2.00, 2.35), respectively, but the amount of protein 2 times lower. Germination indices (root length, stem length) also decreased (39.3, 56.6, 12.7), respectively.</span></span><span lang="EN" mso-ansi-language:="" style="color: rgb(34, 34, 34); font-family: "> </span><span lang="EN" mso-ansi-language:="" mso-ascii-theme-font:="" mso-bidi-theme-font:="" mso-hansi-theme-font:="" new="" style="color: rgb(34, 34, 34); font-family: " times="">The use of humic acid reduced the toxicity effects of copper oxide nanoparticles, especially at the level of 2000 mg / L, so that the root length, stem, root and shoot stomatal length (respectively) 68/9, 0/3, 0/6, 5/5) decreased compared to the control. It can be stated that humic acid can increase the activity of antioxidant enzymes and increase growth by increasing the activity of quasi-hormonal activity.</span></font></p> آلودگی, درصد نهایی جوانه زنی, فعالیت بیوشیمیایی, گیاهان روغنی, مواد آلی طبیعی, نانوذرات Contamination, Final Germination percentage, Biochemical activity, Oily plants, Natural organic matter, Nanoparticles 195 206 http://jispp.iut.ac.ir/browse.php?a_code=A-10-1083-1&slc_lang=fa&sid=1 افسانه یارمحمدی yarmohammadi1370@yahoo.com No دانشگاه رازی کرمانشاه محمود خرمی وفا khoramivafa@gmail.com Yes دانشگاه رازی کرمانشاه سعید جلالی هنرمند Sjhonarmand@yahoo.com No دانشگاه رازی کرمانشاه