TY - JOUR JF - jispp JO - VL - 7 IS - 26 PY - 2018 Y1 - 2018/11/01 TI - Effect of gibberellic acid and priming on germination and some constituents of Stevia (Stevia rebaudiana Bertoni) TT - اثر اسید جیبرلیک و پرایمینگ بر جوانه‌زنی و برخی از ترکیبات استویا (Stevia rebaudiana Bertoni) N2 - Considering low vigor of Stevia (Stevia rebaudiana Bertoni) seeds and difficulty of its germination, the purpose of this experiment was to evaluating the effect of different priming types on germination and seedling growth of Stevia in the presence of gibberellic acid. A factorial experiment was carried out based on a completely randomized design with three replications. Treatments consisted of different concentrations of gibberellic acid (G1=0, G2 =25 and G3=50 mg L-1) and seed priming as hydro, hormone (gibberellic acid, 0.2 mg L-1) and Osmo (3% potassium nitrate) for 18 hours. The essential oil constituents were measured by gas chromatography (GC). The activity of hydrolytic and antioxidant enzymes in seedlings also were measured. The highest germination percentage (67.26 %) observed in the hormone priming and the concentration of 50 mg L-1 of gibberellic acid. Osmopriming had the highest germination rate. The highest levels of SOD activity and MDA content observed in osmopriming and catalase and peroxidase achieved in hydro priming without application of gibberellic acid. The highest level of alpha-amylase enzyme activity was related to hormone priming with 50 mg L-1 gibberellic acid. The highest levels of alphacadinol, methyl salicylate and Safranal and also carbohydrates and protein observed in hormone priming and 50 mg L-1 gibberellic acid. In conclusion, to achieve the highest essential oil contents in Stevia using hormone priming and application of 50 mg L-1 gibberellic acid was suitable. SP - 199 EP - 208 AU - Sedghi, Mohammad AU - Sheikhnavaz Jahed, Paris AU - Seyed Sharifi, Raouf AD - University of Mohaghegh Ardabili KW - Anti-oxidant enzymes KW - Germination KW - seed pre-treatment KW - gibberellic acid KW - alpha-amylase UR - http://jispp.iut.ac.ir/article-1-757-en.html ER -