RT - Journal Article T1 - ​Surveying the inhibition of mevanolate / acetate (MVA) pathway in lipid production in green algae of Dunaliella salina and Dunaliella bardawil by using Lovastatin JF - jispp YR - 2018 JO - jispp VO - 7 IS - 26 UR - http://jispp.iut.ac.ir/article-1-1037-en.html SP - 277 EP - 285 K1 - D. bardawil K1 - D. salina K1 - lipid K1 - lovastatin K1 - mevanolate AB - Surveying the inhibition of mevanolate / acetate (MVA) pathway in lipid production in green algae of Dunaliella salina and Dunaliella bardawil by using Lovastatin Z and * Department of Biology, Faculty of Science, University of Isfahan Abstract Many of microalgae are rich in lipid and are very much considered due to the limitations of nutritional needs and rapid growth. Since the lovastatin inhibits the pathway of mevanolate (the pathway of beta-carotene biosynthesis) and the beta-carotene is a terpenic compound and is a simple lipid types, in this study, the inhibitory effect of lovastatin on two microalgae Dunaliella salina and Dunaliella bardawil was studied in lipid production. Algal cells were cultured in autotrophic conditions under different concentrations of lovastatin. The results showed that the prevention of mevanolate pathway leading to decrease of cell growth, chlorophyll and beta-carotene concentrations, and as well as an increase in lipid production in D. salina, whereas in D. bardawil caused a decrease in the concentration of chlorophyll and beta-carotene, as well as an increase in lipid production with no affect on cell growth. The results showed, twice amount of chlorophyll and beta-carotene content in D. bardawil compared to D. salina and double growth of D. salina than D. bardawil. An increase of 1.5 times the lipid production in the shortest time (one week) in D. bardawil compared to D. salina showed that D. bardawil algae is more appropriate species for the production of lipid by inhibiting the mevanolate pathway. The results suggest that the mevanolate pathway is the required route for D. salina and D. bardawil algae, and preventing it, leading to change the pathway of acetyl coenzyme A from beta-carotene synthesis to increase of lipid production in the cell. LA eng UL http://jispp.iut.ac.ir/article-1-1037-en.html M3 ER -