Showing 2 results for Phenol.
Dr. Rouhollah Karimi, Dr. Behrooz Mohammadparast, Mrs Raziyeh Minazadeh,
Volume 8, Issue 32 (10-2019)
Abstract
Supplemental nutrition through affecting both enzymatic and non-enzymatic protective systems can modulate the oxidative stress induced by salinity. In this study the interaction effect of foliar application potassium (0 and 1.5% potassium sulfate; PS) and salinity (0, 25, 50 and 100 mM sodium chloride) on membrane degradation indices, phytochemical responses and antioxidant enzymes activities and also leaves nutrient elements of Bidaneh-Sefid grapevine was investigated as a factorial experiment based on a completely randomized design. By increasing salinity, hydrogen peroxide and malondialdehyde were increased in vines leaves under salinity stress. However the application of PS at 1.5% decreased the concentration of these indices 20 and 26 percentage respectively. As the salinity increased, the phenolic and flavonoid content of the leaves increased in the vines, however, foliar application of PS at 1.5% increased their content at the higher level in PS-treated vines. Moreover, in PS- treated vine at 1.5% soluble proteins increased dramatically even in high NaCl levels. Catalase and ascorbate peroxides activity increased until 50 Mm salinity stress, while with PS at 1.5% treated vine, the activities of these enzymes increased until 100 Mm NaCl. Potassium application also increased these enzymes activities at all levels of salinity. Also, the interaction of salinity and PS increased guaiacol peroxidase activity. Iron, zinc and leaf manganese concentrations increased due to the interaction of salinity and potassium. Totally, PS through the accumulation of soluble proteins, flavonoids and phenol, as well as increasing the activity of antioxidant enzymes finally reduced the oxidative stress induced by salinity in the vines leaves.
Amir Moradi Sarabshelli, Sayyed Kamal Kazemitabar, Morteza Ebrahimi, Ghafar Kiani, Morteza Khan Ahmadi,
Volume 9, Issue 36 (4-2020)
Abstract
St. John’s wort is a traditional herbal medicine used to treat depression and wound healing. Natural resource dependency and traditional cultivation methods often require months and years of time to reach medicinal plants. Establishing a hairy root suspension culture system can accelerate proliferation on a large scale, improve breeding and protect the species, but optimization and production on a large scale require an increase in the biomass and secondary metabolites of the root. In this study, the effect of different concentrations of myo-inositol (50, 75, 100, 125, 150, 175, 200, 225, 250, and 275 mg / l) were evaluated on biomass, phenol, flavonoid, DPPH activity and secondary metabolites in St. John's wort adventitious root in Agricultural Biotechnology Research Institute-Central Region of Iran. The results showed that 125 and 150 mg L-1 myo-inositol concentration had the highest dry weight, growth rate and growth ratio. The concentrations of 100 and 250 mg L-1 myo-inositol have been showed the highest and lowest amount of hypericin and pseudohypercin, respectively. From the viewpoint of production of hyperforin, the treatment with 100 mg L-1 myo-inositol was highest and the concentration of 125 mg L-1 had the least effect on the level of hyperforin in St. John's wort adventitious root culture. There was a significant difference between treatments in terms of phenol and flavonoid content, so the concentration of 75 and 225 mg L-1 and 75 mg L-1 myo-inositol had the highest amount of phenol and flavonoids. The DPPH activity changes were different between treatments, and the 50 mg L-1 showed the lowest amount of DPPH activity. However, the DPPH activity was highest in treatment with 75 mg L-1 myo-inositol concentration. Since the potential for stimulating secondary metabolites varies among cultures, elicitors, conditions for applying different treatments and other parameters, further research is needed to optimize better and more efficient guidelines.
