Showing 36 results for Malondialdehyde
Roozbeh Farhoudi,
Volume 1, Issue 1 (12-2012)
Abstract
Abstract:
In this experiment, the response of canola cultivars to salnity stress in germination stage was evaluated using a factorial experiment with four replications in completely randomized design. Six canola cultivars (Fornex, Alice, Orient, Consoul, Okamer and Okapi) were subjected to four salinity levels (0, 40, 80 and 120 mMol NaCl) under room, greenhouse, field? Salinity reduced the percentage of seed germination, seedling vigor index, α-amylase activity and seedling fresh weight, but it increased the mean germination time, malondialdehyde concentration, and cell membrane leakage. At the highest salinity level, Orient and Fornex cultivars had the highest germination percentage (84% and 80%), α-amylase activity (9.3 nmol seed-1 min-2 and 9.4 nmol seed-1 min-2) and seedling fresh weight (0.95 mg and 0.70 gr) compared to other cultivars. The electrical leakage of the seedling was lowest in the latter cultivars. Results showed a positive correlation of canola seedling weight with α amylase activity but negative correlations with the malondialdehyde concentration and cell membrane leakage. Salinity stress increased cell membrane damage and decreased α amylase activity, canola seed germination and seedling growth. Results indicated that under salt stress condition, Okamer and Okapi was sensitive canola cultivars compare with other cultivars.
Roozbrh Farhoudi, Adel Madhaj,
Volume 3, Issue 7 (9-2014)
Abstract
In order to evaluate the allelopatic potential of sunflower alcoholic extract against winter wild oat (Avena ludoviciana) at germiantion and vegetative growth stage two expriments were coducted in Islamic Azad University, Shoushtar branch at 2012. Both expriments were laid out according to Completely Randomized Design with five replications and treatments were various concentration of sunflower extract (0, 5,10,15 and20 gr L-1). Results indicated sunflower alcoholic extract application exhibited gradual rise inhibitory effect on seed germination, seedling weight, antioxidant enzymes activity and -amylase enzyme activity but elevated the mean germination time and malondialdehyde concentration in winter wild oat seedlings. The lowest -amylase enzyme activity was noted at higher levels of extract application (1.1 nmol seed -1 min-2). Likewise, seedling weight, antioxidant enzyme activities and sucrose synthetesis enzymes activity were declined with sunflower extract application but malondialdehyde concentration increased. Menimum sucrose synthesis activity (1.36 nmol prot -1 min-2 ) and highest malondialdehyde concentration (0.96 nmol grfw-1) was noted at 20 grL-1 concentration of sunflower alcoholic extract application. In conclusion, sunflower alcoholic extract decreased seed germination, seedling growth, -amylase enzyme and sucrose synthetesis enzymes activity of winter wild oat.
Naser Karimi, Zahra Souri,
Volume 4, Issue 11 (6-2015)
Abstract
Naser Karimi* and Zahra Souri2
Department of Biology, Razi University, Kermanshah, Iran
(Received: 13 December 2013, Accepted: 11 May 2014)
Abstract:
Arsenic is considered as one of the most important environmental contaminant compounds. Arsenic is known to induce oxidative stress in plants by generating various reactive oxygen species and thus increased lipid peroxidation. Phosphorus has some physic-chemical similarities with arsenic which affects its metabolism in plant cell. Malondialdehyde (MDA) is a measure of lipid peroxidation in membrane damage index. In this study, the interaction of arsenic and phosphorus on chlorophyll content and MDA accumulation of I. cappadocica, as a newly-discovered As hyperaccumulator, were investigated in hydroponic experiment. Plants were grown in a medium, embedded with combinations of 50, 200, 800 & 1200 μmol l−1 arsenic and 5, 50, 200, 800 & 1600 μmol l−1 phosphorus, respectively. The chlorophyll and MDA cnotent, hydrogen peroxide (H2O2) concentration, arsenic and phosphorus concentration and boimass of harvestable parts were determined. The highest concentration of arsenic was obtained in plants treated with 1200 μmol l−1 as and 5 μmol l−1 phosphorus. Increased levels of phosphorus led to reduction of arsenic concentration, which confirmed there was an interaction between these two elements in plant. Along with a marked increase in arsenic concentration in the media, a built up in (MDA) and (H2O2) were observed. The insignificant reduction in biomass and chlorophyll content, illustrated the high resistance of this herb to arsenic.
Rashid Jamei, Soroush Kargar Khorrami,
Volume 4, Issue 11 (6-2015)
Abstract
In this research the effect of four concentrations of nickel and copper (25, 50, 100, and 200 µM) on root and shoot length, fresh and dry weight, leaf surface, and photosynthetic pigments (Chlorophyll a, b and carotenoids), sugar, malondialdehyde, hydrogen peroxide content and antioxidant enzyme activity (CAT and APX) in Okra plants were evaluated. The results showed that in those plants which were treated with all concentrations of copper and nickel, the content of photosynthetic pigments and leaf surface were reduced significantly in comparison with control. In this investigation it was found that shoot and root length, dry weight and fresh weight of shoot and root which were treated with concentrations 50, 100, and 200 µM of cupper and 100 and 200 µM of nickel were reduced significantly when compared with the control. Content of soluble sugars in shoot and root plants which were treated with all concentrations of nickel and concentrations 50, 100, and 200 µM of cupper increased significantly in comparison with the control. Also, content of malondialdehyde, hydrogen peroxide content and antioxidant enzyme activity (CAT and APX) increased significantly in shoot and root plants which were treated with concentrations 50, 100, and 200 µM of copper and nickel when compared with control.
Masume Ganji, Esfandiar Farahmandfar, Maryam Shahbazi, Mehdi Zahravi,
Volume 5, Issue 15 (4-2016)
Abstract
Drought is one of the most significant a biotic stresses that affecting plant’s growth and development wild barley, H.spontaneum, the progenitor of cultivated barley has a high potential in terms of tolerance to environmental stresses. This research was performed on nine genotypes of Hordeum spontaneum, to study drought tolerance in a split plot experiment based on Randomized Complete Block Design with three replications under field shelter in 2011-2012. The main plot included three levels of water stress as normal irrigation, water withholding at flowering stage and no irrigation and sub-plots comprised the studied genotypes. The results indicated that the effect of drought stress on plant height, grain yield, biomass, relative water content, osmotic adjustment, stomatal conductivity, chlorophyll content, catalase, ascorbate peroxidase and peroxidase, protein, malondialdehyde, and proline was significant (P<0.01). The results of principal component analysis on the whole evaluated traits in drought stress condition located the studied genotypes in four groups. The first and second group comprised genotypes 6, 4 and 5. These genotypes had significantly higher grain yield, relative water content, chlorophyll content, enzymatic activity of peroxidase and ascorbate peroxidase and lower content of malondialdehyde, leaf temperature and osmotic potential (more negative) and therefore, were identified as tolerant genotypes.
Roozbeh Farhoudi, Ali Soheli Far, Adel Modhej,
Volume 5, Issue 17 (9-2016)
Abstract
In order to evaluate the allelopatic potential of artichoke (Cynara cardunculus) on germination, growth, lipid peroxidation and some hormones content of Cyperus rotundus rhizome were assessed in Islamic Azad University, Shoushtar Branch (Iran). The experiments were carried out under completely randomized design with 6 treatments (0, 20%, 40%, 60%, 80% and 100% C. cardunculus aqueous extract as irrigation water) in 5 replications. Remarkable decreases were observed in seedling fresh weight, shoot height, α-amylase activity, Indole acetic acidand Gibberellins content of C. rotundus rhizome in line with the increase in C. cardunculus extract percentage. Lowest α-amylase activity (1.25 nmol gr rhizome min-1), Indole acetic acid (50 µg gr-1 ) and Gibberellins (95 µg gr-1) in C. rotundus rhizome obtained from 100% C. cardunculus aqueous extract. Malondialdehyde and Abscisic Acid content reached its top concentration in C. rotundus rhizome (0.92µmo-1 gr FW and 172 µg gr-1) treated with 100% C. cardunculus aqueous extract as irrigation water. This study supported the assumption argued that C. cardunculus extract inhibited the C. rotundus rhizome growth through increasing lipid peroxidation and decreased Indole acetic acid and Gibberellins content.
Mohammadreza Moraditelavat, Khalil Alamisaeed, Aziz Karmollachaab, Hadis Hasanvand,
Volume 6, Issue 19 (3-2018)
Abstract
A factorial experiment in hydroponic conditions was conducted to evaluate the activity of antioxidant enzymes, membrane stability and Na concentration in seedling of some rice cultivars, in 2014 summer in Ramin Agriculture and Natural Resources University of Khuzestan. Experimental factors were included salinity levels of NaCl (0, 4 and 8 ds.m-1) and rice cultivars (Amol3, Daniel, Shafagh, Fajr and Red Anboori). Some attributes including, hydrogen peroxide, malondialdehyde, peroxidase, electrolyte leakage, Na concentration and shoot dry weight were measured. The result showed that salinity levels significantly decreased shoot dry weight and increased antioxidant enzymes, membrane peroxidation, electrolyte leakage, and Na concentration. Rice cultivars responded differently to salinity levels. Red Anboori and Amol3 Cultivars had the highest antioxidants and the lowest lipid peroxidation. These two cultivars in control condition had lowest shoot dry weight (5.15 and 5.51 g.plant-1). However, these cultivars had no significant difference with other cultivars in 8 dS.m-1 salinity condition. On the other, Danyal and Fajr cultivars were obtained greatest reduction in shoot dry weight under severe stress (51.9 and 49.4 percent respectively). So Red Anbory and Amol 3 cultivars by increasing enzymatic defense system and reduce damage to cell membranes as tolerant cultivars at the seedling stage compared to another cultivars will be introduced.
Dr. Mohsen Movahhedi Dehnavi, Mrs Marzieh Misagh, Dr. Alireza Yadavi, Mrs Mitra Merajipoor,
Volume 6, Issue 20 (9-2017)
Abstract
Micronutriens deficiency is prevalent in case of drought stress conditions, so complementary boron and zinc nutrition are important for crops to overcome drought stress. This study evaluated the effect of zinc and boron foliar application on physiological traits of sesame under different irrigation levels. The experiment was conducted as a split plot in a randomized complete block design with three replications at Fars Research Center for Agriculture and Natural Resources in 2013. The main factor included three irrigation levels (irrigation after 75, 110 and 145 mm evaporation from class A evaporation pan) and sub-factor included foliar spraying water, zinc sulphate, boric acid and application of the mixture of zinc sulphate with boric acid. Results showed that proline content of leaf significantly increased by foliar application of boric acid in 75 mm evaporation treatment and by boric acid and mixture of zinc sulphate and boric acid in 110 and 145 mm evaporation treatments. Maximum Glycinebetaine was acquired by foliar zinc sulphate application in all three irrigation levels. Foliar application of zinc sulphate and boric acid helped to obtain the highest leaf relative water content compared to control. Maximum seed zinc content in 75 mm evaporation treatment was obtained from zinc sulphate and the mixture of zinc sulphate with boric acid application. Generally, results showed that foliar application of zinc sulphate and boric acid improved physiological traits of sesame, especially under drought stress so the plant can tolerate the impact of drought stress better.
Siavash Safari, Javad Erfani-Moghadam, Mohammad Javad Zarea,
Volume 6, Issue 21 (11-2017)
Abstract
Salinity of water irrigation and soil are of the major factors affecting plant growth and productivity worldwide. In this research the effects of salicylic acid on some morpho-physiological and biochemical characteristics of walnut (Juglans regia) seedlings under salinity stress condition were studied. The experiment was a two-factor factorial experiment arranged in completely randomized design with three replications. Two factors of the experiment were (or included) salicylic acid (0, 1, 2 and 3 mM) and NaCl (0, 50, 100 and 150 mM) that their affecting was evaluated when walnut seedlings were 60- day old. Analysis of variance has illustrated that a significant difference exists among treatments for most of studied traits. Increasing salinity levels resulted in reducing leaf number, leaf area, branch number and shoot length in walnut seedlings. While induced salinity significantly decreased chlorophyll pigments and leaf relative water content (RWC) but enhanced electrolyte leakage, malondialdehyde (MDA) and proline concentration. Pre-treatment seedlings with 1 mM salicylic acid provided protection against salinity stress in walnut seedling. The interaction between salinity and salicylic acid was significant for some measured characters such as carotenoids, leaf relative water content, electrolyte leakage, malondialdehyde and proline. Overral, the results from this experiment showed that applying salicylic acid at the concentration level of 1 mM, caused to reduction of tissue destruction during salinity stress and are suitable treatment for maintenance walnut seedling.
Zohreh Jabbarzadeh, Parvin Talebi,
Volume 7, Issue 23 (5-2018)
Abstract
This experiment was conducted to investigate the positive effects of foliar and soil application of humic acid on chlorophyll content, flower longevity, antioxidant enzymes activity, proline, malondialdehyde and protein content in a completely randomized design with two factors: humic acid at 4 concentrations (0, 500, 1000 and 2000 ppm) and 2 application method of humic acid (foliar spray and drench) with 3 replications and 2 observations in Rosa chinensis ‘Baby Masqurade’. The results showed that both soil and foliar applications of humic acid caused to increase chlorophyll and protein content, antioxidant enzymes activities and flower longevity respectively about to 2, 5, 3 and 1.75 fold compared to control. MDA levels were increased in soil treatments with increasing concentrations of humic acid but inversely were decreased in foliar treatments. This process is repeated in the activity of APX enzyme. Catalase activity was increased in low concentrations of humic acid but with increasing concentration, its activity was declined. The total protein was increased in all of the humic acid concentrations (except in 500mg/l HA as soil application) compared to control. The results of this experiment showed that foliar and soil application of humic acid with increasing the activity of antioxidant enzymes and chlorophyll and protein content of plant cause to increase the longevity of flowers.
Mrs Samaneh Asadi-Sanam, Mrs Seyede Mohadeseh Mohammadi, Mr Valiollah Rameeh, Mr Mahyar Gerami, Mr Majid Khosh Rooz,
Volume 7, Issue 23 (5-2018)
Abstract
In order to evaluate the effect of sodium nitroprusside (SNP) as an effective antioxidant with advantageous impact in reducing oxidative stress in purple coneflower (Echinacea purpurea) seedlings under saline condition, a factorial experiment with arrange of completely randomized design with four replications was conducted at Sana Institute of Higher Education in 2015. In this study, the effect of the SNP as doner of nitric oxide (NO) at 4 levels ( 0, 0.1, 0.2 and 0.4 mM) on some biochemical characteristics of purple coneflower under 3 levels of salinity stress (0, 75 and 150 mM of NaCl solution) were evaluated. The results showed that the interaction between SNP and salinity on fresh and dry weight of root and leaf, carotenoid and malondialdehyde (MDA) content, enzyme activities of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) were significant. With increasing of salinity concentration, root fresh and dry weights were decreased, while the traits were increased by 0.2 mM of SNP under salinity. The highest of leaf fresh and dry weight were observed in the control treatment. The chlorophyll a, b and total chlorophyll contents were decreased with increasing of salinity, while the highest amount of chlorophyll were measured 0.4 mM SNP under normal conditions. The highest level of total phenol was observed in the highest level of salinity and a higher level of MDA was also observed in control plants under salinity stress. Protein content was also decreased 5.4 % under salinity stress. The results also showed that 0.2 mM of SNP pretreatment was increased activities of antioxidant enzymes SOD, POD and APX in plants under the stress
Jafar Amiri,
Volume 7, Issue 24 (8-2018)
Abstract
Salt stress is one the major environmental stresses limiting quantitative and qualitative aspects of plant growth. One of the methods to overcoming the adverse effects of salinity is application of growth regulators. Salicylic acid is a plant growth regulator its positive effects in alleviating the adverse effects of stresses on plants have been reported. In order to study the effect of salicylic acid (SA) on some morphological, physiological and biochemical characteristics of grapevine, (Vitis vinifera cv. Thompson Seedless), a pot factorial experiment was conducted as randomized complete design, under salinity stress condition. Well-rooted grape cuttings were exposed to five levels of salinity 0 (control), 25, 50, 75 and 100 mM NaCl and four levels of SA, 0 (control), 100, 200 and 300 mg/l. The results indicated that with the increase in salinity levels, the morphological traits, and leaves relative water contents were reduced, leaves cells membrane leakage and the amount of tissues malondialdehyde increased. In salinity level of 100 mM, the length of stem and root was reduced 67.18 and 59 percent in compared to control. In salinity level of 100 mM, application of 300 mg/l salicylic acid, increased proline and soluble solids 4.11and 7.5 times respectively compared to control. It also increased guaiacol peroxidase, ascorbate peroxidase and catalase 3.15, 4.1 and 3.6 times respectively compared to control. Overall, the application of SA (200-300 mg/l) caused increase in growth efficiency, antioxidant enzymes activities and the amount of compatible osmolytes but reduced the ionic leakage.
Mrs. Sanaz Sarrafi, Mr. Arman Azari, Mr. Rouhollah Saberi Riseh, Mr. Ali Akbar Mohammadi Mirik,
Volume 7, Issue 24 (8-2018)
Abstract
To study the effect of bacterial siderophore on the second cropping of maize (SC645) under drought stress conditions, a factorial experiment was arranged in a randomized complete block design with three replications. Two irrigation treatments (75 (I1: unstressed control) and 120 mm evaporation from Class-A pan (I2: delayed irrigation)) and two siderophore application treatments (S1: with and S2: without application) were considered as experimental factors. The foliar application of siderophore (2000 mg l-1) were applied two times at 4-6 and 12-14 leaf growth stages. In addition, 20 Kg siderophore ha-1 was applied with irrigation at jointing stage. The results showed leaf pigments, leaf relative water content (RWC), membrane stability, leaf area index(LAI) and specific leaf area (SLA) increased at I1 compared to I2, while sucrose (saccharose), lipids peroxidation (MDA), guaiacol peroxidase and polyphenol oxidase activities decreased. S1 compared to S2, showed increased leaf pigments, the ability of osmotic adjustment, antioxidant enzymes activities, RWC, membrane stability, LAI and SLA and decreased MDA. The interaction effect showed that siderophore application increase chlorophylls (a, b and total), proline and soluble sugars of leaf in each irrigation levels. The highest forage fresh yield (8697 gm-2) belonged to I1. S1 increased forage fresh yield (1644 gm-2) compared to S2. In general, bacterial siderophore can improve the morphological, physiological and biochemical capabilities of maize and is effective in mitigating the effects of drought and in increasing photosynthetic capacity.
Nasrin Razmi, Ali Ebadi, Jahanfar Daneshian, Soodabe Jahanbakhsh,
Volume 7, Issue 26 (11-2018)
Abstract
Abstract:
In order to evaluate the effect of drought stress and salicylic acid on mineral content and some physiological traits of soybean genotypes (Glycine max L.) a greenhouse experiment was conducted as factorial based on completely randomized design with three replications in Mohaghegh Ardabil University in 2014. The first factor consisted of three levels of water deficit (85%, 65% and 45% field capacity), the second factor consisted of three concentration of salicylic acid (0 or distilled water, 0.4 and 0.8 mM) and the third included soybean genotypes (Williams, L17 and D42X19). The results showed that water deficit increased the amount of sodium, sodium to potassium ratio, electrolyte leakage, malondialdehyde and glycine betaine and decreased leaf relative water content and leaf area per plant. Soybean genotypes responded differently to water deficit. Williams and L17 had the highest and lowest concentrations of calcium, potassium, relative water content and leaf area per plant, respectively. Salicylic acid decreases the amount of lipid peroxidation, electrolyte leakage and increased concentrations of glycine betaine and leaf area per plant. Interaction of salicylic acid and genotype on traits was significant. Application of 0.4 mM salicylic acid exhibited afavorable effects on the accumulation of calcium, potassium, phosphorus and increasing the relative water content and maintaining the integrity of cell membranes and ameliorated the inhibitory effects of water deficit in soybean genotypes especially in Williams. Foliar application with 0.4 mM salicylic acid reduced adverse impact of water deficit stress in Williams cultivar.
Phd Masoud Khazaei, Phd Mohammad Galavi, Phd Mahdi Dahmardeh, Phd Seyd Mohsen Moosavi-Nik, Phd Gholamreza Zamani, Phd Nafiseh Mahdi-Nejad, Phd Zohreh Alizadeh,
Volume 7, Issue 27 (2-2019)
Abstract
Water shortage is one of the most important limiting factors for crop production, that affected growth and yield by changing physiological conditions. This study was carried out to investigate effect of oxidative stress on antioxidant defense mechanism in Research greenhouses of Birjand University in 2014. A factorial arrangement of treatments based on randomized complete block design (Due to the lack of uniformity of light) with four replications was used .The studied factors were drought stress with three levels including 100, 75 and 50 percent of plant water requirement (non-stress, moderate and severe stress respectively) and millet genotype with three levels (including Bastan, promising lines KFM5 and KFM20).. Malondialdehyde, electrolyte leakage and membrane stability was measured in 30 and 45 days after stress and yield in maturity. Antioxidant activity (including SOD, CAT and APX) was measured in 30 days after stress. The results showed that antioxidant activity increased as intensity of drought stress increased in all three genotypes. Bastan had the highest antioxidant activity under sever stress that showed it had best antioxidant defense system and better scavenge reactive oxygen species than another genotypes. The malondialdehyde content (MDA) was low in Bastan compared to other genotypes and was less affected by increasing drought stress that showed this genotype was less affected by oxidative stress. Seed yield decreased as intensity of drought stress increased in all three genotypes and had negative correlation with antioxidant activity.
Mrs Batool Zarei, Mr Arash Fazeli, Mrs Zahra Tahmasebi,
Volume 8, Issue 29 (5-2019)
Abstract
Black cumin (Nigella sativa) is an annual flowering plant in the family Ranunculaceae that beside medicinal value was used as food and cosmetics products. Salicylic acid is a growth regulator with phenolic nature has main role in regulation of many physiological processes and resistance to environmental stress. In order to study effect of salt stress and salicylic acid on some growth parameters including chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, proline, malondialdehyde (MDA), other aldehydes, gayvkl peroxidase and lipoxygenase in medicinal plant Nigella (Nigella sativa L.), an experiment was conducted in factorial design based on complementary randomized design in 2016 at greenhouse of Ilam university. Treatments were include salinity at three level (Zero, 25 and 75 mM) and salicylic acid at three level (Zero, 0.75 and 1.5 mM) respectively. The results showed that salt stress decreased chlorophyll a, chlorophyll b, total chlorophyll, carotenoids and a significant increase in proline, malondialdehyde (MDA) and other aldehydes. Black cumin is sensitive to salinity stress, so by applying salicylic acid antioxidant capacity of the plants were increased against stress conditions.
Dr Halimeh Hassanpour, Dr Vahid Niknam,
Volume 8, Issue 30 (5-2019)
Abstract
Mechanical vibration is one of abiotic stresses which could affect on growth and development of plant cells. In this research, effect of sinusoidal vibration were studied on some physiological parameters, protein, total phenols and free radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) in Anthemis gilanica seedlings. Sinusoidal vibrations were applied at 0, 10, 15, 50 and 100 Hz on 10 days seedlings, and were analyzed for physiological and biochemical responses after 30 days. Results showed different responses of growth, antioxidant activity and phenolic content under different frequencies of vibration. Sinusoidal vibrations at frequencies of 15 and 50 Hz resulted in a significant increase in growth parameters, relative water content, chlorophyll a and chlorophyll b and protein content compared to control. Malondialdehyde (MDA) and H2O2 levels decreased significantly at frequencies higher than 10 Hz, and 15 Hz treatment resulted in a 54.55% and 24.5% reduction of hydrogen peroxide and malondialdehyde content as compared to control. Sinusoidal vibrations also increased in free radical scavenging activity by reducing the IC50 value, and the highest decrease was observed at 50 Hz frequency. It seems that increase of antioxidant capacity under proper frequency of vibration can induce A. gilanica growth.
Dr Mohsen Pourgholam, Dr Mohammad Nasri, Dr Farshad Ghoshchi, Dr Hamid Reza Tohidimoghadam, Dr Hamid Reza Larijani,
Volume 8, Issue 30 (5-2019)
Abstract
In order to investigate the effect of drought stress by application of hormone and nanoparticulate spraying on corn biochemical traits, a split factorial was used in a randomized complete block design with four replications at the Agricultural Research Faculty of Islamic Azad University, Varamin. Drought stress in two levels (drought stress and drought stress) and three growth regulators (application of gibberellic acid (200 ppm), application of cytokine (200 ppm) and lack of application of regulator and growth hormone) and three levels of nano Particles (application of silver nanoparticles (0.02%), application of zinc nanoparticles (0.02%) and application of nanoparticles were considered. The results showed that in all drought stress conditions, the traits under study were affected so that the levels of malondialdehyde, proline, superoxide dismutase, catalase increased and leaf protein, alpha-amylase and soluble sugar decreased. The application of gibberellin and cytokinin as well as application of silver and zinc nanoparticles reduced the effects of drought stress. In drought stress conditions, growth regulator application and nanoparticles resulted in increased proline levels. The use of gibberellin hormone with the application of silver and zinc nanoparticles reduced the amount of protein in the leaf. According to the results, the application of growth regulators and nanoparticles can help improve plant performance under drought stress conditions.
Mr Nasrollah Soori, Dr Davood Bakhshi, Dr Abdolhossein Rezaei Nejad, دکتر Mohammad Faizian,
Volume 8, Issue 32 (10-2019)
Abstract
Water and soil salinity are one of the most important restrictions on the growth and production of fruit trees. Control of this phenomenon can be one of the factors to managing the production and guarantee the production sustainability and optimal use of land and water. Therefore, in order to investigate the effect of salinity stress on the physiological and biochemical properties of some Iranian commercial pomegranate cultivars and identification of salinity tolerant cultivars, a greenhouse research conducted at the Faculty of Agriculture of Lorestan University in 2015. The Factorial experiment based on randomized complete block design with five levels of salinity (0, 25, 50, 75 and 100 mM NaCl) and six pomegranate cultivars include Malas Dane Ghermez Esfahan, Malas Yazdi, Shirin Shahvar, Meykhosh Yazd, Malas Saveh and Malas Yousofkhani and with three replications. After three months of applying salt stress, some physiological and biochemical properties of the plants were examined and measured. The results showed that in control treatment, compared to 100 mM NaCl treatment with increasing salinity, chlorophyll a (42%), chlorophyll b (40%), total chlorophyll (42%) and total carotenoids (65%) decreased significantly and Electrolyte leakage rate (27%), malondialdehyde (94%), and peroxidase (506%), catalase (478%), and ascorbate peroxidase (366%) enzymes increased. In addition, comparison of data meanings showed that there was a significant difference between cultivars in measured factors. The results showed that, in salt stress conditions, Malas Yousofkhani cultivar was superior to salinity tolerance in most traits, and this genotype was more tolerant to salinity than other evaluated cultivars.
Moazameh Salimi, Shahab Madah Hosseini, Arman Azari, Ali Akbar Mohamadi Mirik,
Volume 8, Issue 33 (12-2019)
Abstract
In order to study the effects of cold stress on some physiological characteristics of three pre-selected of linseeds, a factorial experiment based on completely randomized design with two factors and three replications was carried out in growth chamber of Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, IRAN. The first factor was cold treatment at three levels (25, 0 and -2.5 OC) and the second factor was genotype including three accessions named MAR778b, CYP706 and IND1257 selected based on preliminary test as tolerant, semi tolerant and sensitive to cold, respectively. The results showed that temperature decrease from 25 to 0OC did not induce any significant change in any traits. On the contrary, -2.5 OC treatment significantly increased content of total soluble sugars, proline and malondialdehyde IND1257 (susceptible) much more, and chlorophyll content, much less than two other genotypes. Also, total leaf carotenoids content of tolerant genotype (MAR778b) at -2.5 OC was significantly lower companed to the control (25 OC), though those of two other genotypes were not different. Finally, poly phenol oxidase activity decreased by 0 and -2.5 OC in all three genotypes but phenyl alanine amonialyase increased at -2.5 OC compared to 0 and 25 OC only in tolerant cultivar (MAR778b). Correlation analysis revealed a significant reverse association of PAL activity with stress susceptibility index.
