Amir Moradi Sarabshelli, Sayyed Kamal Kazemitabar, Morteza Ebrahimi, Ghafar Kiani, Morteza Khan Ahmadi,
Volume 9, Issue 36 (4-2020)
Abstract
St. John’s wort is a traditional herbal medicine used to treat depression and wound healing. Natural resource dependency and traditional cultivation methods often require months and years of time to reach medicinal plants. Establishing a hairy root suspension culture system can accelerate proliferation on a large scale, improve breeding and protect the species, but optimization and production on a large scale require an increase in the biomass and secondary metabolites of the root. In this study, the effect of different concentrations of myo-inositol (50, 75, 100, 125, 150, 175, 200, 225, 250, and 275 mg / l) were evaluated on biomass, phenol, flavonoid, DPPH activity and secondary metabolites in St. John's wort adventitious root in Agricultural Biotechnology Research Institute-Central Region of Iran. The results showed that 125 and 150 mg L-1 myo-inositol concentration had the highest dry weight, growth rate and growth ratio. The concentrations of 100 and 250 mg L-1 myo-inositol have been showed the highest and lowest amount of hypericin and pseudohypercin, respectively. From the viewpoint of production of hyperforin, the treatment with 100 mg L-1 myo-inositol was highest and the concentration of 125 mg L-1 had the least effect on the level of hyperforin in St. John's wort adventitious root culture. There was a significant difference between treatments in terms of phenol and flavonoid content, so the concentration of 75 and 225 mg L-1 and 75 mg L-1 myo-inositol had the highest amount of phenol and flavonoids. The DPPH activity changes were different between treatments, and the 50 mg L-1 showed the lowest amount of DPPH activity. However, the DPPH activity was highest in treatment with 75 mg L-1 myo-inositol concentration. Since the potential for stimulating secondary metabolites varies among cultures, elicitors, conditions for applying different treatments and other parameters, further research is needed to optimize better and more efficient guidelines.
Farahnaz Tavakoli, Mohammad Rafieiolhossaini, Rudabeh Ravash, Morteza Ebrahimi,
Volume 10, Issue 44 (7-2021)
Abstract
The use of Abiotic elicitors is very important in biosynthetic pathways that lead to the formation and regulation of secondary metabolites. The aim of this study was to investigate the effect of Low temperature elicitors on the amount of hypericin, flavonoids, phenol, antioxidant capacity and dry weight of ).Seeds of Topaz cultivar, Pharmasat Germany, with 99% purity and 85% potency, were prepared for the ability to produce secondary metabolites. Seeds were grown in MS medium in sterile containers and stored in a growth chamber at 25±1 ° C with light rotation for 8 hours in the dark and 16 hours of light with fluorescent light. This study was performed as a factorial study in a completely randomized design with three replications and the effect of temperatures of 4, 8, 16 and 25 ° C for 24 hours, 72 hours and one week. The highest amount of hypericin at 4 ° C for one week and 72 hours with 0.59 and 0.55 μg / g dry matter, respectively, and the highest antioxidant capacity at 4 ° C for one week at 0.87% was observed. With increasing temperature to 16 degrees (for 24 hours) the lowest amount of hypericin and antioxidant capacity was observed. Applying a temperature of 4 degrees for a week increased the amount of flavonoids. The highest amount of phenol was obtained in the samples treated at 4 ° C for one week and 72 hours with the amount of 5.65 and 5.03 mg / g dry matter, respectively. Applying 4 ° C for one week had the greatest effect on the dry weight of the plant. Due to the increase in secondary metabolites of hypericin, phenol, flavonoids and antioxidant capacity of H. perforatum after application of 4 ° C treatment (for one week and 72 hours), the use of this elicitor to increase secondary metabolites of H. perforatum Recommended.
