Mohsen Sanikhani, Arefeh Rastgoo, Azizollah Kheiry, Mitra Aelaei,
Volume 9, Issue 36 (4-2020)
Abstract
To study the effect of foliar application of proline and salicylic acid on steviol glycosides and phenolic of Stevia, an experiment was conducted based on a completely randomized block design in a factorial arrangement with three replications in the University of Zanjan. Foliar application of proline in three levels (0, 1, 2 mM) as well as salicylic acid (0, 0.3, 0.6 mM) were applied after establishment of transplants in the field three time with 10 days intervals. Results showed that salicylic acid and proline applications significantly influenced on steviol glycosides, phenol and flavonoid. The treatment with salicylic acid had positive effects on all steviol glycosides, phenol and flavonoid, but proline only enhanced contents of glycosides stevioside, rebaudioside A, phenol and flavonoid. The highest average steviol glycosides stevioside (60.32 mg/g dw), rebaudioside A (24.14 mg/g dw) and total phenol (19.94 mg/g dw) was recorded in 1 mM proline in combination with 0.6 mM salicylic acid. The highest average flavonoid (10.02 mg/g dw), in 0.6 mM salicylic acid, and lowest average steviol glycosides and phenolic contents observed in control. Collectively, the results showed that application of proline and salicylic acid significantly improved production of active substances in Stevia rebaudiana.
Amir Moradi Sarabshelli, Sayyed Kamal Kazemitabar, Morteza Ebrahimi, Ghafar Kiani, Morteza Khan Ahmadi,
Volume 9, Issue 36 (4-2020)
Abstract
St. John’s wort is a traditional herbal medicine used to treat depression and wound healing. Natural resource dependency and traditional cultivation methods often require months and years of time to reach medicinal plants. Establishing a hairy root suspension culture system can accelerate proliferation on a large scale, improve breeding and protect the species, but optimization and production on a large scale require an increase in the biomass and secondary metabolites of the root. In this study, the effect of different concentrations of myo-inositol (50, 75, 100, 125, 150, 175, 200, 225, 250, and 275 mg / l) were evaluated on biomass, phenol, flavonoid, DPPH activity and secondary metabolites in St. John's wort adventitious root in Agricultural Biotechnology Research Institute-Central Region of Iran. The results showed that 125 and 150 mg L-1 myo-inositol concentration had the highest dry weight, growth rate and growth ratio. The concentrations of 100 and 250 mg L-1 myo-inositol have been showed the highest and lowest amount of hypericin and pseudohypercin, respectively. From the viewpoint of production of hyperforin, the treatment with 100 mg L-1 myo-inositol was highest and the concentration of 125 mg L-1 had the least effect on the level of hyperforin in St. John's wort adventitious root culture. There was a significant difference between treatments in terms of phenol and flavonoid content, so the concentration of 75 and 225 mg L-1 and 75 mg L-1 myo-inositol had the highest amount of phenol and flavonoids. The DPPH activity changes were different between treatments, and the 50 mg L-1 showed the lowest amount of DPPH activity. However, the DPPH activity was highest in treatment with 75 mg L-1 myo-inositol concentration. Since the potential for stimulating secondary metabolites varies among cultures, elicitors, conditions for applying different treatments and other parameters, further research is needed to optimize better and more efficient guidelines.
Hadiseh Sadeghi, Jafar Ahmadi, Farhad Habibzadeh, Sedigheh Fabriki Ourang,
Volume 13, Issue 60 (5-2024)
Abstract
Japanese barberry or ornamental barberry (Berberis thunbergii) belongs to the Berberidaceae family. This family have several properties, including antioxidant, antimicrobial and anticancer activities. The healing properties of barberry have been known and studied for thousands of years. In order to study the changes in the physiological-biochemical characteristics of Japanese barberry under the influence of abiotic elicitors, an experiment was conducted in the form of a completely randomized design in the greenhouse and laboratory of the Faculty of Agriculture and Natural Resources of Imam Khomeini International University in 2018-2019. The treatments included methyl jasmonate elicitors, salicylic acid, titanium dioxide and no elicitor (control) in four replications. In this research, the amount of leaf photosynthetic pigments and antioxidant enzymes of leaf and root tissues of Japanese barberry plant were extracted and measured. The results showed that the effect of the experimental treatments on the amount of photosynthetic leaf pigments and antioxidant enzymes in the two studied tissues was significant at the 1% probability level. Comparison of the means showed that salicylic acid treatment increased the amount of chlorophyll a and total leaf chlorophyll compared to the control by 20.9% and 21.3%, respectively. Salicylic acid and methyl jasmonate increased 29.9 and 19.1 percent of carotenoid content in leaves, respectively, compared to the control. The highest protein content of the root was produced with the use of salicylic acid, which increased by 38.6% compared to the control. The activity of catalase enzyme in the root increased by salicylic acid and titanium dioxide elicitors by 76.8 and 52.2%, respectively, compared to the control. In leaves, salicylic acid and methyl jasmonate elicitors increased catalase enzyme activity by 104.1% and 57.1%, respectively. From this research, it was concluded that salicylic acid had the greatest effect in increasing the amount of pigments and the activity of antioxidant enzymes of the Japanese barberry plant, which can be used for medicinal products.
