Farnush Jafari, Jaber Panahandeh, Alireza Motallebi Azar, Musa Torabi Giglou,
Volume 8, Issue 33 (12-2019)
Abstract
order to study some physiological traits and the changes of nutrient uptake in potato plantlets under in vitro conditions in response to osmotic and temperature stresses, this research was conducted as a factorial experiment in a block design. The first factor was osmotic stress induced by PEG - 6000 in four levels (0, -0.5, -1 and -1.5 MPa), and the second factor was temperature at three levels (15, 25 and 35 ° C). The third factor was potato plantlets consist three promising clones (143, 301 and 306) and two commercial cultivars (Marfona and Picasso), which were planted in MS medium. Eight weeks later, plantlet’s fresh and dry weights, number of developed leaves, the content of P, K, Ca and Na in above parts of plantlet were measured. The results showed that the simple effects of factors as well as their interactions at 1% level were significant. Mean comparison of treatments showed that the simultaneous effects of stresses reduced fresh and dry weights, leaf number as well as the uptake of P, K, Ca but Na uptake increased. According to the results, the highest fresh, dry weights were observed in non- heat stress conditions (25 ° C). On the other hand, increasing of temperature from 15 to 35 ° C, increased the absorption of P and Na, but the amount of K and Ca were greatest at 25 ° C (no temperature stress).
