Showing 6 results for Membrane Stability Index
, ,
Volume 6, Issue 21 (11-2017)
Abstract
One of ways to cope the drought stress is using tolerant plant and water preservative materials in the soil such as zeolite. Hemp is one of drug and oilseed plants.To evaluate the effect of zeolite in the modulation of deficit irrigation stress and improve the function and growth indices of Hemp, an experiment was conducted as split plot in a randomized completely block design with three replications at the research farm of Shahrekord university in 2014. The main factor including deficit irrigation stress in four levels (provide of 40, 60, 80 and 100% of water requirement) and the subplot was zeolite in three levels (0, 5 and 10 t/ha). The results showed that there was a difference in terms of trend of the Leaf Area Index (LAI), Leaf Area Duration (LAD), Biomass Duration (BMD), Crop Growth Rate (CGR) and Net Assimilation Rate (NAR) between levels of zeolite and irrigation regimes. Increasing the amount of irrigation water due to the presence of zeolite in the rhizosphere, increased the characteristics of LAI, LAD, BMD, CGR, and chlorophyll content (SPAD) on studied traits. Thus, according to zeolite role in setting the necessary conditions for the better growth of hemp, it seems that the use of 10 t.ha-1 zeolite is useful particularly in conditions of severe stress.
Mrs Hamideh Ghafari, Dr Mahmoud Reza Tadayon, Dr Jamshid Razmjoo,
Volume 7, Issue 26 (11-2018)
Abstract
In order to investigate the growth responses of sugar beet plant to drought stress and foliar application of proline at different growth stages, a field experiment was conducted as split plot arrangement in a randomized complete block design with three replications at the field Research Station of Shahrekord University during 2015. Main plot three level of irrigation treatments (100% (control), 75% and 50% of water requirement) and sub plot three level of proline applied (sprayed with water (control), 5 and 10 mM. The result showed that the drought stress reduced membrane stability index (MSI) and SPAD values and 10 mM proline foliar application causes plant resistance and enhanced MSI and SPAD values under drought stress as compared with the control (sprayed with water). The drought stress reduced leaf area index (LAI), crop growth rate (CGR), net assimilation rate (NAR) and shoot and root dry weights compared with full irrigation. The reduction rate and time in traits were higher on stress level than full irrigation and foliar application of 10 mM were low. There is highly correlation between LAI, CGR and shoot dry weight with root dry weight. So, proline function as compatible solute in osmotic adjustment for sugar beet with the protective role through physiological reactions under drought stress.
Mrs Hamideh Ghafari, Dr Mahmoudreza Tadayon,
Volume 8, Issue 29 (5-2019)
Abstract
Salinity is one of the major challenges to increasing crop productivity. Besides these, in order to investigate of proline and salicylic acid in the modulation of salt stress on soybean (Glycine max L.) , an experiment was conducted as split plot arrangement in a randomized completely design with four replication in 2016 in College of Agriculture, Shahrekord University. Main plot included three level irrigation by saline water with electrical conductivity 2 (control), 5 and 10 dS m-1 NaCl) and sub plot in four level of foliar applied (10 mM proline, in combination with 10 mM proline + 3 mM salicylic acid, 3 mM salicylic acid and sprayed with water (control)). The results showed that the 5 and 10 dS m-1 NaCl lead to increased and decreased of photosynthetic pigments compared with the control, respective. Proline and soluble sugars were increased under salt stress, while membrane stability index and relative water content decreased. Interaction between salt stress and foliar treatments showed that the highest increase in chl. b, carotenoid and proline were recorded in 5 dS m-1 NaCl and proline plus salicylic acid with increase of 93, 53 and 143% as compared to the control, respectively. Membrane stability index and relative water content were decreased 63 and 38% as compared to the control, respectively. So, foliar application of 10 mM proline with 3 mM salicylic acid on physiological parameters of soybean was the best treatment to mitigate the detrimental effects of salt stress. So, proline function as compatible solute in osmotic adjustment and salicylic acid with the protective role through physiological reactions under salt stress.
Afsaneh Nematpour, Hamid Reza Eshghizadeh,
Volume 8, Issue 33 (12-2019)
Abstract
In order to investigate the effects of drought stress, nitrogen chemical nutrition and sowing date on agrophysiological characteristics of millet cultivars, a split-split plot experiment was conducted in a randomized complete block design with three replications at the Research Farm of Isfahan University of Technology, in 2015. Two millet cultivars (Bastan and Pishahang) were planted in two sowing dates (June 22 and July 6) under two irrigation regimes (55% and 85% depletion levels of the available soil water) and two nitrogen fertilizer levels (available amount and 112.5 kg ha-1, nitrogen from Urea fertilizer, N= 45%). The results showed that drought stress caused a significant reduction in grain and biological yield by decreasing chlorophyll content, leaf area index, relative water content, membrane stability index, spade, quantum efficiency of photosystem II, height, panicle length and 100-seed weight. Nitrogen application resulted in significant increase in grain and biological yield in normal irrigation, but did not have a significant effect on yield in drought stress. In normal irrigation, grain and biologic yield were more in June 22 than July 6, while in drought stress condition, delay in sowing date resulted in improved yield compared to June 22. Bastan had lower electrolyte leakage and more membrane stability in drought stress. The results showed that delay in sowing date by modulating the temperature conditions could be a suitable solution for yield loss compensating under drought stress conditions, also under stress conditions, nitrogen application should be applied at a higher level to find out optimum amount of nitrogen use.
Nader Shahbazi, Seyyed Kamal Kazemitabar, Ghafar Kiani, Ali Pakdin Parizi , Pooyan Mehraban Joubani ,
Volume 10, Issue 45 (12-2021)
Abstract
By examining physiological reactions related to salinity tolerance among different landraces and cultivars, tolerant and sensitive cultivars can be selected and used in breeding programs and cultivation development of this important oilseed. For this purpose, physiological reactions in eight different sesame genotypes in a factorial experiment based on a completely randomized design with the main salinity factor due to mixing Caspian Sea water with normal water at 3 levels (control, 6 & 9 dSm-1) and sub-factor 8 Sesame genotype including Babol, Dashtestan 2, Oltan, Pakistani, tn-78-389 and tn-78-393, Halil, Naz single-branched genotypes in three replications in pots in the greenhouse of the Plant Breeding Department of Sari University of Agricultural Sciences and Natural Resources in the year 2020 were examined. The results of this experiment showed that at salinity levels of 6 and 9 dS, among the eight different genotypes of sesame studied, Oltan cultivar with the highest amount of total dry weight (8.42 & 5.27 gr), amino acid proline (10.35 & 15 μmol/g fresh leaf weight), membrane stability index ( 82.65 & 74.14%), relative water content (81.33 & 69.33%), leaf chlorophyll and carotenoid content (based on mg/g fresh weight), chlorophyll a (0.74 & 0.7), total chlorophyll (1.12 & 0.95) and carotenoids (0.41 & 0.46) and antioxidant enzymes activities (based on enzyme unit per gram fresh weight), superoxide dismutase (18.22 & 29.05), ascorbate peroxidase (14.9 & 19.21), catalase (1.51), guaiacol peroxidase (4.8 & 5.43), potassium concentration (6.6%), sodium to potassium concentration ratio index (0.06 & 0.12) and the lowest amount of sodium ion concentration (0.41 & 0.7) were more tolerant of stress compared to other cultivars. In contrast, Pakistani cultivar with the lowest amount of total dry weight (5.12 & 2.14 gr), amino acid proline (5.86 & 6.7 μmol/g fresh leaf weight), membrane stability index (66.31 & 57.86%), relative water content (58 & 48.33%), leaf chlorophyll and carotenoid content (based on mg / g fresh weight), chlorophyll a (0.46 & 0.37), total chlorophyll (0.69 & 0.5) and carotenoids (0.25 & 0.27) and Antioxidant enzymes activities (based on unit of enzyme per gram of fresh weight), superoxide dismutase (11.61 & 10.31), ascorbate-peroxidase (8.61 & 8.78), catalase (0.84), guaiacol peroxidase ( 1.77 & 2.16), potassium concentration (3.68%), sodium to potassium concentration ratio index (0.59 & 1.12) and the highest amount of sodium ion concentration (2.15 & 2.57) were the most sensitive cultivar in stress tolerance compared to other cultivars. Based on the evaluated traits and the response of the studied cultivars to salinity stress, oltan was identified as a tolerant cultivar and Pakistani as a salinity sensitive cultivar compared to the other cultivars. Therefore, these cultivars can be suggested for use in future breeding programs to introduce new cultivars.
Fatemeh Yazdanpanahi, Mohsen Sanikhani, Azizollah Kheiry, Hossein Rabbi Angourani,
Volume 13, Issue 62 (10-2024)
Abstract
Salinity is one of the most important environmental stresses that diminishes the growth and yield of crop plants. Application of biological fertilizers, especially amino acids, is important to overcome this issue in order to improve the growth, quantity and quality of the products. In order to investigate the effect of amino acids on some morphophysiological characteristics of the medicinal plant Dracocephalum moldavica grown under salinity stress, a factorial experiment was conducted in a randomized complete block design (RCBD) with three replications in the greenhouse of the University of Zanjan, during Autumn of 2022. The experimental treatments included three levels of salinity (0, 40 and 80 mM NaCl) as the main plots and phenylalanine (2 and 4 mM), gamma aminobutyric acid (GABA 5 and 10 mM) plus control were used as subplots. The results showed that with increasing salinity, some morphological and physiological parameters of the plant decreased, but the anthocyanin content of the flower increased. The use of 2 mM phenylalanine significantly increased the FW and DW of the plant, and the use of 4 mM led to a significant increase in the number of leaves, cell membrane stability index, chlorophyll and carotenoid at 0 mM and anthocyanin in the condition of 80 mM sodium chloride. Also, 5 and 10 mM GABA in non-stress conditions and 2 mM phenylalanine in 40 and 80 mM salinity conditions led to an increase in stem diameter. Although the application of 4 mM phenylalanine had the greatest effect in non-stress conditions, GABA 10 mM in salt stress conditions had a more effective role in increasing the FW and DW of the plant, chlorophyll, and carotenoid content, and as a result, increasing the stability of the cell membrane. Therefore, the use of different levels of the amino acids 4 mM phenylalanine and 10 mM GABA is recommended to improve the growth and development pattern and physiological characteristics of the Dracocephalum moldavica L. plant.
