Elham Yusefi Tanha, Seyfollah Fallah, Ali Taddayon,
Volume 4, Issue 13 (10-2015)
Abstract
In order to evaluate the effect of seed priming on some effective physiological parameters on seed germination of pea (Pisum sativum L.) under chilling stress, a factorial experiment was conducted in completely randomized design with four replications. Treatments were consisted of priming treatments (hydropriming, halopriming, osmopriming and no priming) and temperature (3, 6, 9, 12 and 15 ˚c). Results showed that chilling stress caused a linearly decline of germination rate. The maximum germination rate achieved with hydropriming treatment. Also, hydropriming treatment prevented the reduction of germination percentage at 3˚C with no significant difference with halopriming treatment. The maximum vigor index was observed for halopriming. Reduction of temperature resulted in proline and activity of catalase and guaiacol peroxidase enzymes increasing in all priming treatments. Polyethyleneglycol not only had no effect on seeds germination, but also reduced germination parameters of pea in some temperatures. In conclusion, hydropriming and halopriming treatments are suitable for physiological parameters, germination rate and percentage improvement of pea seeds under chilling stress conditions that they can provide establishment and better growth of this plant under cold stress. This method could be appropriate for increasing the rotational potential of this plant as green manure in cold and mild regions of the country.
Haniyeh Saadat, Mohammad Sedghi,
Volume 13, Issue 62 (10-2024)
Abstract
In order to investigate the effect of sodium nitroprusside pretreatment on the improvement of physiological traits and antioxidant enzymes in rice seedlings under salinity stress, a factorial experiment was conducted in a completely randomized design with three replications at Mohaghegh Ardabil University in 2023. Experimental treatments included four salinity levels (0, 50, 100, and 150 Mm) and sodium nitroprusside in three levels (control, 40 and 80 µm). The results showed that salinity of growth indices, including germination percentage (GP), germination rate (GR), Seedling Length Vigor Index (SLVI) and Seedling Weight Vigor Index (SWVI), reduced, but seed priming with different levels of sodium nitroprusside, especially the level of 80 µm, improved these traits. Salinity increased the Mean Germination Time (MGT), so that the highest MGT was observed at (0.371 days) sodium nitroprusside 40 µm and salinity of 150 mM. The Catalase (CAT) and superoxide dismutase (SOD) enzymes activity in pretreatment with sodium Nitroprusside 80 µm and a salinity of 150 mM were 37 and 40% higher than the control (distilled water) and without salinity, respectively. Salinity increased the activity of peroxidase enzyme, so that the lowest was observed at peroxidase enzyme (58.444 units mg-1 protein) in the control (without salinity). Also, the peroxidase activity in pretreatment with sodium nitroprusside 80 µm compared with the control (distilled water) showed an increase of about 14%. The results showed that seed treatment with sodium nitroprusside 80 µm improves rice seed germination and biochemical traits, it can reduce the harmful effects of salinity on some traits in rice seedlings and improve seedling growth.
