Rasoul Azarmi, Akbar Padash, Ali Ashraf Soltani Toularoud, Behrooz Esmaielpour,
Volume 12, Issue 53 (5-2023)
Abstract
The application of dicyandiamide (DCD) nitrification inhibitors in soil causes soil contamination for many years and has received much attention in recent decades due to its potential toxic effects on plants, but none. No action has been taken to control and eliminate contamination of nitrification inhibitors. In this study, DCD nitrification inhibitor at 4 concentrations (0, 5, 50 and 100 mg / kg) was added to the soil in a pot experiment with four replications. Greenhouse experiment was performed using factorial based on a completely randomized design to test the effect of symbiotic fungi (plants inoculated with P. indica, G. etunicatum and G. mosseae) on DCD plant toxicity. Lettuce (Lactuca sativa) cultivar Black was selected for this experiment because of its worldwide economic value. Then some properties of chlorophyll fluorescence such as minimum fluorescence (F0), maximum fluorescence (Fm) and maximum quantum efficiency of photosystem II (Fv / Fm) were measured. HPLC results showed the concentration of DCD nitrification inhibitor in lettuce leaves was very important. It was found that plants inoculated with symbiotic fungi had lower DCD levels than non-symbiotic plants. Plants treated with P. indica had 70 and 80% less DCD (50 and 100 mg / kg, respectively) compared to other plants.
