Showing 5 results for Acetic Acid
Maryam Dahajipour Heidarabadi, Mohammadreza Dehghani, Fatemeh Moradi Andoohjerdi, Khalil Malekzadeh, Fatemeh Soroush,
Volume 10, Issue 42 (5-2021)
Abstract
Salinity resistance is one of the main concerns in arid and semi-arid regions. In this study, the effect of acetic acid pretreatment in salinity stress was investigated on parameters such as relative water content, electrolyte leakage, proline, malondialdehyde, phenolic compounds, protein and photosynthetic pigments content, phenylalanine ammonialyase enzyme activity and expression of important enzymes in rosmarinic acid biosynthesis (phenylalanine ammonialyase and rosmarinic acid synthase) in hydroponic culture of Melissa officinalis L. The seedlings were grown in Hoagland’s solution were pre-treated with acetic acid (10 µM) and then salinity stress was done with NaCl (35 mM) for 11 days. The results indicated that electrolyte leakage percent and malondialdehyde content were decreased, whereas, proline and phenol content were increased in acetic acid pretreated plants in salinity stress. Also, photosynthetic pigments content showed less reduction in acid acetic pretreated plants compared to the control plants. Phenylalanine ammonia lyase activity and gene expression increased in salinity stress. Increasing in rosmarinic acid synthase gene expression resulted in increasing rosmarinic acid content. Also, the amount of rosmarinic acid had a positive and significant relationship with the activity and expression of key genes in its biosynthesis. The results suggest that the use of acetic acid pretreatment, by initiating support mechanisms such as decreasing in peroxidation of membrane lipids, increasing in proline, phenolic compound, activity and expression of genes involved in phenolic compound and photosynthetic pigments played an important role in salinity stress modulation.
Mehdi Khorshidi,
Volume 10, Issue 45 (12-2021)
Abstract
Stone fruits are valuable because of their high nutritions and therefore propagation of the same rootstocks is vital in horticulture. The aim of the present study was to investigate some concentrations of growth regulators such as benzylaminopurine (BAP), gibberellic acid (GA3), naphthalene acetic acid (NAA), indole butyric acid (IBA) in shoot germination and rooting of myrobalan 29c. This experiment was performed in a completely randomized block design with three replications (at least 5 samples per replication) in 14 experimental groups (G1-G14). In this study, MS culture medium and growth regulators of BAP (0, 0.5, 1 and 2 mg/L), GA3 (0, 0.1 and 0.2 mg/L), NAA (0 and 0.1 mg/L) were used in shoot generation. The results showed that shoot generation varied between 1.05 in G1 group to 8.27 in G6 group. The shoot length varied between 2.87 cm in G5 group to 4 cm in G6 group. The best shoot generation was observed at concentrations of 1 mg/L BAP, 0.1 mg/L NAA and 0.2 GA3 at 8.27 numbers. The 1/2 MS medium and growth regulators of IBA (0, 0.25, 0.5 and 1 mg/L) and NAA (0, 0.25, 0.5 and 1 mg/L) were used for rooting. The results showed that rooting varied between zero numbers in the G1 group to 7.6 numbers with 8.78 cm length in the G13 group. The best rooting was obtained at 1/2MS with 0.5 IBA and 0.25 NAA concentration with 7.6 root numbers.
Alireza Sanie Khatama, Somayeh Rastegar, Abdolhossein Aboutalebi Jahromi, Hamed Hassanzadeh Khankahdani, Seyed Ali Akbar Bagherian,
Volume 11, Issue 49 (8-2022)
Abstract
Cold stress is one of the abiotic stresses that leads to most losses in citrus. Mexican lime is one of the commercial citrus cultivars in the world, which is commonly used as a citrus rootstock in the southern regions of Iran. That is one of the most sensitive citrus cultivars to cold stress. In order to investigate the ameliorating effect of some bioactive compounds on chlorophyll fluorescence parameters and electrolyte leakage of Mexican lime seedlings under cold stress, a split-factorial experiment was conducted in the form of the randomized complete block design. The experimental factors included temperature (0 and -6 °C) as a main factor and foliar application of melatonin (0 and 500 μM), mannitol (0 and 50 μM) and acetic acid (0 and 15 μM) as sub-factors. According to the results, the combined application of melatonin + mannitol + and acetic acid, by improving the photosynthetic parameters, reduced the destructive effects of cold stress (0 and -6 ºC) on photosynthetic machinery in Mexican lime seedlings. The highest Area was observed in the treatment of melatonin + mannitol + acetic acid. The treatments of melatonin and melatonin + acetic acid had the highest values of Fv/Fm and Fv/F0 and the lowest values of F0/Fm at both temperatures compared to the control. In this study, the most appropriate parameters to identify the best treatments included Area (total area between initial fluorescence curve and maximal fluorescence), maximum quantum yield of PSII photochemistry (Fv/Fm), thermal dissipation quantum yield (F0/Fm), and an indicator of the activity of the oxygen-evolving complex on the donor side of PSII (Fv/F0). Therefore, the mentioned bioactive compounds, especially melatonin as well as acetic acid, are proposed to manage cold stress in Mexican lime seedlings.
Mehrnoush Eskandari Torbaghan, Gholam Hossein Khalili Torghabe,
Volume 12, Issue 55 (9-2023)
Abstract
The use of indigenous extremophilic bacteria with plant growth-promoting haloalkaliphilic properties will help to cope with biotic and abiotic stresses. The present study focused to investigate the effect of these bacteria to induce salinity resistance in almond rootstocks under soil salinity and alkalinity and compare it with non-stress conditions. The roots of 108 Garnem rootstocks in four different soils (2, 4, 8, and 16 dS m-1) were inoculated with two bacterial strains and sterilized control from three groups of halophilic, alkaliphilic, and haloalkaliphilic isolated from the rhizosphere of almond’s cultivation sites. Plant growth-promoting properties, including tri-indole acetic acid production, phosphate mineral dissolution, and exo-polysaccharide production, were measured for alkaliphilic isolates (213.33, 127.55, and 578.11 mg L-1), haloalkaliphilic isolates (77.13, 73.99, and 284.54 mg L-1), and halophilic isolates (15.98, 40.19 and 35.90 mg L-1, respectively). The inoculated plants with halophilic bacteria were found to better grow compared to other inoculated plants. In addition, these plants accumulated more chlorophyll, sugar, and proline. The root fresh and dry weights were maximum by the haloalkaliphilic bacteria inoculation. The alkaliphilic and halophilic bacteria also caused a higher increase in fresh and dry weights of shoots. As well, root and shoot moisture contents were the highest ones in the 16 and 8 dS m-1 salinity by haloalkaliphilic inoculation. In general, the application of haloalkaliphilic bacteria was found to have a better effect on root growth and halophilic bacteria in the morphological and physiological properties of almond rootstocks. Compared with 8 dS m-1 and other salinity levels, extremophile bacteria had a stronger effect on soil salinity and alkalinity mitigation for almond rootstocks at 16 dS m-1 salinity.
Ali Rezapour, Maryam Dehestani-Ardakani, Kazem Kamali, Jaber Nasiri, Heidar Meftahizadeh,
Volume 13, Issue 59 (4-2024)
Abstract
This research was conducted to optimize in vitro micropropagation of Begonia rex as an important ornamental pot plant. In vitro callogenesis and regeneration of three cultivars of B. rex (Jurassic, Silver dollar and Silver king) were carried out starting from leaf segments as explants. Eight Murashige and Skoog′s media consisted of four plant growth regulators, including α-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), 3-Indole-3-butyric acid (IBA), and Thidiazuron (TDZ). After four weeks, callogenesis and regeneration started. After eight weeks of proliferation, the explants were transferred to MS culture medium supplemented with 0.05 mg/l gibberellic acid. The highest direct regeneration percent occurred in the medium containing 0.5 mg/L BA + 0.2 mg/L TDZ. Callogenesis, regeneration and root formation occurred in all treatments. The highest number of leaves was observed in the culture medium supplemented with 1 mg/L IBA + 1 mg/L BA in the Jurassic cultivar. Medium fortified with 0.2 mg/L NAA + 0.2 mg/L BA and 1 mg/L IBA + 1 mg/L BA was selected as the best medium for increasing the fresh weight and proliferation of cv. Jurassic. The highest amounts of chlorophyll a and b were obtained in 0.5 mg/l BA + 0.2 mg/l TDZ and 1 mg/l of IBA + 1 mg/l of BA treatments in ‘Jurassic’ and ‘Silver dollar’ cultivars. The survival rate of transplanted plant lets was about 100%. Generally, the combination of 1 mg/l IBA with 1 mg/l BA could be introduced as an effective and useful treatment in the micropropagation of studied three cultivars of Begonia rex.
