Zeynab Vosoo Abdollah, Saied Navabpour, Hourieh Najafi, Seyed Mojtabaa Mollaeii,
Volume 13, Issue 63 (vol. 13, no. 63 2024)
Abstract
Drought is one of the most important non-living stresses in the production of agricultural products, which causes great damage to crops, especially wheat, every year. Drought resistance is a complex trait that is controlled by multiple genes and is a proof of the complexity of improving this important agricultural trait. This study was conducted in order to investigate the effect of different levels of drought stress on some biochemical traits and enzyme changes and the expression level of some genes involved in the response to drought stress in wheat cultivars at the research station in Korkuy city. The seeds of the studied wheat cultivars were cultivated under field conditions after disinfection. The experiment was conducted as a split plot in the form of a randomized complete block design with three replications. The main factor of drought treatment included -0.3 times (agronomical capacity as a control treatment), -2 times and -4 times and the secondary factor included wheat cultivars Kalateh, Baharan and Gonbad. In order to evaluate the expression pattern of studied genes including P5CS, CAT, SOD, TaNAC2a, BADH, iQ5 device of Bio Rad Company was used. The results of quantitative real time PCR analysis showed that the expression levels of SOD, P5CS, BADH, CAT and TaNAC genes changed significantly in response to drought stress. Also, the amount of chlorophyll a and b decreased significantly under drought stress and the amount of ascorbic peroxidase enzyme increased significantly. That drought stress led to an increase in the expression of genes involved in this stress, which included SOD, P5CS, BADH, CAT, and TaNAC2A. According to the analysis results of this study, it can be concluded that the kalateh cultivar had the highest antioxidant enzyme activity, which indicated the superiority of this cultivar over other cultivars.
