Showing 3 results for Taghizadeh
Mina Taghizadeh, , , ,
Volume 8, Issue 32 (vol. 8, no. 32. 2020 2019)
Abstract
In vitro mutagenesis has been applied for the selection of plants tolerant to stress including heavy metal stress. The objective of the present study was to induce in vitro mutation on increasing tolerability and remediation evaluation using EMS in calli of Spartium junceum L. In the first experiment, calli initiated were exposed to cadmium. Result showed that the callus growth parameters decreased with increasing Cd concentrations in medium. In presence of Cd, contents of secondary metabolites including total phenolics, flavonoids, and flavonols, increased compared with control. Cd accumulation in callus increased with increasing Cd concentrations. The highest Cd accumulation was found 791.33 mg Cd kg-1 of dry weight at 40 mgL-1 Cd. The In the second experiment, calli were treated with 0.2% EMS for 10 minutes. Afterward, the treated calli were sub-cultured to medium supplemented with 0 and 10 mgL-1 Cd. Results showed that the growth of the calli treated by EMS decreased but the activities of non- enzymatic antioxidant compared with control. The application EMS was not increased Cd accumulation while tolerance index calli of Spartium junceum.
Mina Taghizadeh, Fahimeh Sadat Sajadi, Babak Valizadeh Kaji,
Volume 9, Issue 35 (vol. 9, no. 35 2020)
Abstract
Considering the high water requirement of grass during growth stages and the limitation of irrigation sources, it is important to find a suitable way to reduce the water requirement of the lawns as cover plants used in the Landscape. The purpose of this study was to investigate the effect of 2,4,5-T on the induction of callus from the hypocotyl explant in Kentucky bluegrass, induce in vitro drought stress using polyethylene glycol and evaluate the tolerance to stress using folic acid in in vitro conditions in Kentucky bluegrass. In the first experiment, the effect of 2,4,5-T in concentrations of 0.5, 1 and 2 mg / L in combination with two concentrations of 0 and 0.1 mg/l BA was investigated and highest percentage of callus induction at a concentration of 0.5 mg/ of 2,4,5-T alone was observed. In the second experiment, different concentrations of PEG were used for induction of drought stress in calli. Results showed that increasing the concentration of PEG decreased the growth indices in callus, so that in the concentration of 3 and 4 percent PEG, the least amount of measured traits of callus was observed. In the third and fourth experiments, folic acid was used to suppress drought stress induced by PEG in two stages of callus and regeneratin plants. The results of the third experiment showed that the use of folic acid in MS medium at 0.5 and 1 mg /l concentration reduced callus growth traits and increased soluble sugar content. In the fourth experiment, the results showed a decrease in the measured traits of regenerated plants in 4% PEG. The highest amount of soluble sugars in regenerated plants was observed in treatment 1 mg / l folic acid in combination with 0 % PEG.
Fatemeh Mojdehi, Mina Taghizadeh, Amir Hossein Baghaie, Mahdi Changizi, Shahab Khaghani,
Volume 11, Issue 47 (Vol. 11, No. 47 2023)
Abstract
One of the methods of remediation and cleaning of soils contaminated with heavy metals is the use of organic compounds such as vermicompost to purify and stabilize the contamination. In this study, the effect of vermicompost on the uptake of heavy metals including lead and cadmium by ornamental sunflower and the effect of these metals on germination and plant establishment characteristics were investigated. This study was carried out in two experiments of germination and greenhouse in 1397-98 in Islamic Azad University, Arak Branch in a factorial way in a completely randomized design. In the first experiment, concentrations of 50, 100 and 200 mg / l of lead nitrate and cadmium chloride were used and then germination indices were evaluated. In the second experiment, concentrations of 0, 5, 10 and 15 mg/kg of lead metal, concentrations of 0, 400 and 600 mg/kg of cadmium and three levels of 0, %1 and %2 of vermicompost to the ability of uptaking heavy metals were used by ornamental sunflowers. The results of the first experiment showed that increasing the concentrations of heavy metals lead and cadmium reduced the length of root, shoot and germination indicators such as seed vigor index, germination rate index and average germination rate. The results of the second experiment showed that vegetative growth of ornamental sunflower was not affected by increasing the concentration of lead and cadmium in the soil, but the content of lead and cadmium in the soil, lead and cadmium in the roots and lead and cadmium in the stem increased. The study indicated that H. annuus "Sungold" plant showed tolerance to lead and cadmium contamination and we recommend its ability for the remediation of lead and cadmium contaminated soils. Also, the activity of the enzyme superoxide dismutase increased as the concentration of heavy metals in the culture medium increased. Vermicompost implementation was able to partially improve the negative effects of these heavy metals. With increasing the concentration of heavy metals, the activity of superoxide dismutase increased by 30-50% compared to the control. Vermicompost reduced the amount of these metals in the soil, roots and stems of the ornamental sunflower plant, and this ornamental plant can be introduced as a purifier of lead and cadmium contaminated areas.
