Moslem Madadi, Saeid Khomari, Ahmad Javadi, Omid Sofalian,
Volume 5, Issue 15 (Vol. 5, No. 15, 2016 2016)
Abstract
The varying seed priming methods have widely been used for the improving germinability and seedling establishment of numerous economic plants especially under environmental stresses. In this context, in order to evaluate the advantage of priming on different physiological and biochemical indices of black cumin seed germination and seedling growth in saline conditions, the factorial experiment was conducted based on RCB design with four replicates in the faculty of agricultural science laboratory complex. Experimental factors included five priming methods (non-priming control, priming with calcium nitrate, nano zinc oxide, calcium nitrate+nano zinc oxide and hydro-priming) and three salinity levels (0, 50 and 100 mM NaCl). The results of the present research indicated that despite the inhibitory effect of salinity on black cumin seed germination and seedling growth characteristics, seed pre-treatment substantially alleviated the deleterious impacts of salt due to enhancement of prescribed parameters. When NaCl concentration increased in germination medium, total protein content of seedling was reduced and catalase activity was upregulated, while seed priming caused increase of the both traits under salinity. Among the applied priming methods, seed pre-treatment with calcium nitrate+nano zinc oxide had the highest efficacy and the best performance in ameliorating inhibition of measured parameters of black cumin induced by salt.
Parisa Sheikhnavaz Jahed, Mohammad Sedghi, Raouf Seyed Sharifi, Omid Sofalian,
Volume 11, Issue 50 (vol. 11, no. 50 2022)
Abstract
In order to investigate the effect of priming on germination characteristics of aged pumpkin seeds, under salinity stress an experiment was performed as a factorial design in a completely randomized design with 4 replications. Experiment was done in the Seed Science and Technology Laboratory of the Faculty of Agriculture and Natural Resources, Mohaghegh Ardabili University. Treatments included seed deterioration (control, 85% and 75%), salinity stress (zero, 75 and 150 mM), and priming (control, hydropriming, gibberellin, Gr24, benzyl-aminopurine, and spermidine). The results showed that salinity stress and seed deterioration reduced germination percentage, root length, stem length, relative water content (RWC), protein and carotenoids so that salinity treatment of 150 mmol, degree of 75 % seed deterioration showed the lowest percentage of these traits. Also, the activity of CAT, POX and SOD due to seed deterioration was 75% lower than other treatments. While priming with spermidine was able to significantly increase the amount of these enzymes in conditions of high salinity MDA, which is one of the most important results of stress, decreased under salinity stress and aging, but seed priming decreased MDA sharply, especially in priming with spermidine. In addition, seed priming was able to significantly reduce the effects of salinity stress and seed deterioration so that the percentage of germination and protein in increased gibberellin priming, root and shoot length in priming with Gr24, as well, and RWC and carotenoids in priming Spermidine. Salinity stress applied on deteriorated seeds led to disruption and reduction in physiological traits, while seed priming by different methods led to a reduction and improvement of adverse stresses.
