Showing 5 results for Modarres
Masoomeh Modarres, Mehrdad Lahooti. , Javad Asili, Mohammad Kafi., Ali Ramezani,
Volume 2, Issue 4 (Vol. 2, No. 4, year 2013 2013)
Abstract
Salvia leriifolia is a medicinal plant from Lamiaceae family native to Khorasan and Semnan Provinaces. This plant has medicinal properties such as antinociceptive, anti-inflammatory, antidiabetic, antimicrobial and Antioxidant which all have been confirmed by researchers. In this research, callus induction was established for production of phenolic acids. For this purpose, the leaf explants were cultured in MS medium supplemented with 2,4-D (0,1,2,3,4 mgL-1), KIN (0,0.3,1mgL-1), BAP (0,1,2,3,4,5,6 mgL-1) and NAA (0,1,2,3 mgL-1). Accumulation of phenolic acids was measured after 4 weeks of culture by HPLC. Results showed that the best treatments for callus induction and growth were at the BAP5 mgl-1+NAA5mgl-1 and 2, 4- D 3 mgl-1 + KIN1mgl-1. Accumulation of caffeic acid and salvianolic acid B in calli was higher than leaves of Salvia leriifolias. The highest rosmarinic acid concentration was found in the treatment BAP 5mgl-1+NAA5mgl-1 that were about 3 times higher than in leaves.
Masoomeh Modarres, Ziba Ghasimi Hagh,
Volume 8, Issue 29 (vol. 8, no. 29 2019)
Abstract
Abstract:
Salvia leriifolia is a medicinal plant belonging to lamiaceae family. In this study, the influence of light, phenylalanine and caffeic acid on cell growth and accumulations of phenolic acids were investigated. For this propose, the leaf explants were cultured in MS medium supplemented with BAP and NAA for callus induction. A cell suspension culture of S. leriifolia derived from leaf -derived callus was established in liquid MS medium with the same hormonal content. The biomass and phenolic acids content were analyzed after 15 days of the culture. Quantitative analyses of the phenolic acids in ethanolic extracts from the biomass were conducted by applying the HPLC method. Both dry weight and phenolic acid content were increased by light irradiation. Addition of 10 mg/l phenylalanine enhanced accumulation of caffeic acid (4fold),rosmarinic acd(4fold) and Salvianolic acid(1.6fold) compared to control. Addition of 10 mg/l caffeic acid decreased cell growth and accumulation of phenolic acids. This study will help us understand how secondary metabolism in Salvia leriifolia responds to precursors and light provide a reference for the improvement of the production of targeted compounds in the near future.
1msc Student Somayyeh Jokar, Dr Ziba Ghasimi Hagh, Dr Hojatollah Bodaghi, Dr Masoomeh Modarres,
Volume 8, Issue 32 (vol. 8, no. 32. 2020 2019)
Abstract
Salvia leriifolia Benth. is one of the most important medicinal plant so, the purpose of the present study is firstly improve callus production with root and leaf explants in combination of NAA and 2.4-D with Kin in MS medium. The results showed that NAA was not produced callus in the leaf and root explant, while with 2.4-D, the most suitable callus was obtained from the leaf explant at a concentration of 1 mg/ lit Kin with 2 mg/ lit 2,4-D. Then, effect of UV-A and UV-B radiation as elicitors in two times (30 and 60 min) on content of phenolic, flavonoid, rosmarinic acid and caffeic acid in callus of Norouzk plant was investigated. The results showed that UV-A and UV-B radation increased content of total phenol, flavonoids, rosmarine acid and caffeic acid in callus. The highest fresh weight, phenol and flavonoid content were observed in 30 minutes UV-A treatment and the highest accumulation of rosmarinic acid and caffeic acid was observed in the calluses treated with 30 minutes UV-B radiation. Therefore, by optimizing the uv ellicetor, it is possible to produce the desired secondary metabolites like as caffeic acid of salvia in callus culture.
Ali Kazempour, Younes Sharghi, Sayed Ali Mohammad Modarres Sanavi, Hossein Zahedi, Fatme Sefid Kon,
Volume 12, Issue 53 (vol. 12, no. 53 2023)
Abstract
In order to study foliar application of amino acids on morphophysiological properties and thyme essential oil under different irrigation regimes, an experiment was conducted as a split plot based on a randomized complete block design with three replications in the Research Farm of Tarbiat Modares University Faculty of Agriculture in the year 2020 crop season. Experimental factors were irrigation regimes at three levels including normal irrigation, moderate water stress, severe water stress (withholding irrigation until 25%, 45% and 65% of soil available water depletion in the root zone respectively and then irrigation up to field capacity from plant establishment to harvest time), were randomized to the main plot units and spraying at 6 kinds (aspartic acid, tyrosine, proline, commercial amino acids, distilled water and without spraying) were located in subplot units. The results showed that with reduction of irrigation to the level of severe water stress, plant height, fresh and dry weight of leaves, leaf area, total chlorophyll, percentage of essential oil decreased, and glycol peroxidase and proline showed a significant increase compared to optimal irrigation. Catalase activity also increased under moderate water stress and decreased under severe water stress. Foliar application of amino acids had a positive additive effect on the studied traits. The highest essential oil yield (about 11.20 kg/ha) was assigned to plants under commercial and proline amino acid foliar application under full irrigation conditions. Based on the general results of proline foliar application, increasing the concentration of osmolites such as proline under water stress conditions was able to help the plant withstand drought stress by helping to maintain osmotic pressure and compensate for part of the reduction in growth and dry weight. Therefore, consumption of this type of amino acid under stress conditions is recommended to reduce the effects of drought stress. Under non-stress conditions, foliar application of this type of amino acid had the greatest effect on plant growth and yield.
Seyed Jaber Hosseini, Zeinolabedin Tahmasebi-Sarvestani, Hematolahe Pirdashti, Seyed Ali Mohammad Modarres-Sanavi, Ali Mokhtassi-Bidgoli, Saeid Hazrati, Mahsa Rafati Alashti,
Volume 12, Issue 57 (vol. 12, no. 57 2023)
Abstract
Obtaining a high dry matter yield is one of the most important goals of plant physiologists. In this regard, it is necessary to know the effective traits in the production of plant dry matter. Salinity stress is also one of the most important non-biological stresses that limits plant growth and affects the morphological, physiological and biochemical properties of plants. Therefore, in order to understand the relationship between dry matter yield and antioxidant properties, photosynthetic and non-photosynthetic pigments of mint under salinity stress, an experiment was conducted as a factorial split-plot (18 mint ecotypes, including longifolia, pulegium, spicata, rotundifolia, mozafariani and piperita, salinity stress at control, 2.5, 5 and 7.5 dS/m levels and harvest stage at 2 stages), based on a randomized complete block design with four replications. The results showed that salinity stress affected the correlation between dry matter and other studied traits. Also, path analysis at different levels of salinity stress showed that total chlorophyll traits had a very high direct effect on dry matter yield. On the other hand, at a 7.5 dS/m salinity level, the dry matter yield of the plant depended on a greater number of traits compared to lower-salinity stress levels. Also, the correlation between traits in the first and second harvest stages was different. The anthocyanin pigment trait had a positive and significant correlation with dry matter yield at 7.5 dS/m. The indirect role of carotenoid pigment was also evident in severe stress. Based on path analysis, all the studied traits, including non-enzymatic antioxidants and anthocyanin and carotenoid pigments, were strongly dependent on the total chlorophyll trait to affect the dry matter yield.
