Showing 6 results for Kheiry
Mohsen Sanikhani, Arezoo Akbari, Azizollah Kheiry,
Volume 9, Issue 35 (vol. 9, no. 35 2020)
Abstract
The medicinal plant bitter apple belongs to Cucurbitaceae family. To study the effect of phenylalanine and tryptophan amino acids on morphological and physiological characteristics of Citrullus colocynthis L., an investigation was conducted according to a completely randomized block design with four treatments and three replications in University of Zanjan, Iran. The studied factors were consisted of different levels of phenylalanine and tryptophan (0, 0.5, 1, 2 mM). According to the results, phenylalanine and tryptophan very significantly affected on the majority of studied traits including plant length, fresh and dry weight of foliage, seed yield, weigh of 1000 seeds, chlorophyll, total phenol and flavonoid contents (p≤ 0.01). The maximum content of total phenol and flavonoid were reached to 16.94 and 7.31 mg/100 g fresh weight in the concentration of 1 mM phenylalanine compared to control with 10.72 and 4.04 mg/ 100 g fresh weight respectively. Also, yield of fruit and antioxidant activity were affected significantly by the amino acid treatments (p≤ 0.05). The maximum fruit yield was 8930.6 kg/ha at the concentration of 0.05 mM tryptophan compared to 6344.4 kg/ha in control. In conclusion, various levels of tryptophan and phenylalanine can effectively improve morphological and physioloogical characteristics and hence production of this crop. To achieve the best performance, application of 2 mM phenylalanine for maximum yield of foliage, 1 mM of the two amino acids for phytochemical characteristics and 0.5 mM of the two amino acids for fruit yield, depending on the aim, can be recommended.
Mohsen Sanikhani, Arefeh Rastgoo, Azizollah Kheiry, Mitra Aelaei,
Volume 9, Issue 36 (vol. 9, no. 36 2020)
Abstract
To study the effect of foliar application of proline and salicylic acid on steviol glycosides and phenolic of Stevia, an experiment was conducted based on a completely randomized block design in a factorial arrangement with three replications in the University of Zanjan. Foliar application of proline in three levels (0, 1, 2 mM) as well as salicylic acid (0, 0.3, 0.6 mM) were applied after establishment of transplants in the field three time with 10 days intervals. Results showed that salicylic acid and proline applications significantly influenced on steviol glycosides, phenol and flavonoid. The treatment with salicylic acid had positive effects on all steviol glycosides, phenol and flavonoid, but proline only enhanced contents of glycosides stevioside, rebaudioside A, phenol and flavonoid. The highest average steviol glycosides stevioside (60.32 mg/g dw), rebaudioside A (24.14 mg/g dw) and total phenol (19.94 mg/g dw) was recorded in 1 mM proline in combination with 0.6 mM salicylic acid. The highest average flavonoid (10.02 mg/g dw), in 0.6 mM salicylic acid, and lowest average steviol glycosides and phenolic contents observed in control. Collectively, the results showed that application of proline and salicylic acid significantly improved production of active substances in Stevia rebaudiana.
Azizollah Kheiry, Mohadseh Zarandood, Allolo Afsoon Rezaie, Taher Barzgar, Mitra Aelaei,
Volume 9, Issue 38 (vol. 9, no. 38 2020)
Abstract
In order to study the effect of nitroxin bio-fertilizers (0, 3 and 6 liters per hectare) and ammonium sulfate fertilizer (0, 75 and 150 kg.ha-1) on yield, phytochemical, phenotypic and trace elements of Marrubium vulgare L., a factorial experiment based on completely randomized block design with three replications was conducted at Zanjan University in 2017. Nitroxin bio-fertilizer and ammonium sulfate had a significant effect on yield and mineral yield index. The highest weight of air part organs (157 g), inflorescence (349), inflorescence flowers (11.53 cm), total chlorophyll (12 mg.g-1), Phenol (112 mg.g-1), antioxidant (99.26) and tannin (35.66 mg.g-1) in combined treatments of 3 liters per hectare of nitroxin with 150 kg/ha of ammonium sulfate and 6 liters of nitroxin treatment with 75 kg/ha of ammonium Sulfate and the highest amount of flavonoid (12.96 mg.g-1) with 6 liters per hectare of nitroxin, with 150 kg ha-1, ammonium sulfate and anthocyanin (11.12 mg.g-1), was obtained in 6 liters/ha nitroxin. In conclusion, the results showed that biofertilizer consumption of nitroxin and ammonium sulfate, as compared to control, could be effective in increasing economic performance and qualitative characteristics of plant growth.
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Afsoon Rezaie Allolo, Mohsen Sanikhani, Azizollah Kheiry, Maliheh Yaghoobi,
Volume 13, Issue 60 (vol. 13, no. 60 2024)
Abstract
In order to investigate the effect of foliar application of different levels of ethanol and methanol on the biochemical characteristics of Digitalis purpurea L., an experiment was conducted based on a completely randomized design with three replications at the University of Zanjan research greenhouse. The treatments consisted of two levels of ethanol (5 and 10%) and methanol (5 and 10%), along with a control treatment (distilled water). The results showed that the maximum amount of total chlorophyll (1.78 mg/g), total carotenoid (0.10 mg/g), total phenol (15.35 mg/g), total flavonoid (58. 2 mg/g) and antioxidant activity (43.76%) were obtained under a 10% methanol treatment. The highest amounts of proline (4.47 mg), soluble sugar (76.20 mg/g), relative water content (76.02%) and leaf nitrogen (1.61%) were observed under a 10% ethanol treatment. The ion leakage index (15.21%) with foliar application of 10% ethanol and catalase enzyme (1.66 mg/g protein) and peroxidase enzyme (0.34 mg/g protein) activity showed a decrease with foliar application of 10% methanol. According to the results, the application of different levels of hydroalcohols improved the biochemical properties of Digitalis purpurea L.
Fatemeh Yazdanpanahi, Mohsen Sanikhani, Azizollah Kheiry, Hossein Rabbi Angourani,
Volume 13, Issue 62 (vol. 13, no. 62 2024)
Abstract
Salinity is one of the most important environmental stresses that diminishes the growth and yield of crop plants. Application of biological fertilizers, especially amino acids, is important to overcome this issue in order to improve the growth, quantity and quality of the products. In order to investigate the effect of amino acids on some morphophysiological characteristics of the medicinal plant Dracocephalum moldavica grown under salinity stress, a factorial experiment was conducted in a randomized complete block design (RCBD) with three replications in the greenhouse of the University of Zanjan, during Autumn of 2022. The experimental treatments included three levels of salinity (0, 40 and 80 mM NaCl) as the main plots and phenylalanine (2 and 4 mM), gamma aminobutyric acid (GABA 5 and 10 mM) plus control were used as subplots. The results showed that with increasing salinity, some morphological and physiological parameters of the plant decreased, but the anthocyanin content of the flower increased. The use of 2 mM phenylalanine significantly increased the FW and DW of the plant, and the use of 4 mM led to a significant increase in the number of leaves, cell membrane stability index, chlorophyll and carotenoid at 0 mM and anthocyanin in the condition of 80 mM sodium chloride. Also, 5 and 10 mM GABA in non-stress conditions and 2 mM phenylalanine in 40 and 80 mM salinity conditions led to an increase in stem diameter. Although the application of 4 mM phenylalanine had the greatest effect in non-stress conditions, GABA 10 mM in salt stress conditions had a more effective role in increasing the FW and DW of the plant, chlorophyll, and carotenoid content, and as a result, increasing the stability of the cell membrane. Therefore, the use of different levels of the amino acids 4 mM phenylalanine and 10 mM GABA is recommended to improve the growth and development pattern and physiological characteristics of the Dracocephalum moldavica L. plant.
Afsoon Rezaie Allolo, Azizalah Kheiry, Mohsen Sani Khani, Maliheh Yaghoobi,
Volume 13, Issue 63 (vol. 13, no. 63 2024)
Abstract
In order to investigate the effect of foliar application of different levels of benzyl-adenine and glutamic acid on the concentration of elements and some physiological characteristics of Digitalis purpurea L. in one-year and two-year-old plants, a factorial experiment in the form of a completely randomized design with three replications was conducted at the University of Zanjan research greenhouse. The treatments consisted of the effect of plant age (one-year plants and two-year old plants) as the main factor and two levels of benzyl-adenine (0.5 and 1 mM) and two levels of glutamic acid (1 and 2 mM) with the control as a subfactor. The results showed that the highest amount of total nitrogen (1.70%), phosphorus (0.08%), and proline (3.86 mg/g) in the one-year-old plants and the highest amount of leaf magnesium (0.64 µg/Kg) and Soluble sugar (63.26 mg/g) were obtained in the two-year-old plants under foliar application of 1 mM benzyl-adenine. The maximum amount of elements potassium (1.90%), calcium (2.92%), and iron (152.21 µg/Kg) was obtained in two-year-old plants treated with 0.5 mM benzyl adenine. The lowest amount of ion leakage index of leaves (16.31%) and the highest amount of relative water content (89.61%) were recorded with foliar application of 2 mM glutamic acid in one-year-old plants, and the highest amount of catalase enzyme activity (154.4 mg/g protein) and peroxidase (1.61 mg/g protein) were obtained by foliar application of 1 mM glutamic acid in the two-year-old plants. The highest leaf fresh weight (123.51 g) and leaf dry weight (47.93 g) were obtained in the one-year-old plants with foliar application of 1 mM glutamic acid. According to the results, the application of different levels of benzyl-adenine and glutamic acid is recommended to improve the absorption of elements and also to improve the morpho-physiological characteristics of the Digitalis purpurea L.
