Showing 9 results for Bakhshi
Ali Momenpour, Ali Imani, Davod Bakhshi, Hamed Rezaei,
Volume 3, Issue 10 (3-2015)
Abstract
In order to evaluate the tolerance of some almond genotypes to salinity a pot experiment was carried out with 3 factors cultivar in 11 levels including Touno, Nonparaeil, Mamaie, Shokofeh, Sahand, Fragness, 1- 16, 1-25, A200,13-40 and GF677 and salinity in five levels including 0, 1.2, 2.4, 3.6 and 4.8 g/l of sodium chloride with electrical conductivity 0.5, 2.5, 4.9, 7.3 and 9.8 ds/m, respectively during 30, 60 and 90 days in 2013. The results showed that salinity stress affected the young trees through increasing the amount of minimum fluorescence (FO) and decreasing the maximum fluorescence (Fm) and reducing variable fluorescence (Fv) as well as the ratio of variable fluorescence to maximum fluorescence from 0.83 in the control plants to 0.72 in the upper leaves and 0.70 in the bottom leaves of Sahand and GF677. The result of morphological characteristics showed that GF677 was tolerated to water salinity up to 4.9 ds/m however, higher range of salinity had negative impact on the above factors. Also, the results revealed that salinity stress duration is a key factor causeing several visible damages. Overall, according to morphological characteristics and leaves visual damages and chlorophyll fluorescence parameters, Feragness and Sahand cultivars were the most tolerant and sensitive genotypes to salinity stress, respectively.
Roghayyeh Mokhberi, Ayatollah Rezaei, Alaedin Kordenaeej, Gholamreza Bakhshi Khaniki,
Volume 5, Issue 17 (Vol. 5, No. 17, year 2016 2016)
Abstract
Manipulating cell cultures by elicitors is one of the important strategies for induction of valuable metabolites in plant biotechnology. Therefore, in this study the effect of ultrasound and salinity on growth, primary and secondary metabolite production was studied in Dunaliella salina microalgae cell culture. Cell cultures were treated with sodium chloride salt at concentrations of 2 and 3 M and ultrasound (40 kHz) for the 2.5, 5 and 10 minutes in a completely randomized design with three replications. The evaluated parameters were cell growth, total protein content, photosynthesis pigments (chlorophyll a and b, total chlorophyll and carotenoids), antioxidant potential, membrane lipid peroxidation, amount of phenolic compounds, flavonoids, anthocyanins, soluble sugars, &beta-carotene and glycerol. The results showed that with increasing duration of exposure to ultrasound and salt concentration the cell growth and photosynthetic pigments were declined. In contrast, total protein, antioxidant potential, membrane lipid peroxidation, the amount of phenolic compounds, flavonoids, anthocyanins, &beta-carotene and glycerol were increased. The highest amount of &beta-carotene was achieved in the case of individual treatment of ultrasound irradiation at 10 min, 3 M salinity and combination of the two factors, with values of 12.3, 11.9 and 14.8 mg/l, respectively. The highest content of glycerol was measured in 3 M salinity, 10 min ultrasound irradiation and combined treatment of the two factors, 11.7, 13.5 and 16.8 mg/l, respectively. It appears that ultrasound potentiated the salinity effect and induced defense responses, secondary metabolism and so increased the amount of cells &beta-carotene and glycerol.
Mr Nasrollah Soori, Dr Davood Bakhshi, Dr Abdolhossein Rezaei Nejad, Mohammad Faizian,
Volume 8, Issue 30 (vol. 8, no. 30 2019)
Abstract
In order to evaluate the salinity tolerance of six pomegranate genotypes including Malas Dane Ghermez Esfahan, Malas Yazdi, Shirin Shahvar, Meykhosh Yazd, Malas Saveh and Malas Yousofkhani, a greenhouse experiment was conducted as a factorial based on randomized complete block design with five salinity levels of 0, 25, 50, 75 and 100 mM sodium chloride at Agricultural College of Lorestan University in 2015. The results showed that with increasing salinity, photosynthesis rate, transpiration rate, stomatal conductance, leaf relative water content, relative chlorophyll content and mesophilic conduction (carboxylation efficiency) significantly decreased, and the degree of intercellular CO2 concentration and leaf water use efficiency increased significantly. Also, comparison of meanings showed that there was a significant difference between genotypes in measured factors. Finally, the results of research showed that the genotype of Malas Yousofkhani, due to its superiority in most traits related to salinity tolerance, has more tolerant than other genotypes to salinity induced by sodium chloride.
Mr Nasrollah Soori, Dr Davood Bakhshi, Dr Abdolhossein Rezaei Nejad, دکتر Mohammad Faizian,
Volume 8, Issue 32 (vol. 8, no. 32. 2020 2019)
Abstract
Water and soil salinity are one of the most important restrictions on the growth and production of fruit trees. Control of this phenomenon can be one of the factors to managing the production and guarantee the production sustainability and optimal use of land and water. Therefore, in order to investigate the effect of salinity stress on the physiological and biochemical properties of some Iranian commercial pomegranate cultivars and identification of salinity tolerant cultivars, a greenhouse research conducted at the Faculty of Agriculture of Lorestan University in 2015. The Factorial experiment based on randomized complete block design with five levels of salinity (0, 25, 50, 75 and 100 mM NaCl) and six pomegranate cultivars include Malas Dane Ghermez Esfahan, Malas Yazdi, Shirin Shahvar, Meykhosh Yazd, Malas Saveh and Malas Yousofkhani and with three replications. After three months of applying salt stress, some physiological and biochemical properties of the plants were examined and measured. The results showed that in control treatment, compared to 100 mM NaCl treatment with increasing salinity, chlorophyll a (42%), chlorophyll b (40%), total chlorophyll (42%) and total carotenoids (65%) decreased significantly and Electrolyte leakage rate (27%), malondialdehyde (94%), and peroxidase (506%), catalase (478%), and ascorbate peroxidase (366%) enzymes increased. In addition, comparison of data meanings showed that there was a significant difference between cultivars in measured factors. The results showed that, in salt stress conditions, Malas Yousofkhani cultivar was superior to salinity tolerance in most traits, and this genotype was more tolerant to salinity than other evaluated cultivars.
Fatemeh Shahinfar, Sara Taghikhah-Khomami, Seyedeh Fatemeh Fallah, Mansour Afshar-Mohammadian, Davod Bakhshi,
Volume 10, Issue 42 (vol. 10, no. 42 2021)
Abstract
Saffron (Crocus sp.), is widely used in the food industry and is used as a flavoring and natural coloring agent. A comparative evaluation of the antioxidant activity in the stamen, as well as the effective and medicinal material of crocin in stigma in the agronomic species of saffron (C. sativus) in comparison with the native wild species of C. speciosus and C. caspius collected from Guilan province, were the main objectives of this study. The results of this study showed that the medicinal material of crocin in the stigma of C. speciosus and C. caspius with a significant difference was more than C. sativus, but the amount of crocin in the stamen of C. sativus with a significant difference was more than C. speciosus and C. caspius. The results of the present study also showed that the amount of total phenol and also the amount of the antioxidant activity (DPPH) in the stamen of C. sativus was significantly higher than the two species of C. speciosus and C. caspius, but the amount of flavonol in C. speciosus and C. caspius was significantly higher than C. sativus. Due to the fact that until now, the saffron stamen was considered as unusable part of saffron, according to the results of this study, the high level of antioxidant activity as well as the medicinal composition of crocin in the stamen of saffron, in addition to stigma, in the agronomic and wild species of saffron, this part of saffron has significant potential as a rich source of antioxidants, enriching people's food baskets and in a variety of pharmaceutical and food programs.
Shiva Rezaei, Mansour Afshar-Mohammadian, Davood Bakhshi, Seyedeh Fatemeh Fallah,
Volume 11, Issue 49 (vol. 11, no. 48 2022)
Abstract
Oleuropein, Hydroxytyrosol and Tyrosol are three major phenolic compounds present in olive with remarkable pharmacological effects. In this study, leaves and fruits of four olive cultivars including ‘Fishomi’, ‘Mary’, ‘Roghani’ and ‘Zard’ growing in the north of Iran were collected for comparative evaluation of the concentration of Oleuropein, Hydroxytyrosol and Tyrosol as well as antioxidant properties. According to the results, the highest and lowest levels of Tyrosol were detected in ‘Mary’ and ‘Zard’ leaves, respectively. There were significant differences between levels of Hydroxytyrosol in ‘Mary’ leaves compared with other examined cultivars. ‘Mary’ fruits had the highest level of Oleuropein, Hydroxytyrosol and Tyrosol, while ‘Fishomi’ fruits had the lowest level of these compounds. The total phenol content of leaf extracts was significantly higher than those detected in fruit extracts. In the leaves and fruits of the examined cultivars, the highest and the lowest Phenylalanine ammonia-lyase (PAL) activity was found in ‘Mary’ and ‘Fishomi’ cultivars, respectively. PAL activity in the leaves of all examined cultivars was significantly higher than their fruits. Meanwhile, the results showed that the antioxidant activities of the leaves in all cultivars were higher than the fruits. These results suggest that olive leaves, as well as fruits, might also be used as rich sources of natural antioxidants.
Ardavan Bakhshi Ganjeh, Mohammad Mehdi Rahimi, Abdolsamad Kelidari,
Volume 11, Issue 50 (vol. 11, no. 50 2022)
Abstract
In order to investigate the effects of salicylic acid and cycocel under drought stress conditions on agronomic and biochemical properties and essential oil of lemon balm, an experiment as a factorial split-plot in a randomized complete block design with three replications was carried out in northwest of Yasuj province during 2019 and 2020. Drought stress as the main factor included 35% (control), 70% and 90% field capacity, and the subplots included cycocel (0 and 1000 μM) and salicylic acid (0 and 1 μM). Results showed that foliar application of salicylic acid and cycocel had significant effect on grain yield, essential oil percentage, plant height, fresh and dry weight of shoot and fresh and dry weight of root and the amount of catalase, peroxidase and malondialdehyde enzymes. The highest yield belonged to irrigation treatment after application of 35% moisture along with foliar application with cyclocel and salicylic acid. Also, the most of essential oil yield belonged to irrigation treatment after consuming 70% moisture along with foliar application with cyclocel and salicylic acid. In general, the results showed that foliar application of salicylic acid and cyclocel caused drought resistance in lemon balm, and therefore it is recommended for areas similar to the climatic conditions of the study.
Maryam Akbari, Roya Razavizadeh, Hamid Sobhanian, Gholamreza Bakhshikhaniki, Gholamhossein Mohebbi,
Volume 11, Issue 51 (vol. 11, no. 51 2022)
Abstract
Some algae are rich sources of various beneficial lipids and fatty acids. In recent years, several studies have been performed on algae to evaluate the effect of different salinity treatments on the quantity and quality of these lipids. The present study was performed to investigate the effect of different concentrations of sodium chloride on total lipid content, fatty acid profile, and omega-3, 6, and 9 amounts in the alga Sargassum boveanum. Algae samples were collected from the shores of Bushehr Province and kept in three aquariums for 30 days in three control groups and treatments of 1 and 2 g / l sodium chloride. Then their lipid extraction was performed using Bligh and dyer method (1959). The factors of acid value (AV), peroxide value (PV), and refractive index (RI) of lipids were determined according to ISO standard methods for numbers 660 (2009), 3960 (2017), and 6320 (2017), respectively. The methylation of the samples was performed according to the standard method of 66-2-AOCS-Ce, and the analysis of fatty acids was performed by gas chromatography equipped with a flame ionization detector (GC-FID). The amount of fat in the treatment of one gram/liter of salt increased compared to the control group and then showed a decreasing trend in the treatment of two grams per liter; however, this difference was not significant. GC-FID analysis showed 17 types of fatty acids in each group. Among saturated and unsaturated fatty acids, palmitic acid and seronic acid showed the highest levels in all three groups, respectively. A slight increase in salinity caused a change in the values of omega-fatty acids. The findings of this study can be extended to achieve targeted mechanisms and conditions of salinity stress that increase specific fatty acids.
Elham Ghasemifar, Ghader Habibi, Golamreza Bakhshi-Khaniki,
Volume 11, Issue 52 (vol. 11, no. 52 2023)
Abstract
Since the use of seed priming agents to reduce the potential toxicity of AgNPs has not been explored, we evaluated the combined effects of 0.1 mM salicylic acid (SA) and 0.1 mM nitric oxide (NO) on responses of Hypericum perforatum plants to Ag-induced stress. The results of growth parameters together with measurement of malondialdehyde (MDA) revealed that exposure to AgNPs or AgNO3 caused more toxicity, which was closely associated with the over–accumulation of ROS. However, SNP (NO) and SA addition successfully mitigated adverse impact of AgNPs on H. perforatum seedlings, which was associated with the higher synthesis of chlorophyll b pigments and proline. In addition, a further increase in antioxidants (phenolic compounds) was observed in NO-primed plants under AgNPs-induced stress, which was concomitant with the high level of SOD activity. We concluded that NO minimized the inhibitory effects of AgNPs stress by increasing the phenolic compounds as well as by regulating accumulation of Ag, ROS and antioxidants. To sum up, exogenous NO plays a key role in enhancing its resistance to Ag stress in physiological base, thereby improving the growth and phenolic compounds production (chlorogenic acid, quercetin and rutin) of H. perforatum plant.
