In order to study of marmarine spraying effects on some physiological and antioxidant parameteres of tomato Lycopersicon esculentum cv. Teena, an experiment was conducted in complete randomized block design with 3 replications in field. Experiment treatments were three ascophyllum extract concentrations as (0, 1.5 and 3 ml/L) and four spraying stages of plant growth including (exactly after transplanting, exactly after primary flower production, exactly after primary fruit set and in all three phases of growth). Obtained results demonstrate that ascophyllum spraying has mean effect on total yield, chlorophyll a and b, relative water content, plant dry weight, fresh root weight. Marmarine treatment cause to increase of these parameters and most increasing was obtained in sparying of 3 ml/L ascophyllum concentration in all three phases of growth and after that in 3 ml/L at exactly after primary flowering stage. The most value of fruit ascorbic acid and phenolic content were obtained at treatment of marmarine extract 3 ml/L concentration when foliar spraying was set in all three phases of growth, simultaneously. Also, the most value of fruit anthocyanin content was obtained at marmarine 3 ml/L as foliar spraying was set exactly after primary fruit set.